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Increased pyruvate dehydrogenase kinase expression in cultured myotubes from obese and diabetic individuals

European Journal of Nutrition volume 54, pages 1033–1043 (2015)Cite this article

Abstract

Purpose

To investigate the mechanisms of impairments in oxidative metabolism in obese and diabetic (T2DM) skeletal muscle, this study analysed the adaptive expression of genes involved in fatty acid (FA) oxidation and mitochondrial biogenesis in primary myotubes treated with elevated FAs.

Methods

Muscle samples from obese or obese T2DM donors were stored or processed into human primary skeletal muscle myotubes, which were treated for 6 h with a saturated (palmitic acid) or a monounsaturated (oleic acid) FA with or without a polyunsaturated FA (eicosapentaenoic acid: EPA). Real-time PCR analysis was used to determine mRNA expression.

Results

Basal pyruvate dehydrogenase kinase 4 (PDK4) mRNA expression in whole muscle samples from obese and T2DM subjects was increased compared to lean (P < 0.05; n = 13–20/group). In obese- and T2DM-derived myotubes, oleic acid treatment alone and in combination with EPA increased PDK4 mRNA expression compared to control (P < 0.05; n = 7/group), whereas palmitic acid alone and in combination with EPA only increased PDK4 mRNA in T2DM-derived myotubes compared to control (P < 0.05; n = 7/group). EPA alone did not alter mRNA expression of PDK4.

Conclusions

These findings show that FAs induce the expression of PDK4 mRNA, which was increased in myotubes cultured from obese and T2DM donors. This persistent difference in PDK4 expression, present after culturing, suggests a fundamental alteration in the FA-mediated gene expression. This may in turn translate to differences in the regulation of oxidative substrate flux to impact on insulin sensitivity.

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Acknowledgments

LMC was supported by a scholarship (PB 10M 5472) from the National Heart Foundation of Australia. This work was supported by the Australian Government’s Collaborative Research Networks (CRN) programme (AJM) and the National Health and Medical Research Council (DCS). We gratefully acknowledge the invaluable contributions of the surgeons, Dr. Simon Woods FRACS, Monash University Academic Surgery Unit, Cabrini Hospital and Dr Mark Lawrence, Network Director, Obstetrics and Gynaecology, Bayside Health.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Authors and Affiliations

  1. Centre for Chronic Disease Prevention and Management, College of Health and Biomedicine, Victoria University, P.O. Box 14428, Melbourne, VIC, 8001, Australia

    A. J. McAinch & L. M. Cornall

  2. School of Exercise, Australian Catholic University, Melbourne, VIC, 3065, Australia

    R. Watts

  3. Department of Physiology, The University of Melbourne, Melbourne, VIC, 3010, Australia

    D. H. Hryciw

  4. Centre for Obesity Research and Education (CORE), The Alfred Hospital, Monash University, Melbourne, VIC, 3004, Australia

    P. E. O’Brien

  5. Liggins Institute, University of Auckland, Auckland, 1142, New Zealand

    D. Cameron-Smith

Authors
  1. A. J. McAinch
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  2. L. M. Cornall
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  3. R. Watts
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  4. D. H. Hryciw
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  5. P. E. O’Brien
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Correspondence to A. J. McAinch.

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McAinch, A.J., Cornall, L.M., Watts, R. et al. Increased pyruvate dehydrogenase kinase expression in cultured myotubes from obese and diabetic individuals. Eur J Nutr 54, 1033–1043 (2015). https://doi.org/10.1007/s00394-014-0780-2

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  • DOI: https://doi.org/10.1007/s00394-014-0780-2

Keywords

  • Fatty acids
  • mRNA
  • Metabolism
  • Skeletal muscle
  • PDK4