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Effects of Lentinula edodes consumption on biochemical, hematologic and oxidative stress parameters in rats receiving high-fat diet

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Abstract

Purpose

Functional foods can prevent/reduce the risks related to obesity. Lentinula edodes is a highly nutritious mushroom rich in protein, vitamins and minerals. Some studies have demonstrated the hypocholesterolemic effects from L. edodes in high doses, which does not represent the consumption in humans. We evaluated ingestion of a realistic dose of L. edodes associated with a high-fat diet (HFD) on hematologic, biochemical and oxidative stress parameters.

Methods

Eighteen male Wistar rats were divided into three groups: control (normal diet); HFD; and HFD + L. edodes (100 mg/kg/day). After 30 days, blood was collected. Biochemical and hematologic parameters were analyzed, as well as oxidative stress biomarkers.

Results

The HFD increased levels of total cholesterol and triglycerides. Lentinula edodes reduced these parameters significantly to concentrations found in the control group. The HFD increased levels of alanine transaminase and aspartate transaminase (markers of liver damage). Lentinula edodes returned the levels of these enzymes to normal levels and normalized serum levels of urea (which were also increased owing to consumption of the HFD). Lentinula edodes reduced levels of urea and glucose. Lipid peroxidation was increased in rats receiving the HFD, and L. edodes reduced malondialdehyde levels, thereby preventing oxidation of fatty acids.

Conclusions

Lentinula edodes was shown to have hypolipidemic, hypoglycemic, hepatoprotective and renoprotective features in doses that are suitable for humans.

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Acknowledgments

The authors are grateful for financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-2013/05765-5) and Financiadora de Estudos e Projetos (FINEP-01.10.0659.00) and the Programa de Suporte à Pós-Graduação de Instituições de Ensino Particulares (PROSUP/CAPES). We also thank Thaisa Borim Pickler for technical support.

Funding sources

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-2013/05765-5) and Financiadora de Estudos e Projetos (FINEP-01.10.0659.00).

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Authors and Affiliations

  1. Laboratory of Toxicological Research, Rodovia Raposo Tavares, University of Sorocaba, Km 92.5, Sorocaba, São Paulo State, 18023-000, Brazil

    Sara Rosicler Vieira Spim, Bruna Giovanna Corrêa Chrispim de Oliveira, Fernanda Gomes Leite, Marli Gerenutti & Denise Grotto

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  1. Sara Rosicler Vieira Spim
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  2. Bruna Giovanna Corrêa Chrispim de Oliveira
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Correspondence to Denise Grotto.

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Spim, S.R.V., de Oliveira, B.G.C.C., Leite, F.G. et al. Effects of Lentinula edodes consumption on biochemical, hematologic and oxidative stress parameters in rats receiving high-fat diet. Eur J Nutr 56, 2255–2264 (2017). https://doi.org/10.1007/s00394-016-1266-1

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