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Prebiotic supplementation modulates advanced glycation end-products (AGEs), soluble receptor for AGEs (sRAGE), and cardiometabolic risk factors through improving metabolic endotoxemia: a randomized-controlled clinical trial

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Abstract

Purpose

The oxidative stress plays a key role in the initiation, propagation, and development of the complications of type 2 diabetes mellitus (T2DM). This trial aimed to evaluate the effects of resistant dextrin as a prebiotic on the cardiometabolic risk factors and the status of oxidative stress in patients with T2DM.

Methods

Sixty-five female subjects with T2DM were assigned to either the intervention (n = 33) or control (n = 32) groups receiving 10 g/day of resistant dextrin or placebo, respectively, for 8 weeks. Fasting blood samples were collected at baseline and post-intervention to determine the serum levels of glycemic indices, lipid profile, atherogenic indices, and soluble receptor for AGEs (sRAGE), carboxymethyl lysine (CML), pentosidine, malondialdehyde (MDA), 8-iso-prostaglandin F2α (8-iso-PGF2α), total antioxidant capacity (TAC), antioxidant enzymes activity, and uric acid. Data were analyzed using SPSS software 17. Paired, unpaired Student’s t tests, and analysis of covariance were used to compare the quantitative variables.

Results

Resistant dextrin caused a significant decrease in FPG (− 17.43 mg/dl, 9.80%), TG (− 40.25 mg/dl, 23.01%), TC/HDL (− 0.80, 21.87%), LDL-c/HDL-c (− 0.80, 17.85%), Atherogenic index (− 0.40, 15.80%), LPS (− 6.5 EU/ml, 23.40%) and hs-CRP (− 8.02 ng/ml, 54.00%), MDA (− 1.21 nmol/mL, 25.58%), CML (− 93.40 ng/ml, 26.30%), 8-iso-PGF2α (− 4.65 pg/ml, 15.00%), and a significant increase in TAC (0.33 mmol/L, 36.25%) and s-RAGE (2.10 ng/ml, 28.90%) in the intervention group compared with the control group. No significant changes were observed in glycosylated hemoglobin, total cholesterol, LDL-c, HDL-c, superoxide dismutase, glutathione peroxidase and catalase, pentosidine, and uric acid in the intervention group compared with the control group.

Conclusions

Supplementation with resistant dextrin may improve the advanced glycation end-products, sRAGE, and cardiometabolic risk factors in women with type 2 diabetes mellitus.

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Abbreviations

AGEs:

Advanced glycation end-products

ANCOVA:

Analysis of covariance

CML:

Carboxymethyl lysine

ELISA:

Enzyme-linked immunosorbent assay

GLP-1:

Glucagon-like peptide1

GSH-Px:

Glutathione peroxidase

GSSG:

Reduced oxidized glutathione

HbA1c:

Glycosylated hemoglobin

HMG-CoA:

3-hydroxy-3-methylglutaryl-CoA

HOMA-IR:

Homeostatic Model Assessment for Insulin Resistance

hs-CRP:

High-sensitivity C-reactive protein

IDF:

International diabetes federation

LPS:

Lipopolysaccharide

MAPK:

Mitogen-activated protein kinase

MDA:

Malondialdehde

NF-κB:

Factor κB

PYY:

Peptide YY

RAS:

Random allocation software

ROS:

Reactive oxygen species

SCFA:

Short-chain fatty acids

SOD:

Superoxide dismutase

sRAGE:

Soluble receptor of advanced glycation end-products

T2DM:

Type 2 diabetes mellitus

TAC:

Total antioxidant capacity

TBA:

Thiobarbituric acid

TLR 4:

Toll-like receptor 4

TNF-α:

Tumor necrosis factor-alpha

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Acknowledgements

The authors would like to thank all the patients and Mr. Firuz Purrahim for their cooperation. The authors would like to thank all of the participants. The Nutrition faculty, the Nutrition Research Center, and the Vice Chancellor of Research of Tabriz University of Medical Sciences, Iran, financially supported this research.

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Authors and Affiliations

  1. Drug Applied Research Center, Nutrition Research Center, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahdieh Abbasalizad Farhangi

  2. Nutrition Research Center, Immunology Research Center, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Parvin Dehghan

  3. Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Nazli Namazi

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  1. Mahdieh Abbasalizad Farhangi
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Research project: PD, NN, and MAF. Statistical analysis: MAF and PD. Manuscript preparation: PD, NN. Review and critique: PD, MAF, and NN.

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Correspondence to Parvin Dehghan.

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Farhangi, M.A., Dehghan, P. & Namazi, N. Prebiotic supplementation modulates advanced glycation end-products (AGEs), soluble receptor for AGEs (sRAGE), and cardiometabolic risk factors through improving metabolic endotoxemia: a randomized-controlled clinical trial. Eur J Nutr 59, 3009–3021 (2020). https://doi.org/10.1007/s00394-019-02140-z

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