European Journal of Nutrition

, Volume 56, Issue 2, pp 621–634 | Cite as

Vitamin D3 supplementation does not modify cardiovascular risk profile of adults with inadequate vitamin D status

  • Eric Seibert
  • Ulrike Lehmann
  • Annett Riedel
  • Christof Ulrich
  • Frank Hirche
  • Corinna Brandsch
  • Jutta Dierkes
  • Matthias Girndt
  • Gabriele I. Stangl
Original Contribution



The Nutrition Societies in Germany, Austria, and Switzerland recommend a daily intake of 20 µg vitamin D3 for adults when endogenous synthesis is absent. The current study aimed to elucidate whether this vitamin D3 dose impacts cardiovascular risk markers of adults during the winter months.


The study was conducted in Halle (Saale), Germany (51o northern latitude) as a placebo-controlled, double-blinded, randomised trial (from January to April). A total of 105 apparently healthy subjects (male and female, 20–71 years old) were included. Subjects were randomly allocated to two groups. One group received a daily 20-µg vitamin D3 dose (n = 54), and the other group received a placebo (n = 51) for 12 weeks. Outcome measures included blood pressure, heart rate, concentrations of renin, aldosterone, serum lipids and vascular calcification markers, and haematologic variables such as pro-inflammatory monocytes.


Blood pressure and systemic cardiovascular risk markers remained unchanged by vitamin D3 supplementation, although serum 25-hydroxyvitamin D3 increased from 38 ± 14 to 73 ± 16 nmol/L at week 12. The placebo and vitamin D groups did not differ in their final cardiovascular risk profile.


Daily supplementation of 20 µg vitamin D3 during winter is unlikely to change cardiovascular risk profile.


Vitamin D3 Supplementation Cardiovascular risk Monocyte subsets Adults 



Body mass index


Bone morphogenetic protein 2


Blood pressure




Coefficient of variance


Dickkopf-related protein 1


Fluorescence-activated cell sorting


High-density lipoprotein


Interleukin 10


Low-density lipoprotein


Mean corpuscular haemoglobin


Mean corpuscular haemoglobin concentration


Mean corpuscular volume


Major histocompatibility complex




Receptor activator of nuclear factor kappa-B ligand


Randomised controlled trial


Large ribosomal protein


Rate pressure product


Runt-related transcription factor 2


Tumour necrosis factor α


Toll-like receptor 2


Vitamin D receptor


25-Hydroxyvitamin D



This study was supported by a Grant from The Federal Ministry of Education and Research of Germany (01EA1323A). We thank B. Heinze and M. Hajri for their excellent technical assistance and record keeping. We thank Dr. B. Ludwig-Kraus and colleagues for determination of differential blood counts at the central laboratory unit of the University Hospital Halle (UKH, Ernst-Grube-Str. 40, 06120 Halle/Saale, Germany).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

394_2015_1106_MOESM1_ESM.pdf (105 kb)
Supplementary material 1 (PDF 104 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Eric Seibert
    • 1
  • Ulrike Lehmann
    • 2
  • Annett Riedel
    • 2
  • Christof Ulrich
    • 1
  • Frank Hirche
    • 2
  • Corinna Brandsch
    • 2
  • Jutta Dierkes
    • 3
  • Matthias Girndt
    • 1
  • Gabriele I. Stangl
    • 2
  1. 1.Department of Internal Medicine IIMartin Luther University Halle-WittenbergHalle/SaaleGermany
  2. 2.Institute of Agricultural and Nutritional SciencesMartin Luther University Halle-WittenbergHalle/SaaleGermany
  3. 3.Department of Clinical MedicineUniversity of BergenBergenNorway

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