The impact of probiotic supplementation during pregnancy on DNA methylation of obesity-related genes in mothers and their children
- 879 Downloads
Dietary supplementation with probiotics during pregnancy has been suggested to decrease the risk for obesity in women after delivery and to minimize excessive weight gain in their children. Epigenetic DNA methylation has been proposed to impact on gene activity, thereby providing a plausible molecular mechanism for a broad range of biological processes and diseases. This pilot study aimed to evaluate whether probiotic supplementation during pregnancy could modify the DNA methylation status of the promoters of obesity and weight gain-related genes in mothers and their children.
A sample of 15 pregnant women was taken from a prospective, randomized mother and infant nutrition and probiotic study. Seven women received the probiotic supplementation and eight served as controls. The women’s and their children’s DNA methylation status of obesity (623 genes) and weight gain-related (433) gene promoters were analyzed from blood samples at the mean of 9.8 months (range 6.1–12.7 months) postpartum.
Probiotic supplementation led to significantly decreased levels of DNA methylation in 37 gene promoters and increased levels of DNA methylation in one gene promoter in women. In their children, 68 gene promoters were significantly affected consistently with a lower level of DNA methylation in the probiotic group.
On the basis of our pilot study, we suggest that probiotic supplementation during pregnancy may affect the DNA methylation status of certain promoters of obesity and weight gain-related genes both in mothers and their children, thereby providing a potential mechanism for long-lasting health effects.
KeywordsProbiotic Pregnancy Diet Obesity Methylation
The present study was supported by the grants from the Social Insurance Institution of Finland, the Päivikki and Sakari Sohlberg Foundation, the Jenny and Antti Wihuri Foundation (personal grant to S. V.), the Juho Vainio Foundation (personal grant to S. V.), and Finnish Cultural Foundation the Varsinais-Suomi Regional Fund (personal grant to S.V.) The Turku Centre for Biotechnology was funded by Biocenter Finland, University of Turku and Åbo Akademi. The food products were provided by Raisio plc (Raisio), but the company had no influence on the design or reporting of the study. Furthermore, we would like to thank our two research nurse, Ulla-Maija Eriksson for the clinical work she conducted with the study subjects and Satu Leinonen for her technical assistance.
Compliance with ethical standards
Conflict of interest
None of the authors have any conflict of interest to declare.
The authors’ responsibilities were as follows: SV, KL, EI, and SS designed the research and SV, KL, EI, SS, RL, and AL conducted the research. All of the authors participated in the preparation of the manuscript and are responsible for the final content.
- 3.Wu GD, Chen J, Hoffmann C, Bittinger K, Chen YY, Keilbaugh SA, Bewtra M, Knights D, Walters WA, Knight R, Sinha R, Gilroy E, Gupta K, Baldassano R, Nessel L, Li H, Bushman FD, Lewis JD (2011) Linking long-term dietary patterns with gut microbial enterotypes. Science 334:105–108CrossRefGoogle Scholar
- 12.Piirainen T, Isolauri E, Lagstrom H, Laitinen K (2006) Impact of dietary counselling on nutrient intake during pregnancy: a prospective cohort study. Br J Nutr 96:1095–1104Google Scholar
- 20.Singh A, Hacini-Rachinel F, Gosoniu ML, Bourdeau T, Holvoet S, Doucet-Ladeveze R, Beaumont M, Mercenier A, Nutten S (2013) Immune-modulatory effect of probiotic Bifidobacterium lactis NCC2818 in individuals suffering from seasonal allergic rhinitis to grass pollen: an exploratory, randomized, placebo-controlled clinical trial. Eur J Clin Nutr 67:161–167CrossRefGoogle Scholar
- 22.Qi Q, Downer MK, Kilpelainen TO, Taal HR, Barton SJ, Ntalla I, Standl M, Boraska V, Huikari V, Kiefte-de Jong JC, Korner A, Lakka TA, Liu G, Magnusson J, Okuda M, Raitakari O, Richmond R, Scott RA, Bailey ME, Scheuermann K, Holloway JW, Inskip H, Isasi CR, Mossavar-Rahmani Y, Jaddoe VW, Laitinen J, Lindi V, Melen E, Pitsiladis Y, Pitkanen N, Snieder H, Heinrich J, Timpson NJ, Wang T, Yuji H, Zeggini E, Dedoussis GV, Kaplan RC, Wylie-Rosett J, Loos RJ, Hu FB, Qi L (2015) Dietary intake, FTO genetic variants, and adiposity: a combined analysis of over 16,000 children and adolescents. Diabetes 64:2467–2476CrossRefGoogle Scholar
- 23.Claussnitzer M, Dankel SN, Kim KH, Quon G, Meuleman W, Haugen C, Glunk V, Sousa IS, Beaudry JL, Puviindran V, Abdennur NA, Liu J, Svensson PA, Hsu YH, Drucker DJ, Mellgren G, Hui CC, Hauner H, Kellis M (2015) FTO obesity variant circuitry and adipocyte browning in humans. N Engl J Med 373:895–907CrossRefGoogle Scholar
- 29.Rajwani A, Ezzat V, Smith J, Yuldasheva NY, Duncan ER, Gage M, Cubbon RM, Kahn MB, Imrie H, Abbas A, Viswambharan H, Aziz A, Sukumar P, Vidal-Puig A, Sethi JK, Xuan S, Shah AM, Grant PJ, Porter KE, Kearney MT, Wheatcroft SB (2012) Increasing circulating IGFBP1 levels improves insulin sensitivity, promotes nitric oxide production, lowers blood pressure, and protects against atherosclerosis. Diabetes 61:915–924CrossRefGoogle Scholar
- 36.Smith TJ, Rigassio-Radler D, Denmark R, Haley T, Touger-Decker R (2013) Effect of Lactobacillus rhamnosus LGG(R) and Bifidobacterium animalis ssp. lactis BB-12(R) on health-related quality of life in college students affected by upper respiratory infections. Br J Nutr 109:1999–2007CrossRefGoogle Scholar
- 41.Brenseke B, Prater MR, Bahamonde J, Gutierrez JC (2013) Current thoughts on maternal nutrition and fetal programming of the metabolic syndrome. J Pregnancy 2013:368461Google Scholar