Methylation patterns of Vegfb promoter are associated with gene and protein expression levels: the effects of dietary fatty acids
We have investigated the epigenetic regulation by dietary fatty acids of Vegfb levels in rats’ white adipose tissue and 3T3-L1 cells.
A group of rats were assigned to three diets, each one with a different composition of saturated, monounsaturated and polyunsaturated fatty acids. Samples of white adipose tissues were taken for the methylation and expression studies. Additionally, 3T3-L1 cells were treated with palmitic, oleic, and linoleic fatty acids. After treatment, cells were harvested and genetic material was extracted for the analysis of Vegfb levels.
We report evidence of changes in the methylation levels of the CpG island at the Vegfb promoter and in the Vegfb expression levels in vivo and in vitro by dietary fatty acid, with the main contribution of the linoleic fatty acid. Vegfb promoter methylation levels were closely related to the Vegfb gene expression.
According to our results, the regulation of Vegfb gene expression by dietary fatty acids may be mediated, at least in part, by epigenetic modifications on Vegfb promoter methylation. Considering the deep association between angiogenesis and tissue growth, we suggest the nutriepigenetic regulation of Vegfb as a key target in the control of the adipose tissue expansion.
KeywordsAdipose tissue Dietary fatty acids Gene expression Nutriepigenetic regulation Vegfb
We gratefully acknowledge the help of Ron Askew-Reeves for the English corrections of this manuscript. The research group belong to the “Centros de Investigación Biomédica en Red” (CIBERDEM CB07/08/0019) of the Instituto de Salud Carlos III. We acknowledge the very useful assistance provided by the Plataforma de Genomica ECAI of the IBIMA Institute. This study was undertaken with finance from the Fondo de Investigación Sanitaria of the Instituto de Salud Carlos III and the Fondo Europeo de Desarrollo Regional FEDER (PI11/00880 and PI12/01293), Metabolism and Nutrition Network (RCMYN C03-08) and Junta de Andalucía (02/03, 125/02, 115/02).
EG-E, GR-M and FS designed research; EG-E, RM, AL, SG-S, GMM-N, FR-P and JMG-Z performed the experiments; EG-E, NC, SM, FS, EG-F contributed to discussion; EG-E and GR-M analyzed data and wrote the paper. All authors have read and reviewed the manuscript.
Compliance with ethical standards
Conflict of interest
Authors have nothing to disclose.
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