European Journal of Nutrition

, Volume 55, Issue 2, pp 845–854 | Cite as

Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice

  • Cheng-chin Hsu
  • Hui-ting Yang
  • Jing-jing Ho
  • Mei-chin Yin
  • Jen-ying Hsu
Original Contribution

Abstract

Purpose

The anti-glycative and anti-oxidative effects from Houttuynia cordata leaves aqueous extract (HCAE) in heart and kidney of diabetic mice were examined.

Methods

HCAE, at 1 or 2 %, was supplied in drinking water for 8 weeks. Plasma glucose and blood urea nitrogen (BUN) levels and creatine phosphokinase (CPK) activity were measured. The production of oxidative and inflammatory factors was determined. Activity and protein expression of associated enzymes or regulators were analyzed.

Results

HCAE intake at both doses lowered plasma glucose and BUN levels, and CPK activity and also restored creatinine clearance rate in diabetic mice. HCAE intake, only at 2 %, retained plasma insulin levels (P < 0.05). HCAE reduced reactive oxygen species, protein carbonyl, interleukin-6, tumor necrosis factor-alpha, N ε -(carboxymethyl)-lysine, pentosidine and fructose levels, and reserved glutathione content in heart and kidney of diabetic mice (P < 0.05). Diabetes enhanced aldose reductase (AR) activity and protein expression in heart and kidney (P < 0.05). HCAE intake at both doses decreased renal AR activity and protein expression, but only at 2 % lowered cardiac AR activity and protein expression (P < 0.05). Diabetes increased protein expression of RAGE, p47phox and gp91phox, nuclear factor kappa-B (NF-κB) p50, NF-κB p65 and mitogen-activated protein kinase in heart and kidney (P < 0.05). HCAE intake only at 2 % limited RAGE expression, but at 1 and 2 % downregulated p47phox, NF-κB p65 and p-p38 expression in these organs (P < 0.05).

Conclusions

These findings suggest that Houttuynia cordata leaves aqueous extract could ameliorate cardiac and renal injury under diabetic condition.

Keywords

Houttuynia cordata Diabetes Glycation Polyol pathway NADPH oxidase 

Abbreviations

AGEs

Advanced glycation endproducts

AR

Aldose reductase

BUN

Blood urea nitrogen

CCr

Creatinine clearance rate

CML

N ε-(carboxymethyl)-lysine

CPK

Creatine phosphokinase

Cr

Creatinine

DCFH-DA

2′,7′-Dichlorofluorescein diacetate

DNP

Dinitrophenylhydrazine

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GSH

Glutathione

HCAE

Houttuynia cordata leaves aqueous extract

IL

Interleukin

MAPK

Mitogen-activated protein kinase

NF-κB

Nuclear factor kappa-B

RFU

Relative fluorescence unit

ROS

Reactive oxygen species

TNF-alpha

Tumor necrosis factor-alpha

Notes

Acknowledgments

This study was partially supported by a Grant from Ministry of Science and Technology, Taipei City, Taiwan (NSC 102-2313-B-039-002-MY3).

Compliance with ethical standards

Conflict of interest

There was no conflict of interest regarding this manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Cheng-chin Hsu
    • 1
    • 5
  • Hui-ting Yang
    • 2
  • Jing-jing Ho
    • 2
  • Mei-chin Yin
    • 2
    • 3
  • Jen-ying Hsu
    • 4
  1. 1.School of NutritionChung Shan Medical UniversityTaichung CityTaiwan
  2. 2.Department of NutritionChina Medical UniversityTaichung CityTaiwan
  3. 3.Department of Health and Nutrition BiotechnologyAsia UniversityTaichung CityTaiwan
  4. 4.School of PharmacyChina Medical UniversityTaichung CityTaiwan
  5. 5.Department of NutritionChung Shan Medical University HospitalTaichung CityTaiwan

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