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Protective properties of grape-seed proanthocyanidins in human ex vivo acute colonic dysfunction induced by dextran sodium sulfate

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Abstract

Purpose

Anti-inflammatory and barrier-protective properties have been attributed to proanthocyanidins in the context of intestinal dysfunction, however little information is available about the impact of these phytochemicals on intestinal barrier integrity and immune response in the human. Here we assessed the putative protective properties of a grape-seed proanthocyanidin extract (GSPE) against dextran sodium sulfate (DSS)-induced acute dysfunction of the human colon in an Ussing chamber system.

Methods

Human proximal and distal colon tissues from colectomized patients were submitted ex vivo for a 30-min preventive GSPE treatment (50 or 200 µg mL−1) followed by 1-h incubation with DSS (12% w v−1). Transepithelial electrical resistance (TEER), permeation of a fluorescently-labeled dextran (FD4) and proinflammatory cytokine release [tumor necrosis factor (TNF)-α and interleukin (IL)-1β] of colonic tissues were determined.

Results

DSS reduced TEER (45–52%) in both the proximal and distal colon; however, significant increments in FD4 permeation (fourfold) and TNF-α release (61%) were observed only in the proximal colon. The preventive GSPE treatment decreased DSS-induced TEER loss (20–32%), FD4 permeation (66–73%) and TNF-α release (22–33%) of the proximal colon dose-dependently. The distal colon was not responsive to the preventive treatment but showed a reduction in IL-1β release below basal levels with the highest GSPE concentration.

Conclusions

Our results demonstrate potential preventive effects of GSPE on human colon dysfunction. Further studies are required to test whether administering GSPE could be a complementary therapeutic approach in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.

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Acknowledgements

This research was funded by the Spanish Ministerio de Economía y Competitividad, Grants AGL2014-55347-R, AGL2017-83477-R, and 2014LINE-06 grant from the URV-Banco Santander. C. González-Quilen received financial support through a FI-AGAUR grant from the Generalitat de Catalunya, C. Grau-Bové is student fellow in the Martí i Franquès program of the Universitat Rovira i Virgili, Tarragona, Spain. M. Pinent and X. Terra are Serra-Húnter fellows at the Universitat Rovira i Virgili, Tarragona, Spain.

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  1. Ximena Terra and M. Teresa Blay contributed equally to this work.

Authors and Affiliations

  1. Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Universitat Rovira I Virgili, 43007, Tarragona, Spain

    Carlos González-Quilen, Carme Grau-Bové, Montserrat Pinent, Anna Ardévol, Raúl Beltrán-Debón, Ximena Terra & M. Teresa Blay

  2. Servei de Cirurgia General i de l’Aparell Digestiu, Hospital Universitari Joan XXIII, Tarragona, Spain

    Rosa Jorba-Martín & Aleidis Caro-Tarragó

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  1. Carlos González-Quilen
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  2. Carme Grau-Bové
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Correspondence to Raúl Beltrán-Debón.

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González-Quilen, C., Grau-Bové, C., Jorba-Martín, R. et al. Protective properties of grape-seed proanthocyanidins in human ex vivo acute colonic dysfunction induced by dextran sodium sulfate. Eur J Nutr 60, 79–88 (2021). https://doi.org/10.1007/s00394-020-02222-3

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