Association of antenatal vitamin B complex supplementation with neonatal vitamin B12 status: evidence from a cluster randomized controlled trial



Evidence about the effect of maternal vitamin B12 supplementation on offspring’s vitamin B12 status is limited. The present interventional study sought to evaluate the association of antenatal vitamin B complex supplementation with neonatal vitamin B12 status.


In an ongoing cluster randomized controlled trial conducted in three rural counties in northwest China, pregnant women < 20 weeks of gestation were randomized to three treatment groups: blank control, iron supplements, or vitamin B complex supplements. All women were administered folic acid supplements during the periconceptional period. In a sub-study, we collected cord blood samples of 331 participants from the control or vitamin B complex groups in the Xunyi county from January 2017 to December 2017. Plasma concentrations of folate, vitamin B12, and homocysteine were measured. Linear mixed models with a random intercept for cluster were used to compare biochemical indexes between groups after controlling for covariates.


Compared with newborns whose mothers were in the control group, newborns of the vitamin B complex-supplemented women had significantly higher cord plasma vitamin B12 (P = 0.001) and lower homocysteine concentrations (P = 0.043). The association of antenatal vitamin B complex supplementation with cord blood vitamin B12 concentrations appeared to be more pronounced among newborns with high folate status than those with low folate status (Pinteraction = 0.060).


Maternal vitamin B complex supplementation during pregnancy was associated with better neonatal vitamin B12 status in rural northwest China.

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The authors thank all participants and researchers who participated in this study. The authors are also grateful to the Xunyi County Hospital and the Xunyi County Maternal and Child Health Hospital for their collaboration during the implementation of this trial.


This study was sponsored by the National Key Research and Development Program of China (grant number 2017YFC0907200, grant number 2017YFC0907201), the National Natural Science Foundation of China (grant number 81230016), the Project of birth defect control and prevention in Shaanxi (grant number Sxwsjswzfcght2016-013), the China Postdoctoral Science Foundation (grant number 2015M582678) and Xi’an Jiaotong University (xjj2018146). The funders had no role in the design of the study; in the collection, analyses, and interpretation of the data; in the writing of the manuscript, and in the decision to submit the manuscript for publication.

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Correspondence to Xin Liu or Shaonong Dang.

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Li, S., Mi, B., Qu, P. et al. Association of antenatal vitamin B complex supplementation with neonatal vitamin B12 status: evidence from a cluster randomized controlled trial. Eur J Nutr (2020).

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  • Antenatal vitamin B complex supplementation
  • Newborns
  • Vitamin B12 status
  • Cluster randomized controlled trial
  • Rural northwest china