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Bifidobacterium pseudocatenulatum CECT 7765 supplementation improves inflammatory status in insulin-resistant obese children



The relationships between gut microbiota and obesity-related co-morbidities have been increasingly recognized. Low-grade inflammation may be the main factor in the pathogenesis of such disorders. We investigated the effect of the potential probiotic Bifidobacterium pseudocatenulatum CECT 7765 on cardiometabolic risk factors, inflammatory cytokines and gut microbiota composition in obese children with insulin resistance.


The study included 48 obese children (10–15 years old) with insulin resistance. They received dietary advice and were assigned to take the capsules with or without probiotic (109−10 CFU) daily for 13 weeks. Clinical, biochemical and gut microbiome measurements were made at baseline and at the end of the intervention.


There was a significant improvement in body mass index in all children after the intervention, suggesting that weight changes are related to the dietary advice. A significant decrease in circulating high-sensitive C-reactive protein (P = 0.026) and monocyte chemoattractant protein-1 (P = 0.032) and an increase in high-density lipoprotein cholesterol (P = 0.035) and omentin-1 (P = 0.023) in children receiving probiotic supplementation were observed compared to the control group. Regarding gut microbiota, probiotic administration significantly increased the proportion of the Rikenellaceae family members, particularly of the Alistipes genus.


The beneficial effects of the intervention on inflammatory markers and lipid profile suggest that B. pseudocatenulatum CECT 7765 intake together with dietary recommendations can improve inflammatory status in children with obesity and insulin resistance. These effects are parallel to increases in bacterial groups associated with a lean phenotype. The modulation of gut microbiota with probiotic supplementation can be considered an effective tool to ameliorate some obesity-related disorders in children.

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This study was supported by Grant AGL2014-52101-P from the Spanish Ministry of Economy and Competitiveness. The contract of Jesús Sanchis-Jordá was supported by a grant from the Spanish Training University Lecturers Programme (Formación de Profesorado Universitario) from the Spanish Ministry of Education, Culture and Sports (Grant number FPU13/03753). The contracts of Eva M. Gómez del Pulgar and Alfonso Benítez-Páez were supported by the EU Project MyNewGut (no. 613979) from the 7th Framework Program.

Author information




JS-C, PC-F, and JC-L performed the children enrollment, dietary counseling during the study, and statistical analysis of clinical data. EMGP and AB-P accomplished the analysis of gut microbiota. YS and PC-F designed and directed the study.

Corresponding author

Correspondence to Pilar Codoñer-Franch.

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The authors declare that they have no conflict of interest.

Ethical approval

The protocol of the study was approved by the Dr. Peset University Hospital ethics committee. All procedures in the study were in complete accordance with the Helsinki declaration and its later amendments.

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Sanchis-Chordà, J., del Pulgar, E.M.G., Carrasco-Luna, J. et al. Bifidobacterium pseudocatenulatum CECT 7765 supplementation improves inflammatory status in insulin-resistant obese children. Eur J Nutr 58, 2789–2800 (2019).

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  • Bifidobacterium pseudocatenulatum
  • Children obesity
  • Gut microbiota
  • Monocyte chemoattractant protein-1
  • Omentin-1
  • Probiotics