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Protective effects of Bifidobacterium bifidum FL-228.1 on dextran sulfate sodium-induced intestinal damage in mice

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Abstract

Purpose

Numerous studies have found that probiotics benefit the intestinal barrier. However, the prophylactic effects of probiotics on the intestinal barrier, i.e., if probiotics exert protective effects in healthy individuals to defend them against harmful elements, have seldomly been reported. The present study aimed to investigate the possible mechanisms of potential strains with the function of preventing intestinal barrier damage.

Methods

This study investigated nine potential probiotic strains using in vitro and in vivo models on their intestinal barrier-protecting properties. Transcriptomic was then employed to decipher the underlying mechanisms of action of the strains.

Results

The results showed that the strains, to varying degrees, regulated the ratio of interleukin (IL)-10 and IL-12 in peripheral blood mononuclear cells (PBMCs), increased the transepithelial electrical resistance (TEER) values, and decreased Caco-2 cell monolayers permeability. Correspondingly, the strains showed different prophylactic efficacies in protecting mice from dextran sulfate sodium (DSS)-induced intestinal barrier damage. Remarkably, Bifidobacterium bifidum FL-228.1 (FL-228.1) showed the best prophylactic efficacies in protecting mice from DSS-induced intestinal barrier damage. Further research suggested that FL-228.1 exerted its prophylactic effects by enhancing mucin 2 (Muc2) production and Claudin (Cldn)-4 in the colon. Furthermore, the transcriptomic and protein-protein interactions (PPI) analyses indicated that the inhibition of NLRP3 and the activation of PPARγ and TLR2 could be involved in protecting the intestinal barrier by FL-228.1.

Conclusion

Bifidobacterium bifidum FL-228.1 may be developed as a promising probiotic for the prevention of intestinal barrier damage via PPARγ/NLRP3/ TLR2 pathways by enhancing Muc2 and Cldn-4.

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Availability of data and materials

The data that support the findings of this study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Project of Taishan Industry Leading Talent of Shandong Province (LJNY202101), and the National Natural Science Foundation of China (Grant number: 31901664).

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Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China

    Rui Wang, Malina Kuerman, Qingyu Cui, Xiaoying Tian, Yu Zhou, Huaxi Yi, Pimin Gong, Kai Lin, Zhe Zhang, Tongjie Liu & Lanwei Zhang

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  1. Rui Wang
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Contributions

LZ and TL were in charge of conceptualization, funding acquisition and writing-review and editing; Investigating, methodology, visualization, and writing-original draft preparation were done by RW; MK, QC and XT were in charge of methodology; Data curation was done by R. W. and YZ; HY, PG, KL and ZZ were in charge of writing-review and editing. All authors have edited and approved the final manuscript.

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Correspondence to Tongjie Liu or Lanwei Zhang.

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The Laboratory Animal Ethics Committee of the College of Food Science and Engineering of Ocean University of China.

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Wang, R., Kuerman, M., Cui, Q. et al. Protective effects of Bifidobacterium bifidum FL-228.1 on dextran sulfate sodium-induced intestinal damage in mice. Eur J Nutr 62, 1267–1280 (2023). https://doi.org/10.1007/s00394-022-03064-x

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