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Effect of GPR84 deletion on obesity and diabetes development in mice fed long chain or medium chain fatty acid rich diets

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Abstract

Purpose

Although there is good evidence showing that diets rich in medium chain fatty acids (MCFAs) have less marked obesogenic and diabetogenic effects than diets rich in long chain fatty acids (LCFAs), the role of the pro-inflammatory, medium chain fatty acid receptor (GPR84) in the aetiology of obesity and glucose intolerance is not well characterised. We set out to determine whether GPR84 expression influences obesity and glucose intolerance susceptibility in MCFA and LCFA rich diet fed mice.

Methods

Wild type (WT) and GPR84 knockout (KO) mice were fed a control, MCFA or LCFA diet, and body mass, heart, liver and epididymal fat mass was assessed, as well as glucose tolerance and adipocyte size.

Results

LCFA diets increased body mass and decreased glucose tolerance in both WT and GPR84 KO animals while MCFA diets had no effect on these parameters. There were no differences in body weight when comparing WT and GPR84 KO mice on the respective diets. Glucose tolerance was also similar in WT and GPR84 KO mice irrespective of diet. Liver mass was increased following LCFA feeding in WT but not GPR84 KO mice. Hepatic triglyceride content was increased in GPR84 KO animals fed MCFA, and myocardial triglyceride content was increased in GPR84 KO animals fed LCFA.

Conclusions

GPR84 deletion had no effects on body weight or glucose tolerance in mice fed either a high MCFA or LCFA diet. GPR84 may influence lipid metabolism, as GPR84 KO mice had smaller livers and increased myocardial triglyceride accumulation when fed LCFA diets, and increased liver triglyceride accumulation in responses to increased dietary MCFAs.

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Acknowledgements

This work was supported in part by National Health and Medical Research Council (Australia) Program Grant 1071659 to MPJ

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Authors and Affiliations

  1. School of Medical Science, Griffith University, Gold Coast Campus, Southport, QLD, Australia

    Eugene Du Toit, Helen Irving-Rodgers, Helen M. Massa, Nicolette Fozzard & Ian R. Peak

  2. Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, QLD, Australia

    Liam Browne, Michael P. Jennings & Ian R. Peak

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  1. Eugene Du Toit
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  2. Liam Browne
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Correspondence to Ian R. Peak.

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Ethical standards

This study was approved by the Griffith University Animal Ethics Committee, under protocol GUAEC/GLY/06/14 and was conducted to comply with the Australian Code for the Care and Use of Animals for Scientific Purposes, 8th edition (2013).

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Du Toit, E., Browne, L., Irving-Rodgers, H. et al. Effect of GPR84 deletion on obesity and diabetes development in mice fed long chain or medium chain fatty acid rich diets. Eur J Nutr 57, 1737–1746 (2018). https://doi.org/10.1007/s00394-017-1456-5

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  • DOI: https://doi.org/10.1007/s00394-017-1456-5

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