Effects of proanthocyanidin on oxidative stress biomarkers and adipokines in army cadets: a placebo-controlled, double-blind study
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The relatively recent advent of polyphenol supplement for exercise studies has been tested in a variety of forms and doses. However, the dose–response on adipokines and oxidative stress biomarker effect remains unknown. The aim of the present study was to assess the effect of intense, long-duration (48-h) exercise, and a single dose of proanthocyanidin, on plasma leptin, adiponectin, and electronegative low-density lipoprotein (LDL(−)) concentrations.
Fifty-four healthy male army cadets (22 ± 2 years) participated in a double-blind, randomized, placebo-controlled study and were distributed between control (CG; n = 27) and supplemented groups (SG; n = 27). Immediately before the start of the exercise, both CG and SG groups received a capsule containing starch (200 mg) or proanthocyanidin (dry Vitis vinifera extract, 200 mg), respectively. Following a 12-h fasting period, the plasma adiponectin, leptin, and LDL(−) concentrations were measured prior to the start of the exercise after 24 and 48 h of military training, and after 24 h of rest. The effects of the proanthocyanidin (supplement), exercise (time), and their interaction were investigated using factorial two-way ANOVA.
Plasma leptin concentration was only influenced by exercise (p = 0.001). Plasma adiponectin concentration was influenced by exercise (p = 0.037), and by the exercise x supplement interaction (p = 0.033). LDL(−) was influenced by the supplement (p = 0.001), exercise (p = 0.001), and their interaction (p = 0.001).
A single dose of proanthocyanidin (200 mg) was able to reduce LDL(−) concentration and increase plasma adiponectin concentration after 24 h of rest in SG group, indicating its potential protective action.
KeywordsLow-density lipoprotein Adiponectin Leptin Exercise Polyphenols Army cadets
The authors would like to thank all of the army cadets that participated in this study and Mario Vila Pitaluga Filho for his assistance in the organization of the collection data. This research was supported by Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ).
Koury JC, Passos MC, Oliveira CF, Gonçalves M, and Daleprane JB participated in the conception and design of the study; generation, collection, assembly, analysis, and interpretation of data; and drafting, revision, and final approval of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Manach C, Scalbert A, Morand C, Remesy C, Jimenez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79:727–747Google Scholar
- 5.Coyle EF (2000) Physical activity as a metabolic stressor. Am J Clin Nutr 72(2 suppl):512S–520SGoogle Scholar
- 6.Drenawatz C, Hand GA, Sagner M, Shook RP, Burgers S, Blair SN (2015) The prospective association between different types of exercise and body composition. Med Sci Sports Exerc. May 12 [Epub ahead a print]Google Scholar
- 13.Cho SY, Park PJ, Shin HJ, Kim YK, Shin DW, Shin ES, Lee HH, Lee BG, Baik JH, Lee TR (2007) Catechin suppresses expression of Kruppel-like factor and increases expression and secretion of adiponectin protein in 3T3-L1 cells. Am J Physiol Endocrinol Metab 292:E1166–E1172. doi: 10.1152/ajpendo.00436.2006 CrossRefGoogle Scholar
- 21.Loke WM, Hodgson JM, Proudfoot JM, McKinley AJ, Puddey IB, Loke KD (2008) Pure dietary flavonoids quercetin and (–)-epicatechin augment nitric oxide products and reduce endothelin-1 acutely in healthy men. Am J Clin Nutr 88:1018–1025Google Scholar
- 22.Mennen LI, Walker R, Bennetau-Pelissero C, Scalbert A (2005) Risks and safety of polyphenol consumption. Am J Clin Nutr 81(1 Suppl):326S–329SGoogle Scholar
- 23.World Health Organization (2004) Fruit and vegetables for health. Report of a joint FAO/WHO workshop, 1–3 September 2004, Kobe, JapanGoogle Scholar
- 24.Jaime PC, Figueiredo ICR, de Moura EC, Malta DC (2009) Factors associated with fruit and vegetable consumption in Brazil, 2006. Rev Saúde Pública 43(Suppl 2):1–8Google Scholar
- 25.World Health Organization (1995) Physical status: the use and interpretation of anthropometry. WHO technical report series no. 854. Geneva, SwitzerlandGoogle Scholar
- 30.Sacher RA, McPherson RA (2002) Hematologia. In: Widman’s Clinical Interpretation of laboratory tests, 11th edn. F.A. Davis Company, Philadelphia, pp 431–441Google Scholar
- 31.Lohman TG (1997) Body composition assessment: body fat standards and methods in the field of exercise and sports medicine. ACSM’s Health Fit J 1:30–33Google Scholar
- 32.Baker-Fulco CJ, Bathalon GP, Bovill ME, Lieberman HR (2001) Military dietary reference intakes: rationale for tabled values US army medical research and materiel command. United States Army Research Institute of Environmental Medicine, Natick, Massachusetts. Technical Report No. T02-23Google Scholar
- 35.Jürimäe J, Rämson R, Mäestu J, Jürimäe T, Arciero PJ, Braun WA, LeMura LM, Von Duvillard SP (2011) Interactions between adipose, bone, and muscle tissue markers during acute negative energy balance in male rowers. J Sports Med Phys Fit 51:347–354Google Scholar
- 38.Gonçalves MC, Passos MC, Beltrame JD, Koury JC (2014) Is it possible to identify underlying cardiovascular risk in young trained military? J Sports Med Phys Fit. Nov 4 [Epub ahead a print]Google Scholar