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Fructooligosaccharide intake promotes epigenetic changes in the intestinal mucosa in growing and ageing rats

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Abstract

Purpose

The aim of this study was to investigate the relationship between fructooligosaccharide (FOS) intake at different life stages of Wistar rats and its stimulatory effects on intestinal parameters.

Methods

Recently weaned and ageing female rats were divided into growing and ageing treatments, which were fed diets that partially replaced sucrose with FOS for 12 weeks.

Results

Dietary FOS intake induced a significant increase in the numbers of Bifidobacterium and Lactobacillus in growing rats. FOS intake was associated with increased butyric acid levels and a reduced pH of the caecal contents at both ages. Differential gene expression patterns were observed by microarray analysis of growing and ageing animals fed the FOS diet. A total of 133 genes showed detectable changes in expression in the growing rats, while there were only 19 gene expression changes in ageing rats fed with FOS.

Conclusion

These results suggest that dietary FOS intake may be beneficial for some parameters of intestinal health in growing rats.

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Acknowledgements

We acknowledge the Microarray Laboratory at the Brazilian Biosciences National Laboratory (LNBio), CNPEM, Campinas, Brazil for their support in the use of equipment, Fluidics station and Scanner GeneChip. We are grateful to Clariant SA (São Paulo, Brazil) for providing FOS (Orafti® P95). Finally, special thanks are also due to Espaço da Escrita, UNICAMP, Campinas, Brazil who reviewed this manuscript.

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Authors and Affiliations

  1. School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil

    Glaucia Carielo Lima, Vivian Cristine Correa Vieira, Cinthia Baú Betim Cazarin & Mário Roberto Maróstica Junior

  2. Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil

    Rafaela da Rosa Ribeiro, Cibele Lima de Albuquerque & Áureo Tatsumi Yamada

  3. Institute of Chemistry, University of São Paulo, São Carlos, São Paulo, Brazil

    Stanislau Bogusz Junior

  4. Sainte-Justine University Hospital Center, Université de Montreal, Montreal, Canada

    Ramon Oliveira Vidal

  5. Department of Biochemistry, Biological Sciences and Health Center, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Claudia Cardoso Netto

  6. Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil

    Fabio Augusto

  7. Laboratório de Nutrição e Metabolismo–Departamento de Alimentos e Nutrição, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, R. Monteiro Lobato 80, Campinas, SP, 13083-862, Brazil

    Mário Roberto Maróstica Junior

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  1. Glaucia Carielo Lima
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Correspondence to Mário Roberto Maróstica Junior.

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Financial support

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (Grant 2010/05681-8 and 2010/16752-3), CAPES, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 300533/2013-6) also provided financial support. Fundação de Amparo à Pesquisa do Estado de São Paulo had no role in the design, analysis or writing of this article.

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Lima, G.C., Vieira, V.C.C., Cazarin, C.B.B. et al. Fructooligosaccharide intake promotes epigenetic changes in the intestinal mucosa in growing and ageing rats. Eur J Nutr 57, 1499–1510 (2018). https://doi.org/10.1007/s00394-017-1435-x

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