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Skeletal muscles respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days

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Abstract

Purpose

Although amino acids (AA) are required for growth, little is known about the effect of a deficient AA supply on the composition and the contractile and metabolic properties of skeletal muscles.

Methods

Protein metabolism, oxidative catabolism, glutathione system, and fiber-type composition of the longissimus (LM), rhomboideus (RM), and semitendinous (SM) muscles were compared between 42-day-old piglets pair-fed for 10 days either with a diet with a 28 % deficient supply of total sulfur AA (TSAA−) or with a diet with a sufficient supply of total sulfur AA (TSAA+).

Results

The relative weight, protein mass, and protein synthesis of LM were 10–32 % lower in TSAA− pigs compared with TSAA+ pigs, while RM and SM were not affected by the TSAA supply. The TSAA supply affected the AA composition of muscles. Concentrations of Met and branched-chain AA were, respectively, 7 and 3 % lower in TSAA− pigs compared with TSAA+ pigs. The His concentration was 30 % higher in LM and SM in TSAA− pigs compared with TSAA+ pigs and unaffected in RM. The activity of citrate synthase was 14 % higher in all three muscles of TSAA− pigs. In these pigs, the β-hydroxy-acyl-CoA dehydrogenase activity was 20 % higher in RM compared with TSAA+ pigs while that of lactate dehydrogenase was 21 % lower in LM. Total and reduced glutathione concentrations were more than 70 % greater in RM than in LM or SM, and these concentrations were approximately 10 % lower in TSAA− pigs than in TSAA+ pigs.

Conclusions

Results of this study indicate that a TSAA deficiency affects muscle properties in a muscle-dependent manner increasing the oxidative capacity of RM and reducing growth and glycolytic metabolism of LM.

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Acknowledgments

This research was supported by the Rhodimet Research Grant 2010 from Adisseo S.A.S. (France). The authors gratefully acknowledge Nathalie Bonhomme, Carlos Cobo-Ortega, Patrick Ecolan, Philippe Ganier, Yolande Jaguelin, Nadine Mézière, Anne Pasquier and Jean Noël Thibault for their excellent assistance with sampling and laboratory analyses, Alain Chauvin, Regis Janvier, Francis Le Gouevec and Vincent Piedvache, for surgical procedures and animal care, Maurice Alix, Georges Guillemois, Jérôme Liger and Jean-François Rouaud for diet preparation and slaughter procedures (INRA, Saint-Gilles, France). J.A.C-A., L.L., S.T., Y.M., and N.L.F., J.V.M. designed and conducted the research, wrote the paper and had primary responsibility for the final content; J.A.C-A. analyzed the data. All authors read and approved the final manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. INRA, UMR1348 PEGASE, 35590, Saint-Gilles, France

    José Alberto Conde-Aguilera, Louis Lefaucheur, Nathalie Le Floc’h & Jaap van Milgen

  2. Agrocampus Ouest, UMR1348 PEGASE, 35000, Rennes, France

    José Alberto Conde-Aguilera, Louis Lefaucheur, Nathalie Le Floc’h & Jaap van Milgen

  3. INRA, UR83 Recherches Avicoles, 37380, Nouzilly, France

    Sophie Tesseraud

  4. Adisseo France SAS, 92160, Antony, France

    Yves Mercier

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  1. José Alberto Conde-Aguilera
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  2. Louis Lefaucheur
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  3. Sophie Tesseraud
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  4. Yves Mercier
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  5. Nathalie Le Floc’h
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Correspondence to Jaap van Milgen.

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Conde-Aguilera, J.A., Lefaucheur, L., Tesseraud, S. et al. Skeletal muscles respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days. Eur J Nutr 55, 117–126 (2016). https://doi.org/10.1007/s00394-014-0830-9

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  • DOI: https://doi.org/10.1007/s00394-014-0830-9

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