Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study

Abstract

Purpose

Older individuals suffer from low NADH levels. We have previously shown that nicotinamide riboside [NR; a NAD(P)(H) precursor] administration impaired exercise performance in young rats. It has been suggested that supplementation of redox agents exerts ergogenic effect only in deficient individuals. We hypothesized that old individuals would more likely benefit from NR supplementation. We investigated the effect of acute NR supplementation on redox homeostasis and physical performance in young and old individuals.

Methods

Twelve young and twelve old men received NR or placebo in a double-blind cross-over design. Before and 2 h after NR or placebo supplementation, blood and urine samples were collected, while physical performance (VO2max, muscle strength, and fatigue) was assessed after the second blood sample collection.

Results

At rest, old individuals exhibited lower erythrocyte NAD(P)H levels, higher urine F2-isoprostanes and lower erythrocyte glutathione levels compared to young (P < 0.05). NR supplementation increased NADH (51% young; 59% old) and NADPH (32% young; 38% old) levels in both groups (P < 0.05), decreased F2-isoprostanes by 18% (P < 0.05), and tended to increase glutathione (P = 0.078) only in the old. NR supplementation did not affect VO2max and concentric peak torque, but improved isometric peak torque by 8% (P = 0.048) and the fatigue index by 15% (P = 0.012) in the old. In contrast, NR supplementation did not exert any redox or physiological effect in the young.

Conclusions

NR supplementation increased NAD(P)H levels, decreased oxidative stress, and improved physical performance only in old subjects, substantiating that redox supplementation may be beneficial only in individuals with antioxidant deficiencies.

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References

  1. 1.

    Bieganowski P, Brenner C (2004) Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss–Handler independent route to NAD+ in fungi and humans. Cell 117:495–502. https://doi.org/10.1016/S0092-8674(04)00416-7

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Agledal L, Niere M, Ziegler M (2010) The phosphate makes a difference: cellular functions of NADP. Redox Rep 15:2–10. https://doi.org/10.1179/174329210X12650506623122

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Cantó C, Houtkooper RH, Pirinen E, Youn DY, Oosterveer MH, Cen Y, Fernandez-Marcos PJ, Yamamoto H, Andreux PA, Cettour-Rose P, Gademann K (2012) The NAD+ precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. Cell Metab 15:838–847. https://doi.org/10.1016/j.cmet.2012.04.022

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Trammell SA, Weidemann BJ, Chadda A, Yorek MS, Holmes A, Coppey LJ, Obrosov A, Kardon RH, Yorek MA, Brenner C (2016) Nicotinamide riboside opposes type 2 diabetes and neuropathy in mice. Sci Rep 6:26933. https://doi.org/10.1038/srep26933

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Vaur P, Brugg B, Mericskay M, Li Z, Schmidt MS, Vivien D, Orset C, Jacotot E, Brenner C, Duplus E (2017) Nicotinamide riboside, a form of vitamin B3, protects against excitotoxicity-induced axonal degeneration. FASEB J 31:5440–5452. https://doi.org/10.1096/fj.201700221RR

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Frederick DW, Loro E, Liu L, Davila A, Chellappa K, Silverman IM, Quinn WJ 3rd, Gosai SJ, Tichy ED, Davis JG, Mourkioti F, Gregory BD, Dellinger RW, Redpath P, Migaud ME, Nakamaru-Ogiso E, Rabinowitz JD, Khurana TS, Baur JA (2016) Loss of NAD homeostasis leads to progressive and reversible degeneration of skeletal muscle. Cell Metab 24:269–282. https://doi.org/10.1016/j.cmet.2016.07.005

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Zhang H, Ryu D, Wu Y, Gariani K, Wang X, Luan P, D’Amico D, Ropelle ER, Lutolf MP, Aebersold R, Schoonjans K, Menzies KJ, Auwerx J (2016) NAD+ repletion improves mitochondrial and stem cell function and enhances life span in mice. Science 352:1436–1443. https://doi.org/10.1126/science.aaf2693

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Kourtzidis IA, Stoupas AT, Gioris IS, Veskoukis AS, Margaritelis NV, Tsantarliotou M, Taitzoglou I, Vrabas IS, Paschalis V, Kyparos A, Nikolaidis MG (2016) The NAD+ precursor nicotinamide riboside decreases exercise performance in rats. J Int Soc Sports Nutr 13:32. https://doi.org/10.1186/s12970-016-0143-x

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Kourtzidis IA, Dolopikou CF, Tsiftsis AN, Margaritelis NV, Theodorou AA, Zervos IA, Tsantarliotou MP, Veskoukis AS, Vrabas IS, Paschalis V, Kyparos A, Nikolaidis MG (2018) Nicotinamide riboside supplementation dysregulates redox and energy metabolism in rats: implications for exercise performance. Exp Physiol. https://doi.org/10.1113/EP086964

    Article  PubMed  Google Scholar 

  10. 10.

    Mouchiroud L, Houtkooper RH, Auwerx J (2013) NAD+ metabolism: a therapeutic target for age-related metabolic disease. Crit Rev Biochem Mol Biol 48:397–408. https://doi.org/10.3109/10409238.2013.789479

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Yoshino J, Mills KF, Yoon MJ, Imai SI (2011) Nicotinamide mononucleotide, a key NAD+ intermediate, treats the pathophysiology of diet-and age-induced diabetes in mice. Cell Metab 14:528–536. https://doi.org/10.1016/j.cmet.2011.08.014

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Margaritelis NV, Paschalis V, Theodorou AA, Kyparos A, Nikolaidis MG (2018) Antioxidants in personalized nutrition and exercise. Adv Nutr. https://doi.org/10.1093/advances/nmy052

    Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Paschalis V, Theodorou AA, Kyparos A, Dipla K, Zafeiridis A, Panayiotou G, Vrabas IS, Nikolaidis MG (2016) Low vitamin C values are linked with decreased physical performance and increased oxidative stress: reversal by vitamin C supplementation. Eur J Nutr 55:45–53. https://doi.org/10.1007/s00394-014-0821-x

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Paschalis V, Theodorou AA, Margaritelis NV, Kyparos A, Nikolaidis MG (2018) N-Acetylcysteine supplementation increases exercise performance and reduces oxidative stress only in individuals with low levels of glutathione. Free Radic Biol Med 115:288–297. https://doi.org/10.1016/j.freeradbiomed.2017.12.007

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Bonkowski MS, Sinclair DA (2016) Slowing ageing by design: the rise of NAD+ and sirtuin-activating compounds. Nat Rev Mol Cell Biol 17:679. https://doi.org/10.1038/nrm.2016.93

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Reid MB (2016) Redox interventions to increase exercise performance. J Physiol 594:5125–5133. https://doi.org/10.1113/JP270653

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Schultz MB, Sinclair DA (2016) Why NAD+ declines during aging: it’s destroyed. Cell Metab 23:965–966. https://doi.org/10.1016/j.cmet.2016.05.022

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Verdin E (2015) NAD+ in aging, metabolism, and neurodegeneration. Science 350:1208–1213. https://doi.org/10.1126/science.aac4854

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Trammell SA, Schmidt MS, Weidemann BJ, Redpath P, Jaksch F, Dellinger RW, Li Z, Abel ED, Migaud ME, Brenner C (2016) Nicotinamide riboside is uniquely and orally bioavailable in mice and humans. Nat Commun 7:12948. https://doi.org/10.1038/ncomms12948

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Golding LA, Myers CR, Sinning WE (eds) (1982) Y’s way to physical fitness. YMCA of the USA, Chicago

    Google Scholar 

  21. 21.

    Beekley MD, Brechue WF, Dehoyos DV, Garzarella L, Werber-Zion G, Pollock ML (2004) Cross-validation of the YMCA submaximal cycle ergometer test to predict VO2max. Res Q Exerc Sport 75:337–342. https://doi.org/10.1080/02701367.2004.10609165

    Article  PubMed  Google Scholar 

  22. 22.

    Jamnick NA, Pettitt CD, Pettitt RW (2016) Comparison of the YMCA and a Custom submaximal exercise test for determining VO2max. Med Sci Sports Exerc 48:254–259. https://doi.org/10.1249/MSS.0000000000000763

    Article  PubMed  Google Scholar 

  23. 23.

    Veskoukis AS, Kyparos A, Paschalis V, Nikolaidis MG (2016) Spectrophotometric assays for measuring redox biomarkers in blood. Biomarkers 21:208–217. https://doi.org/10.3109/1354750X.2015.1126648

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    Veskoukis AS, Margaritelis NV, Kyparos A, Paschalis V, Nikolaidis MG (2018) Spectrophotometric assays for measuring redox biomarkers in blood and tissues: the NADPH network. Redox Rep 23:47–56. https://doi.org/10.1080/13510002.2017.1392695

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Wellek S, Blettner M (2012) On the proper use of the crossover design in clinical trials: part 18 of a series on evaluation of scientific publications. Dtsch Arztebl Int 109:276–281. https://doi.org/10.3238/arztebl.2012.0276

    Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Airhart SE, Shireman LM, Risler LJ, Anderson GD, Gowda GN, Raftery D, Tian R, Shen DD, O’Brien KD (2017) An open-label, non-randomized study of the pharmacokinetics of the nutritional supplement nicotinamide riboside (NR) and its effects on blood NAD+ levels in healthy volunteers. PloS one 12:e0186459. https://doi.org/10.1371/journal.pone.0186459

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  27. 27.

    Dellinger RW, Santos SR, Morris M, Evans M, Alminana D, Guarente L, Marcotulli E (2017) Repeat dose NRPT (nicotinamide riboside and pterostilbene) increases NAD+ levels in humans safely and sustainably: a randomized, double-blind, placebo-controlled study. NPJ Aging Mech Dis 3:17. https://doi.org/10.1038/s41514-017-0016-9

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  28. 28.

    Martens CR, Denman BA, Mazzo MR, Armstrong ML, Reisdorph N, McQueen MB, Chonchol M, Seals DR (2018) Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD+ in healthy middle-aged and older adults. Nat Commun 9:1286. https://doi.org/10.1038/s41467-018-03421-7

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  29. 29.

    Nikolaidis MG, Kyparos A, Vrabas IS (2011) F2-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res 50:89–103. https://doi.org/10.1016/j.plipres.2010.10.002

    CAS  Article  PubMed  Google Scholar 

  30. 30.

    van’t Erve TJ, Kadiiska MB, London SJ, Mason RP (2017) Classifying oxidative stress by F2-isoprostane levels across human diseases: a meta-analysis. Redox Biol 12:582–599. https://doi.org/10.1016/j.redox.2017.03.024

    CAS  Article  Google Scholar 

  31. 31.

    Margaritelis NV, Cobley JN, Paschalis V, Veskoukis AS, Theodorou AA, Kyparos A, Nikolaidis MG (2016) Principles for integrating reactive species into in vivo biological processes: examples from exercise physiology. Cell Signal 28:256–271. https://doi.org/10.1016/j.cellsig.2015.12.011

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Reid MB (2001) Invited Review: redox modulation of skeletal muscle contraction: what we know and what we don’t. J Appl Physiol 90:724–731. https://doi.org/10.1152/jappl.2001.90.2.724

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Margaritelis NV, Veskoukis AS, Paschalis V, Vrabas IS, Dipla K, Zafeiridis A, Kyparos A, Nikolaidis MG (2015) Blood reflects tissue oxidative stress: a systematic review. Biomarkers 20:97–108. https://doi.org/10.3109/1354750X.2014.1002807

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Pamp K, Bramey T, Kirsch M, De Groot H, Petrat F (2005) NAD(H) enhances the Cu(II)-mediated inactivation of lactate dehydrogenase by increasing the accessibility of sulfhydryl groups. Free Radic Res 39:31–40. https://doi.org/10.1080/10715760400023671

    CAS  Article  PubMed  Google Scholar 

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Correspondence to Michalis G. Nikolaidis.

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Dolopikou, C.F., Kourtzidis, I.A., Margaritelis, N.V. et al. Acute nicotinamide riboside supplementation improves redox homeostasis and exercise performance in old individuals: a double-blind cross-over study. Eur J Nutr 59, 505–515 (2020). https://doi.org/10.1007/s00394-019-01919-4

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Keywords

  • Ergogenic supplements
  • Exercise physiology
  • Performance
  • Sports nutrition