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Pulverized konjac glucomannan ameliorates oxazolone-induced colitis in mice

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Abstract

Purpose

Pulverized konjac glucomannan (PKGM) is a natural biologically active compound extracted from konjac, a Japanese traditional food. In the present study, we investigated the role of PKGM in intestinal immunity in a mouse model of oxazolone (OXA)-induced colitis.

Methods

C57BL/6(B6) mice were fed PKGM or control food from 2 weeks before the induction of OXA colitis. Body weight change, colon length, and histological change in the colon were examined. The mononuclear cells were purified from colon and stimulated with PMA/ionomycin. The levels of TNF-α, interferon (IFN)-γ, interleukin (IL)-4, and IL-13 from the supernatant were measured by ELISA.

Results

Oral administration of PKGM prevented the body weight loss and shortening of colon length associated with OXA-induced colitis. Histological analysis revealed that the colonic inflammation was improved by the administration of PKGM. The levels of IL-4 and IL-13, the critical inflammatory cytokines in OXA colitis, derived from mononuclear cells from the lamina propria of the colon were significantly suppressed by PKGM administration. PKGM-fed mice showed a significantly lower IL-4/IFN-γ ratio in the colonic lamina propria compared with that in control-fed mice. Fluorescence-activated cell sorting analysis revealed that natural killer (NK) 1.1+ T cells in the liver were significantly decreased in PKGM-fed mice. Finally, the preventive role of PKGM in OXA-induced colitis was not observed in invariant natural killer T cell-deficient mice.

Conclusions

PKGM ameliorated OXA-induced colitis in mice. This effect is associated with a decreased population of NK1.1+ T cells and induction of Th1-polarized immune responses.

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Abbreviations

AD:

Atopic dermatitis

iNKT:

Invariant natural killer T

KGM:

Konjac glucomannan

LPMCs:

Lamina propria mononuclear cells

OXA:

Oxazolone

PKGM:

Pulverized konjac glucomannan

UC:

Ulcerative colitis

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Acknowledgments

We thank the Shimizu Chemical Co. and Nishikawa Rubber Co. for the donation of the PA (PROPOL®) and PKGM, respectively. This work was carried out at the Analysis Center of Life Science, Natural Science Center for Basic Research and Development, Hiroshima University.

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Authors and Affiliations

  1. Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Toshiko Onitake, Shintaro Sagami, Ryohei Hayashi, Kenta Nagai & Kazuaki Chayama

  2. Department of Endoscopy, Hiroshima University Hospital, Hiroshima, Japan

    Yoshitaka Ueno & Shinji Tanaka

  3. Department of Dermatology, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Michihiro Hide

Authors
  1. Toshiko Onitake
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  2. Yoshitaka Ueno
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  5. Ryohei Hayashi
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  8. Kazuaki Chayama
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Correspondence to Yoshitaka Ueno.

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Onitake, T., Ueno, Y., Tanaka, S. et al. Pulverized konjac glucomannan ameliorates oxazolone-induced colitis in mice. Eur J Nutr 54, 959–969 (2015). https://doi.org/10.1007/s00394-014-0772-2

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  • DOI: https://doi.org/10.1007/s00394-014-0772-2

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