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Gene expression profiling to investigate tyrosol-induced lifespan extension in Caenorhabditis elegans

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Abstract

Purpose

We have previously reported that tyrosol (TYR) promotes lifespan extension in the nematode Caenorhabditis elegans, also inducing a stronger resistance to thermal and oxidative stress in vivo. In this study, we performed a whole-genome DNA microarray in order to narrow down the search for candidate genes or signaling pathways potentially involved in TYR effects on C. elegans longevity.

Methods

Nematodes were treated with 0 or 250 μM TYR, total RNA was isolated at the adult stage, and derived cDNA probes were hybridized to Affymetrix C. elegans expression arrays. Microarray data analysis was performed, and relative mRNA expression of selected genes was validated using qPCR.

Results

Microarray analysis identified 208 differentially expressed genes (206 over-expressed and two under-expressed) when comparing TYR-treated nematodes with vehicle-treated controls. Many of these genes are linked to processes such as regulation of growth, transcription, reproduction, lipid metabolism and body morphogenesis. Moreover, we detected an interesting overlap between the expression pattern elicited by TYR and those induced by other dietary polyphenols known to extend lifespan in C. elegans, such as quercetin and tannic acid.

Conclusions

Our results suggest that important cellular mechanisms directly related to longevity are influenced by TYR treatment in C. elegans, supporting our previous notion that this phenol might act on conserved genetic pathways to increase lifespan in a whole organism.

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Acknowledgments

The authors thank the Caenorhabditis Genetics Center for providing the strains used in this study. This work was supported by the research grant R1/13/2010/02 from the Plan de Apoyo a la Investigación, Desarrollo Tecnológico e Innovación of the University of Jaén (Spain). The microarray analysis was performed in the Genomics Unit of the Research Support Services of the University of Córdoba, Spain. Also, the authors thank Mercedes Cousinou Rodríguez and Laura Redondo Sánchez for their technical assistance.

Conflict of interest

Ana Cañuelo, Francisco J. Esteban and Juan Peragón declare that they have no conflict of interest.

Ethical standard

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, 23071, Jaén, Spain

    Ana Cañuelo & Juan Peragón

  2. Cellular Biology Section, Department of Experimental Biology, University of Jaén, Jaén, Spain

    Francisco J. Esteban

Authors
  1. Ana Cañuelo
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  2. Francisco J. Esteban
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Correspondence to Ana Cañuelo.

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Cañuelo, A., Esteban, F.J. & Peragón, J. Gene expression profiling to investigate tyrosol-induced lifespan extension in Caenorhabditis elegans . Eur J Nutr 55, 639–650 (2016). https://doi.org/10.1007/s00394-015-0884-3

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