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European Journal of Nutrition

, Volume 39, Issue 3, pp 121–125 | Cite as

Short-chain fatty acid (SCFA) uptake into Caco-2 cells by a pH-dependent and carrier mediated transport mechanism

  • J. Stein
  • M. Zores
  • O. Schröder
Original contribution

Summary

The short-chain fatty acids, acetate, propionate, and butyrate, are the most abundant organic anions in the human colon. SCFA play a pivotal role in maintaining homeostasis in the colon. Particularly butyrate induces cell differentiation and regulates growth and proliferation of colonic mucosal epithelial cells, whereas it reduces the growth rate of colorectal cancer cell. Previous studies by several groups, including our own, using isolated membrane vesicles have demonstrated that the uptake of butyrate is at least in part mediated by a non-electrogenic SCFA/HCO3 antiporter. The purpose of the present study was to determine (1) whether Caco-2 cells could serve as an experimental model to assess the mechanisms of SCFA transport, and (2) whether monocarboxylate transporters could play a role in SCFA transport in these cells. Caco-2 cells were found to transport 14C-butyrate in a concentration and time dependent manner. The uptake was sodium independent, but was stimulated by lowering extracellular pH. The uptake of 500μM butyrate was reduced by 49.6% ± 3.3% in the presence of propionate and by 57.2% ± 4.8% in the presence of 10 mM L-lactate. The addition of 1 mM α-cyano-4-hydroxycinnamate and phloretin, both known to be potent inhibitors of MCT1, decreased the uptake of 500 μM 14C-butyrate by 59.4% ± 4.1% and 48.9% ± 3.3%, respectively, whereas similar concentrations of DIDS did not have any effect. These data suggest that the uptake of butyrate in Caco-2 cells occurs via a carrier mediated transport system specific for monocarboxylic acids, which is in accordance with characteristics of the MCT1.

Key words Butyrate – Caco-2 cells – monocarboxylate transporter – carrier mediated transport 

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Copyright information

© Steinkopff Verlag 2000

Authors and Affiliations

  • J. Stein
    • 1
  • M. Zores
    • 1
  • O. Schröder
    • 1
  1. 1.Division of Gastroenterology, 2nd Department of Internal Medicine, Johann Wolfgang Goethe-University, Theodor-Stern-Kai 7, D-60590 Frankfurt, Germany, e-mail: J.Stein@em.uni-frankfurt.deDE

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