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Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells

Abstract

Purpose

Pomegranate fruit, Punica granatum L. (Punicaceae), and its constituents have been shown to inhibit inflammation. In this study, we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE2 production in IL-1β-stimulated SK-N-SH cells.

Methods

An enzyme immunoassay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1β-stimulated SK-N-SH cells. Expression of COX-2, phospho-IκB, and phospho-IKK proteins was evaluated, while NF-κB reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of PWE on NF-κB transactivation. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA.

Results

PWE (25–200 μg/ml) dose dependently reduced COX-2-dependent PGE2 production in SK-N-SH cells stimulated with IL-1β. Phosphorylation of IκB and IKK was significantly (p < 0.001) inhibited by PWE (50–200 μg/ml). Our studies also show that PWE (50–200 μg/ml) significantly (p < 0.01) inhibited NF-κB transactivation in TNFα-stimulated HEK293 cells. Furthermore, PWE inhibited BACE-1 and Aβ expression in SK-N-SH cells treated with IL-1β.

Conclusions

Taken together, our study demonstrates that pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer’s disease.

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Acknowledgments

This study was carried out in part with funding by the Alexander von Humboldt Foundation to Dr. Olumayokun Olajide. We wish to thank Mr. Oluwatodimu Sam-Dahunsi for assisting with freeze-drying of pomegranate juice.

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Correspondence to Olumayokun Olajide.

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Velagapudi, R., Baco, G., Khela, S. et al. Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells. Eur J Nutr 55, 1653–1660 (2016). https://doi.org/10.1007/s00394-015-0984-0

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  • DOI: https://doi.org/10.1007/s00394-015-0984-0

Keywords

  • Pomegranate
  • Neuroinflammation
  • Amyloidogenesis
  • Neurons
  • Interleukin-1β