European Journal of Nutrition

, Volume 55, Issue 2, pp 845–854 | Cite as

Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice

  • Cheng-chin Hsu
  • Hui-ting Yang
  • Jing-jing Ho
  • Mei-chin Yin
  • Jen-ying Hsu
Original Contribution



The anti-glycative and anti-oxidative effects from Houttuynia cordata leaves aqueous extract (HCAE) in heart and kidney of diabetic mice were examined.


HCAE, at 1 or 2 %, was supplied in drinking water for 8 weeks. Plasma glucose and blood urea nitrogen (BUN) levels and creatine phosphokinase (CPK) activity were measured. The production of oxidative and inflammatory factors was determined. Activity and protein expression of associated enzymes or regulators were analyzed.


HCAE intake at both doses lowered plasma glucose and BUN levels, and CPK activity and also restored creatinine clearance rate in diabetic mice. HCAE intake, only at 2 %, retained plasma insulin levels (P < 0.05). HCAE reduced reactive oxygen species, protein carbonyl, interleukin-6, tumor necrosis factor-alpha, N ε -(carboxymethyl)-lysine, pentosidine and fructose levels, and reserved glutathione content in heart and kidney of diabetic mice (P < 0.05). Diabetes enhanced aldose reductase (AR) activity and protein expression in heart and kidney (P < 0.05). HCAE intake at both doses decreased renal AR activity and protein expression, but only at 2 % lowered cardiac AR activity and protein expression (P < 0.05). Diabetes increased protein expression of RAGE, p47phox and gp91phox, nuclear factor kappa-B (NF-κB) p50, NF-κB p65 and mitogen-activated protein kinase in heart and kidney (P < 0.05). HCAE intake only at 2 % limited RAGE expression, but at 1 and 2 % downregulated p47phox, NF-κB p65 and p-p38 expression in these organs (P < 0.05).


These findings suggest that Houttuynia cordata leaves aqueous extract could ameliorate cardiac and renal injury under diabetic condition.


Houttuynia cordata Diabetes Glycation Polyol pathway NADPH oxidase 



Advanced glycation endproducts


Aldose reductase


Blood urea nitrogen


Creatinine clearance rate


N ε-(carboxymethyl)-lysine


Creatine phosphokinase




2′,7′-Dichlorofluorescein diacetate




Glyceraldehyde-3-phosphate dehydrogenase




Houttuynia cordata leaves aqueous extract




Mitogen-activated protein kinase


Nuclear factor kappa-B


Relative fluorescence unit


Reactive oxygen species


Tumor necrosis factor-alpha



This study was partially supported by a Grant from Ministry of Science and Technology, Taipei City, Taiwan (NSC 102-2313-B-039-002-MY3).

Compliance with ethical standards

Conflict of interest

There was no conflict of interest regarding this manuscript.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Cheng-chin Hsu
    • 1
    • 5
  • Hui-ting Yang
    • 2
  • Jing-jing Ho
    • 2
  • Mei-chin Yin
    • 2
    • 3
  • Jen-ying Hsu
    • 4
  1. 1.School of NutritionChung Shan Medical UniversityTaichung CityTaiwan
  2. 2.Department of NutritionChina Medical UniversityTaichung CityTaiwan
  3. 3.Department of Health and Nutrition BiotechnologyAsia UniversityTaichung CityTaiwan
  4. 4.School of PharmacyChina Medical UniversityTaichung CityTaiwan
  5. 5.Department of NutritionChung Shan Medical University HospitalTaichung CityTaiwan

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