Citrulline malate (CM) is a nonessential amino acid that increases exercise performance in males. However, based on physiological differences between genders, these results cannot be extrapolated to females. Therefore, the purpose of this investigation was to evaluate effects of acute CM supplementation on upper- and lower-body weightlifting performance in resistance-trained females.
Fifteen females (23 ± 3 years) completed two randomized, double-blind trials consuming either CM (8 g dextrose + 8 g CM) or a placebo (8 g dextrose). One hour after supplement consumption, participants performed six sets each of upper- (i.e., bench press) and lower-body (i.e., leg press) exercises to failure at 80 % of previously established one-repetition maximum. Immediately after each set, repetitions completed, heart rate and rating of perceived exertion (RPE) were recorded.
Repeated-measures analysis of variance indicated that subjects completed significantly (p = .045) more repetitions throughout upper-body exercise when consuming CM versus placebo (34.1 ± 5.7 vs. 32.9 ± 6.0, respectively). When consuming CM, similar significant (p = .03) improvements in total repetitions completed were observed for lower-body exercise (66.7 ± 30.5 vs. 55.13 ± 20.64, respectively). Overall RPE score was significantly lower (p = .02) in upper-body exercise when subjects consumed CM versus placebo (7.9 ± 0.3 and 8.6 ± 0.2, respectively). The supplement consumed exhibited no significant effects on heart rate at any time point.
Acute CM supplementation in females increased upper- and lower-body resistance exercise performance and decreased RPE during upper-body exercise. These data indicate that athletes competing in sports with muscular endurance-based requirements may potentially improve performance by acutely supplementing CM.
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Silver MD (2001) Use of ergogenic aids by athletes. J Am Acad Orthop Surg 9:61–70
Eichner ER (1997) Ergogenic aids: what athletes are using and why. Phys Sports Med 25:70–83
Coleman E (1998) Ergogenic aids for athletes. Clin Nutr Insight 24:1–4
Tokish JM, Kocher MS, Hawkins RJ (2004) Ergogenic aids: a review of basic science, performance, side effects, and status in sports. Am J Sports Med 32:1543–1553
Calfee R, Fadale P (2006) Popular ergogenic drugs and supplements in young athletes. Pediatrics 117:577–589
Nissen SL, Sharp RL (2003) Effect of dietary supplements on lean mass and strength gains with resistance exercise: a meta-analysis. J Appl Physiol 94:651–659
Cribb PJ, Hayes A (2006) Effects of supplement-timing and resistance exercise on skeletal muscle hypertrophy. Med Sci Sports Exerc 38:1918–1925
Beery AK, Zucker I (2011) Sex bias in neuroscience and biomedical research. Neurosci Biobehav Rev 35:565–572
Soldin OP, Mattison DR (2009) Sex differences in pharmacokinetics and pharmacodynamics. Clin Pharmacokinet 48:143–157
United States Department of Commerce (2011) Age and sex composition: 2010, vol 1. U S Census Bureau, pp 1–16
Herbold NH, Visconti BK, Frates S, Bandini L (2004) Traditional and nontraditional supplement use by collegiate female varsity athletes. Int J Sport Nutr Exerc Metab 14:583–593
Bescos R, Sureda A, Tur JA, Pons A (2012) The effect of nitric-oxide-related supplements on human performance. Sports Med 42:99–117
Petrovic V, Buzadzic B, Korac A, Vasilijevic A, Jankovic A, Micunovic K, Korac B (2008) Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role of l-arginine/NO-producing pathway. J Exp Biol 211:114–120
Wax B, Kavazis AN, Luckett W (2015) Effects of supplemental citrulline-malate ingestion on blood lactate, cardiovascular dynamics, and resistance exercise performance in trained males. J Diet Suppl 13(3):269–282
Wax B, Kavazis AN, Weldon K, Sperlak J (2014) Effects of supplemental citrulline malate ingestion during repeated bouts of lower-body exercise in advanced weight lifters. J Strength Cond Res 29:786–792
Perez-Guisado J, Jakeman PM (2010) Citrulline malate enhances athletic anaerobic performance and relieves muscle soreness. J Strength Cond Res 24:1215–1222
Rouge C, Des Robert C, Robins A, Le Bacquer O, Volteau C, De La Cochetiere MF, Darmaun D (2007) Manipulation of citrulline availability in humans. Am J Physiol Gastrointest Liver Physiol 293:1061–1067
Cutrufello PT, Gadomski SJ, Zavorsky GS (2015) The effect of l-citrulline and watermelon juice supplementation on anaerobic and aerobic exercise performance. J Sports Sci 33:1459–1466
Blair ML (2007) Sex-based differences in physiology: what should we teach in the medical curriculum? Adv Physiol Educ 31:23–25
Stachenfeld NS, Taylor HS (2014) Challenges and methodology for testing young healthy women in physiological studies. Am J Physiol Endocrinol Metab 306:849–853
Guetta V, Quyyumi AA, Prasad A, Panza JA, Waclawiw M, Cannon RO (1997) The role of nitric oxide in coronary vascular effects of estrogen in postmenopausal women. Circulation 96:2795–2801
Mendelsohn ME, Karas RH (1994) Estrogen and the blood vessel wall. Curr Opin Cardiol 9:619–626
American College of Sports Medicine (2013) ACSM’s guidelines for exercise testing and prescription. Lippincott Williams & Wilkins, Philadelphia
Jurkowski J, Jones NL, Toews CJ, Sutton JR (1981) Effects of menstrual cycle on blood lactate, O2 delivery, and performance during exercise. J Appl Physiol 51:1493–1499
Glenn J, Smith K, Moyen N, Binns A, Gray M (2015) Effects of acute beta-alanine supplementation on anaerobic performance in trained female cyclists. J Nutr Sci Vitaminol 61:161–166
Armstrong LE, Maresh CM, Keith NR, Elliott TA, Vanheest JL, Scheett TP, Stoppani J, Judelson DA, De Souza MJ (2005) Heat acclimation and physical training adaptations of young women using different contraceptive hormones. Am J Physiol Endocrinol Metab 288:868–875
Glenn J, Gray M, Vincenzo J (2014) Differences in regional adiposity, bone mineral density, and physical exercise participation based on exercise self-efficacy among senior adults. J Sports Med Phys Fit 55(10):1166–1173
Baechle TR, Earle RW (2008) Essentials of strength training and conditioning. Human Kinetics, Champaign
Robertson RJ, Goss FL, Rutkowski J, Lenz B, Dixon C, Timmer J, Frazee K, Dube J, Andreacci J (2003) Concurrent validation of the OMNI perceived exertion scale for resistance exercise. Med Sci Sports Exerc 35:333–341
Lagally KM, Robertson RJ (2006) Construct validity of the OMNI resistance exercise scale. J Strength Cond Res 20:252–256
McGee KJ, Burkett LN (2003) The National Football League combine: a reliable predictor of draft status? J Strength Cond Res 17:6–11
Sierer SP, Battaglini CL, Mihalik JP, Shields EW, Tomasini NT (2008) The National Football League combine: performance differences between drafted and nondrafted players entering the 2004 and 2005 drafts. J Strength Cond Res 22:6–12
McGill SM, Andersen JT, Horne AD (2012) Predicting performance and injury resilience from movement quality and fitness scores in a basketball team over 2 years. J Strength Cond Res 26:1731–1739
Gallagher D, Heymsfield SB (1998) Muscle distribution: variations with body weight, gender, and age. Appl Radiat Isot 49:733–734
Janssen I, Heymsfield SB, Wang ZM, Ross R (2000) Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr. J Appl Physiol 89:81–88
Noakes TD (2004) Linear relationship between the perception of effort and the duration of constant load exercise that remains. J Appl Physiol 96:1571–1573
Bloomer RJ, Farney TM, Trepanowski JF, McCarthy CG, Canale RE, Schilling BK (2010) Comparison of pre-workout nitric oxide stimulating dietary supplements on skeletal muscle oxygen saturation, blood nitrate/nitrite, lipid peroxidation, and upper body exercise performance in resistance trained men. J Int Soc Sports Nutr 7:1–15
Hartman WJ, Torre PM, Prior RL (1994) Dietary citrulline but not ornithine counteracts dietary arginine deficiency in rats by increasing splanchnic release of citrulline. J Nutr 124:1950–1960
Bendahan D, Mattei JP, Ghattas B, Confort-Gouny S, Le Guern ME, Cozzone PJ (2002) Citrulline/malate promotes aerobic energy production in human exercising muscle. Br J Sports Med 36:282–289
Shen W, Xu X, Ochoa M, Zhao G, Wolin MS, Hintze TH (1994) Role of nitric oxide in the regulation of oxygen consumption in conscious dogs. Circ Res 75:1086–1095
Walker JB (1979) Creatine: biosynthesis, regulation, and function. Adv Enzymol Relat Areas Mol Biol 50:177–242
Paddon-Jones D, Borsheim E, Wolfe RR (2004) Potential ergogenic effects of arginine and creatine supplementation. J Nutr 134:2888–2894
Little JP, Forbes SC, Candow DG, Cornish SM, Chilibeck PD (2008) Creatine, arginine α-ketoglutarate, amino acids, and medium-chain triglycerides and endurance and performance. Int J Sport Nutr Exerc Metab 18:493–508
Funding for this investigation was provided by the Statewide Undergraduate Research Fellowship. The authors would like to thank Jarrion Lawson for his help with the dietary analysis and data collection portions of this project. We would also like to thank Shari Witherspoon for her assistance during the process of ordering and acquiring the necessary materials required throughout this investigation.
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Glenn, J.M., Gray, M., Wethington, L.N. et al. Acute citrulline malate supplementation improves upper- and lower-body submaximal weightlifting exercise performance in resistance-trained females. Eur J Nutr 56, 775–784 (2017). https://doi.org/10.1007/s00394-015-1124-6
- Ergogenic aid
- Sports nutrition
- Amino acids
- Resistance exercise
- Nitric oxide