Comparison of the effects of three different Baccaurea angulata whole fruit juice doses on plasma, aorta and liver MDA levels, antioxidant enzymes and total antioxidant capacity

Abstract

Purpose

Baccaurea angulata (common names: belimbing dayak or belimbing hutan) is a Malaysian underutilized fruit. The preliminary work on B. angulata fruit juice showed that it possesses antioxidant properties. Therefore, further work is needed to confirm the efficacy and proper dosage of B. angulata as a potential natural antioxidant. The present study was thus carried out to compare the effects of three different B. angulata whole fruit (WF) juice doses administered at nutritional doses of 0.50, 1.00 and 1.50 ml/kg/day on plasma, aorta and liver malondialdehyde (MDA) levels, antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase) as well as total antioxidant capacity in rabbits fed high-cholesterol diet.

Methods

Thirty-five male rabbits of New Zealand strain were randomly assigned to seven groups. For 12 weeks, group CH was fed 1% cholesterol diet only; group C1 was fed 1% cholesterol diet and 0.50 ml/kg/day B. angulata WF juice; group C2 was fed 1% cholesterol diet and 1.00 ml/kg/day B. angulata WF juice; group C3 was fed 1% cholesterol diet and 1.50 ml/kg/day B. angulata WF juice; group N was fed standard pellet only; group N1 was fed standard pellet and 0.50 ml/kg/day B. angulata WF juice; and group N2 was fed standard pellet and 1.00 ml/kg/day B. angulata WF juice.

Results

The three doses reduced the formation of MDA and enhanced the expression of endogenous antioxidant enzymes. The highest dose used (1.50 ml/kg/day) was, however, seen as the most potent.

Conclusion

Higher doses of B. angulata juice exerted better antioxidant activity.

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Acknowledgments

The authors are thankful to the Ministry of Science, Technology and Innovation (MOSTI) for funding this project (Project Nos: 06-01-08-SF0111 and 06-01-08-SF0112). The authors are also very grateful to Integrated Centre for Research Animal Care and Use (ICRACU) and Kulliyyah of Allied Health Science, IIUM for financial and technical assistances.

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Correspondence to Muhammad Ibrahim.

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Ibrahim, M., Mikail, M.A., Ahmed, I.A. et al. Comparison of the effects of three different Baccaurea angulata whole fruit juice doses on plasma, aorta and liver MDA levels, antioxidant enzymes and total antioxidant capacity. Eur J Nutr 57, 1817–1828 (2018). https://doi.org/10.1007/s00394-017-1466-3

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Keywords

  • Antioxidant enzymes
  • Baccaurea angulata
  • Doses
  • Malondialdehyde
  • Underutilized fruit