European Journal of Nutrition

, Volume 56, Issue 2, pp 693–704 | Cite as

Relation between neonatal malnutrition and gene expression: inflammasome function in infections caused by Candida Albicans

  • Thacianna Barreto Da Costa
  • Natália Gomes De Morais
  • Joana Maria Bezerra De Lira
  • Thays Miranda De Almeida
  • Suênia Da Cunha Gonçalves-De-Albuquerque
  • Valéria Rêgo Alves Pereira
  • Milena De Paiva Cavalcanti
  • Célia Maria Machado Barbosa De Castro
Original Contribution
  • 196 Downloads

Abstract

Purpose

To investigate the effects of neonatal malnutrition followed by nutritional replacement on the signaling mechanisms developed by the inflammasome complex by analyzing the expression of the targeted TLR2, TLR4, NLRP3, caspase-1 and release of IL-1β and IL-18 by alveolar macrophages infected in vitro with Candida albicans.

Methods

Male Wistar rats (n = 24), 90–120 days, were suckled by mothers whose diet during lactation contained 17 % protein in the nourish group and 8 % protein in the malnourished group. After weaning, both groups were fed a normal protein diet. Macrophages were obtained after tracheostomy, through the collection of bronchoalveolar lavage fluid. The quantification of the expression levels of targets (TLR2, TLR4, NLRP3 and caspase-1) was performed by real-time RT-PCR. Production of cytokines was performed by ELISA.

Results

The malnourished animals during lactation showed reduced body weight from the fifth day of life, remaining until adulthood. Further, the model applied malnutrition induced a lower expression of TLR4 and caspase-1. The quantification of the TLR2 and NLRP3, as well as the release of IL-1β and IL-18, was not different between groups of animals nourished and malnourished. The system challenged with Candida albicans showed high expression levels of all targets in the study.

Conclusions

The tests demonstrate nutritional restriction during critical periods of development, although nutritional supplementation may compromise defense patterns in adulthood in a timely manner, preserving distinct signaling mechanism, so that the individual does not become widely vulnerable to infections by opportunistic pathogens.

Keywords

Neonatal malnutrition Programming Macrophage Candida albicans Toll-like receptors NOD-like receptors 

Notes

Acknowledgments

The authors wish to thank the Keizo Asami Immunopathology Laboratory of the Federal University of Pernambuco, Recife, Brazil, and Department of Immunology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Campus UFPE, Recife, Brazil, for the structure and equipment provided for the development of this research.

Funding

The study was supported by National Counsel of Technological and Scientific Development (CNPq), Brazil, and partially funded by Dean of Research and Graduate Studies, Federal University of Pernambuco (PROPESQ/UFPE), Brazil.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical standards

This study was approved by Ethics Committee on Animal Experimentation of the Center for Biological Sciences, Federal University of Pernambuco (CEEA-UFPE) (Pernambuco, Brazil, Protocol 23076.053096/2011-91 ). The manuscript does not contain clinical studies or patient data.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Thacianna Barreto Da Costa
    • 1
    • 2
  • Natália Gomes De Morais
    • 1
    • 2
  • Joana Maria Bezerra De Lira
    • 2
  • Thays Miranda De Almeida
    • 3
  • Suênia Da Cunha Gonçalves-De-Albuquerque
    • 3
  • Valéria Rêgo Alves Pereira
    • 3
  • Milena De Paiva Cavalcanti
    • 3
  • Célia Maria Machado Barbosa De Castro
    • 1
    • 2
  1. 1.Department of Tropical MedicineFederal University of PernambucoRecifeBrazil
  2. 2.Keizo Asami Laboratory of ImmunopathologyFederal University of PernambucoRecifeBrazil
  3. 3.Department of Immunology, Aggeu Magalhães Research CenterOswaldo Cruz FoundationRecifeBrazil

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