Effects of dietary l-methionine supplementation on intestinal integrity and oxidative status in intrauterine growth-retarded weanling piglets
- 360 Downloads
The present study investigated whether dietary methionine supplementation might protect against intrauterine growth retardation (IUGR)-induced damage in the intestine of piglets.
Thirty normal birth weight (NBW) female piglets and sixty same-sex IUGR piglets were weaned at 21 days of postnatal age and fed the control diet (4.0 g methionine per kg of feed, NBW-CON, and IUGR-CON groups) or the methionine-supplemented diet (5.2 g methionine per kg of feed, IUGR-MET group) for 28 days (n = 6).
Piglets in the IUGR-CON group showed decreased average daily feed intake and average daily gain and an increased feed conversion ratio than those in the NBW-CON group. Compared with NBW-CON piglets, IUGR-CON piglets had decreased villus height (VH) and villus height-to-crypt depth ratio in both the jejunum and ileum. In addition, in comparison with the NBW-CON piglets, IUGR increased the concentration of malondialdehyde (MDA) and the index of apoptosis, while it decreased the concentrations of methionine and reduced glutathione (GSH), the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG), and the protein expression of occludin (OCLN) in both the jejunum and ileum. Dietary methionine supplementation decreased the MDA and protein carbonyl concentrations and the apoptotic index, while it increased the VH level, methionine and GSH concentrations, GSH/GSSG ratio, and the OCLN protein expression in the jejunum of IUGR-MET piglets.
Methionine may have beneficial effects in improving intestinal integrity and oxidative status in IUGR weanling piglets.
KeywordsMethionine Intrauterine growth retardation Intestinal damage Oxidative stress Piglets
This work was supported by the National Natural Science Foundation of China (Grant number 31572418) and the Phase II Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The use of animals for this research was approved by the Institutional Animal Care and Use Committee of Nanjing Agricultural University.
- 6.Wang Y, Zhang L, Zhou G, Liao Z, Ahmad H, Liu W, Wang T (2012) Dietary l-arginine supplementation improves the intestinal development through increasing mucosal Akt and mammalian target of rapamycin signals in intra-uterine growth retarded piglets. Br J Nutr 108:1371–1381. doi: 10.1017/S0007114511006763 CrossRefPubMedGoogle Scholar
- 13.Zhang H, Chen Y, Li Y, Yang L, Wang J, Wang T (2014) Medium-chain TAG attenuate hepatic oxidative damage in intra-uterine growth-retarded weanling piglets by improving the metabolic efficiency of the glutathione redox cycle. Br J Nutr 112:876–885. doi: 10.1017/S000711451400155X CrossRefPubMedGoogle Scholar
- 14.Conde-Aguilera JA, Le Floc’h N, Le Huërou-Luron I, Mercier Y, Tesseraud S, Lefaucheur L, van Milgen J (2016) Splanchnic tissues respond differently when piglets are offered a diet 30% deficient in total sulfur amino acid for 10 days. Eur J Nutr 55:2209–2219. doi: 10.1007/s00394-015-1031-x CrossRefPubMedGoogle Scholar
- 15.Bauchart-Thevret C, Stoll B, Chacko S, Burrin DG (2009) Sulfur amino acid deficiency upregulates intestinal methionine cycle activity and suppresses epithelial growth in neonatal pigs. Am J Physiol Endocrinol Metab 296:E1239–E1250. doi: 10.1152/ajpendo.91021.2008 CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Tang Z, Yin Y, Zhang Y, Huang R, Sun Z, Li T, Chu W, Kong X, Li L, Geng M, Tu Q (2009) Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin–lactoferrampin on performance, immune function and intestinal mucosal morphology in piglets weaned at age 21 d. Br J Nutr 101:998–1005. doi: 10.1017/S0007114508055633 CrossRefPubMedGoogle Scholar
- 19.Ferenc K, Pietrzak P, Godlewski MM, Piwowarski J, Kiliańczyk R, Guilloteau P, Zabielski R (2014) Intrauterine growth retarded piglet as a model for humans—studies on the perinatal development of the gut structure and function. Reprod Biol 14:51–60. doi: 10.1016/j.repbio.2014.01.005 CrossRefPubMedGoogle Scholar
- 22.National Research Council (2012) Nutrient requirements of swine, 11th edn. National Academy Press, Washington, DCGoogle Scholar
- 29.Han F, Hu L, Xuan Y, Ding X, Luo Y, Bai S, He S, Zhang K, Che L (2013) Effects of high nutrient intake on the growth performance, intestinal morphology and immune function of neonatal intra-uterine growth-retarded pigs. Br J Nutr 110:1819–1827. doi: 10.1017/S0007114513001232 CrossRefPubMedGoogle Scholar
- 30.Wang W, Degroote J, Van Ginneken C, Van Poucke M, Vergauwen H, Dam TM, Vanrompay D, Peelman LJ, De Smet S, Michiels J (2016) Intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mRNA expression of redox-sensitive genes. FASEB J 30:863–873. doi: 10.1096/fj.15-274779 CrossRefPubMedGoogle Scholar
- 38.Baserga M, Bertolotto C, Maclennan NK, Hsu JL, Pham T, Laksana GS, Lane RH (2004) Uteroplacental insufficiency decreases small intestine growth and alters apoptotic homeostasis in term intrauterine growth retarded rats. Early Hum Dev 79:93–105. doi: 10.1016/j.earlhumdev.2004.04.015 CrossRefPubMedGoogle Scholar
- 44.Alexandre-Gouabau M, Courant F, Le Gall G, Moyon T, Darmaun D, Parnet P, Coupé B, Antignac JP (2011) Offspring metabolomic response to maternal protein restriction in a rat model of intrauterine growth restriction (IUGR). J Proteome Res 10:3292–3302. doi: 10.1021/pr2003193 CrossRefPubMedGoogle Scholar
- 45.MacLennan NK, James SJ, Melnyk S, Piroozi A, Jernigan S, Hsu JL, Janke SM, Pham TD, Lane RH (2004) Uteroplacental insufficiency alters DNA methylation, one-carbon metabolism, and histone acetylation in IUGR rats. Physiol Genomics 18:43–50. doi: 10.1152/physiolgenomics.00042.2004 CrossRefPubMedGoogle Scholar