Beneficial effects of ginger on prevention of obesity through modulation of gut microbiota in mice



Recent evidence has demonstrated that the gut microbiota plays a critical role in the treatment of obesity and other metabolic dysfunctions. Ginger (Zingiber officinale Roscoe), one of the most commonly used spices and dietary supplements, has been shown to exert beneficial effects against obesity and related disorders. However, to date, the mechanisms linking these effects to the gut microbiota remain unclear. This study aims to investigate the relationship between the gut microbiota and the metabolic adaptations resulting from ginger supplementation in mice.


Four groups of mice were fed a normal chow diet (NCD) or a high-fat diet (HFD) with or without ginger supplementation for 16 weeks. Lipid profiles, proinflammatory cytokines, glucose tolerance, microbiota composition and short-chain fatty acid (SCFA) concentrations were analyzed at the end of the experiment. In addition, microbiota-depleted mice were transplanted with the fecal microbiota of mice fed a HFD or mice fed a HFD along with ginger supplementation. Glucose tolerance and microbiota composition were assessed after a 8-week fecal microbiota transplantation (FMT).


We observed marked decreases in body weight, liver steatosis, and low-grade inflammation as well as amelioration of insulin resistance in the HFD-fed mice treated with ginger. Furthermore, ginger supplementation modulated the gut microbiota composition and increased species belonging to the Bifidobacterium genus and SCFA-producing bacteria (Alloprevotella and Allobaculum), along with increases in fecal SCFA concentrations. The FMT experiment showed anti-obesity and microbiota-modulating effects similar to those observed in the oral ginger-feeding experiment.


This study suggests that modulation of the gut microbiota as a result of ginger supplementation has a therapeutic effect on obesity in mice.

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  1. 1.

    World Health Organization (2018) Obesity and overweight. Accessed 13 Mar 2018

  2. 2.

    Brahe LK, Astrup A, Larsen LH (2016) Can we prevent obesity-related metabolic diseases by dietary modulation of the gut microbiota? Adv Nutr 7(1):90–101.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Schroeder BO, Bäckhed F (2016) Signals from the gut microbiota to distant organs in physiology and disease. Nat Med 22(10):1079–1089.

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Zhao L (2013) The gut microbiota and obesity: from correlation to causality. Nat Rev Microbiol 11(9):639–647.

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Suárezzamorano N, Fabbiano S, Chevalier C et al (2015) Microbiota depletion promotes browning of white adipose tissue and reduces obesity. Nat Med 21(12):1497–1501.

    CAS  Article  Google Scholar 

  6. 6.

    Qin J, Li R, Raes J et al (2010) A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464(7285):59–65.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Rosenbaum M, Knight R, Leibel RL (2015) The gut microbiota in human energy homeostasis and obesity. Trends Endocrinol Metab 26(9):493.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. 8.

    Cani PD, Amar J, Iglesias MA et al (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56(7):1761–1772.

    CAS  Article  Google Scholar 

  9. 9.

    Bindels LB, Delzenne NM, Cani PD et al (2015) Towards a more comprehensive concept for prebiotics. Nat Rev Gastroenterol Hepatol 12(5):303–310.

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Tremaroli V, Bäckhed F (2012) Functional interactions between the gut microbiota and host metabolism. Nature 489(7415):242–249.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Bartley JP, Jacobs AL (2000) Effects of drying on flavour compounds in Australian-grown ginger (Zingiber officinale). J Sci Food Agric 80(2):209–215.;2-8

    CAS  Article  Google Scholar 

  12. 12.

    Li Y, Hong Y, Han Y et al (2016) Chemical characterization and antioxidant activities comparison in fresh, dried, stir-frying and carbonized ginger. J Chromatogr B Anal Technol Biomed Life Sci 1011:223–232.

    CAS  Article  Google Scholar 

  13. 13.

    Ezzat SM, Ezzat MI, Okba MM et al (2018) The hidden mechanism beyond ginger (Zingiber officinale Rosc.) potent in vivo and in vitro anti-inflammatory activity. J Ethnopharmacol 214:113–123.

    Article  PubMed  Google Scholar 

  14. 14.

    Lee SH, Cekanova M, Baek SJ (2008) Multiple mechanisms are involved in 6-gingerol-induced cell growth arrest and apoptosis in human colorectal cancer cells. Mol Carcinog 47(3):197–208.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Lee JO, Kim N, Lee HJ et al (2015) 6-Gingerol affects glucose metabolism by dual regulation via the AMPKα2-mediated AS160–Rab5 pathway and AMPK-mediated insulin sensitizing effects. J Cell Biochem 116(7):1401–1410.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Tohma H, Gülçin İ, Bursal E et al (2016) Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS/MS. J Food Meas Charact 11(2):556–566.

    Article  Google Scholar 

  17. 17.

    Misawa K, Hashizume K, Yamamoto M et al (2015) Ginger extract prevents high-fat diet-induced obesity in mice via activation of the peroxisome proliferator-activated receptor δ pathway. J Nutr Biochem 26(10):1058–1067.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Sani NFA, Belani LK, Chong PS et al (2014) Effect of the combination of gelam honey and ginger on oxidative stress and metabolic profile in streptozotocin-induced diabetic sprague-dawley rats. Biomed Res Int 2014(4):160695.

    Article  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Akinyemi AJ, Thome GR, Morsch VM et al (2015) Effect of dietary supplementation of ginger and turmeric rhizomes on ectonucleotidases, adenosine deaminase and acetylcholinesterase activities in synaptosomes from the cerebral cortex of hypertensive rats. J Appl Biomed 14(1):59–70.

    Article  Google Scholar 

  20. 20.

    Gao H, Guan T, Li C et al (2012) Treatment with ginger ameliorates fructose-induced fatty liver and hypertriglyceridemia in rats: modulation of the hepatic carbohydrate response element-binding protein-mediated pathway. Evid Based Complement Altern Med 2012:570948.

    Article  Google Scholar 

  21. 21.

    Wang J, Ke W, Bao R et al (2017) Beneficial effects of ginger Zingiber officinale Roscoe on obesity and metabolic syndrome: a review. Ann N Y Acad Sci 1398(1):83–98.

    Article  PubMed  Google Scholar 

  22. 22.

    Amato KR, Yeoman CJ, Kent A et al (2013) Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes. ISME J 7(7):1344–1353.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Segata N, Izard J, Waldron L et al (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12(6):R60.

    Article  PubMed  PubMed Central  Google Scholar 

  24. 24.

    Langille MG, Zaneveld J, Caporaso JG et al (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31(9):814–821.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. 25.

    Yu H, Guo Z, Shen S et al (2016) Effects of taurine on gut microbiota and metabolism in mice. Amino Acids 48(7):1601–1617.

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Chang CJ, Lin CS, Lu CC, et al (2015) Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun 6(163):1360–1374.

    CAS  Article  Google Scholar 

  27. 27.

    Li G, Xie C, Lu S, et al (2017) Intermittent fasting promotes white adipose browning and decreases obesity by shaping the gut microbiota. Cell Metab 4(26):672–685.

    CAS  Article  Google Scholar 

  28. 28.

    Matsuda A, Wang ZZ, Takahashi S et al (2009) Upregulation of mRNA of retinoid binding protein and fatty acid binding protein by cholesterol enriched-diet and effect of ginger on lipid metabolism. Life Sci 84:903–907.

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Wellen KE, Fucho R, Gregor MF et al (2007) Coordinated regulation of nutrient and inflammatory responses by STAMP2 is essential for metabolic homeostasis. Cell 129(3):451–452.

    CAS  Article  Google Scholar 

  30. 30.

    Fontana L, Eagon JC, Trujillo ME et al (2007) Visceral fat adipokine secretion is associated with systemic inflammation in obese humans. Diabetes 56(4):1010–1013.

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Boulangé CL, Neves AL, Chilloux J et al (2016) Impact of the gut microbiota on inflammation, obesity, and metabolic disease. Genome Med 8(1):42.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Tzeng TF, Liou SS, Chang CJ et al (2015) 6-Gingerol dampens hepatic steatosis and inflammation in experimental nonalcoholic steatohepatitis. Phytomedicine 22(4):452–461.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Nammi S, Sreemantula S, Roufogalis BD (2010) Protective effects of ethanolic extract of Zingiber officinale rhizome on the development of metabolic syndrome in high-fat diet-fed rats. Basic Clin Pharmacol Toxicol 104(5):366–373.

    CAS  Article  Google Scholar 

  34. 34.

    Brahmanaidu P, Uddandrao VV, Ravindarnaik RC et al (2015) Ameliorative potential of gingerol: Promising modulation of inflammatory factors and lipid marker enzymes expressions in HFD induced obesity in rats. Mol Cell Endocrinol 419(5):139–147.

    CAS  Article  Google Scholar 

  35. 35.

    Yu Y, Zick S, Li X et al (2011) Examination of the pharmacokinetics of active ingredients of ginger in humans. The AAPS J 13(3):417.

    CAS  Article  PubMed  Google Scholar 

  36. 36.

    Arora T, Bäckhed F (2016) The gut microbiota and metabolic disease: current understanding and future perspectives. J Intern Med 280(4):339–349.

    CAS  Article  PubMed  Google Scholar 

  37. 37.

    Zhang X, Zhao Y, Xu J et al (2015) Modulation of gut microbiota by berberine and metformin during the treatment of high-fat diet-induced obesity in rats. Sci Rep 5(6):14405.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  38. 38.

    Barczynska R, Jurgoński A, Slizewska K et al (2017) Effects of potato dextrin on the composition and metabolism of the gut microbiota in rats fed standard and high-fat diets. J Funct Foods 34:398–407.

    CAS  Article  Google Scholar 

  39. 39.

    Ming L, Wang Y, Fan G et al (2017) Balancing herbal medicine and functional food for prevention and treatment of cardiometabolic diseases through modulating gut microbiota. Front Microbiol 8:2146.

    Article  Google Scholar 

  40. 40.

    Schultz M, Timmer A, Herfarth HH et al (2004) Lactobacillus GG in inducing and maintaining remission of Crohn’s disease. BMC Gastroenterol 4(1):5.

    Article  PubMed  PubMed Central  Google Scholar 

  41. 41.

    Zhang S, Yang J, Henning SM et al (2017) Dietary pomegranate extract and inulin affect gut microbiome differentially in mice fed an obesogenic diet. Anaerobe 48:184–193.

    CAS  Article  PubMed  Google Scholar 

  42. 42.

    Cani PD, Neyrinck AM, Fava F et al (2007) Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia. Diabetologia 50(11):2374–2383.

    CAS  Article  PubMed  Google Scholar 

  43. 43.

    Zhang CH, Zhang MH, Wang SY et al (2010) Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice. ISME J 4(2):232–241.

    CAS  Article  PubMed  Google Scholar 

  44. 44.

    Everard A, Lazarevic V, Gaïa N et al (2014) Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity. ISME J 8(10):2116–2130.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Kim KA, Gu W, Lee IA et al (2012) High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway. PLoS One 7(10):e47713.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  46. 46.

    Sen T, Cawthon CR, Ihde BT et al (2017) Diet-driven microbiota dysbiosis is associated with vagal remodeling and obesity. Physiol Behav 173:305–317.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  47. 47.

    Duca FA, Sakar Y, Lepage P et al (2014) Replication of obesity and associated signaling pathways through transfer of microbiota from obese-prone rats. Diabetes 63(5):1624–1636.

    CAS  Article  PubMed  Google Scholar 

  48. 48.

    Xiao S, Fei N, Pang X et al (2014) A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. FEMS Microbiol Ecol 87(2):357–367.

    CAS  Article  PubMed  Google Scholar 

  49. 49.

    Canfora EE, Jocken JW, Blaak EE (2015) Short-chain fatty acids in control of body weight and insulin sensitivity. Nat Rev Endocrinol 11(10):577.

    CAS  Article  PubMed  Google Scholar 

  50. 50.

    Chang DH, Rhee MS, Ahn S et al (2015) Erratum to: Faecalibaculum rodentium gen. nov., sp. nov., isolated from the faeces of a laboratory mouse. Antonie Van Leeuwenhoek 108(6):1–1.

    CAS  Article  Google Scholar 

  51. 51.

    Tsai YT, Cheng PC, Pan TM (2014) Anti-obesity effects of gut microbiota are associated with lactic acid bacteria. Appl Microbiol Biotechnol 98(1):1–10.

    CAS  Article  PubMed  Google Scholar 

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This work was supported by the Beijing Municipal Science and Technology Project (Grant number D16110500540001).

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Correspondence to Fang Chen.

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Wang, J., Wang, P., Li, D. et al. Beneficial effects of ginger on prevention of obesity through modulation of gut microbiota in mice. Eur J Nutr 59, 699–718 (2020).

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  • Ginger
  • Gut microbiota
  • Obesity
  • Short-chain fatty acid
  • Fecal microbiota transplantation