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Whey peptide ingestion suppresses body fat accumulation in senescence-accelerated mouse prone 6 (SAMP6)

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Abstract

Purpose

Body weight in young growing and young adult animals was reduced by a high dietary density of whey protein concentrate; however, it is unclear whether dietary proteins similarly affect body weight in aging animals. Here, we examined whether whey protein or whey peptide ingestion suppressed body fat accumulation and affected protein expression and phosphorylation in skeletal muscle in aging mice.

Methods

Twenty-six male senescence-accelerated mouse prone 6 (SAMP6) mice were assigned randomly to three dietary treatment groups: 18.7 % casein control (CON), 18.7 % whey protein (WPR), and 18.7 % whey peptide (WPE). After 28 weeks of treatment, skeletal tissues were dissected and weighed for analysis. Western blotting was performed to examine the expression of AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), and adipose triglyceride lipase (ATGL) in quadriceps muscles.

Results

Body (CON: 47.6 ± 2.2 g, WPR: 48.2 ± 2.7 g, WPE: 38.3 ± 2.0 g) and relative white adipose tissue (CON: 38.5 ± 3.5 mg/g, WPR: 43.8 ± 4.0 mg/g, WPE: 21.1 ± 4.4 mg/g) weights were lower in the WPE group compared with the other two groups (p < 0.05), and no significant differences were observed between the CON and WPR groups. The relative weights of tibialis anterior muscle (CON: 1.04 ± 0.04 mg/g, WPR: 0.97 ± 0.03 mg/g, 1.23 ± 0.05 mg/g) and gastrocnemius muscle (CON: 3.02 ± 0.12 mg/g, WPR: 2.92 ± 0.15 mg/g, WPE: 3.65 ± 0.18 mg/g) were higher in the WPE group compared with the other groups (p < 0.05). The phosphorylation of AMPK (WPR: 1.03 ± 0.11, WPE: 1.36 ± 0.12; fold change from control) and ACC (WPR: 1.08 ± 0.07, WPE: 1.18 ± 0.05; fold change from control) in WPE was higher than in CON (p < 0.05). There were no significant differences in the expression levels of ATGL among the three groups.

Conclusions

These data suggest that a normal (or moderate excess) dietary density of whey peptide attenuates body fat accumulation via upregulation of fatty acid oxidation in skeletal muscle in aging mice.

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Conflict of interest

The authors have read the journal’s policy and declare the following conflicts: SM is an employee of Megmilk Snow Brand Co., Ltd, Japan and HN received a research fund from Megmilk Snow Brand Co., Ltd. The sponsor had no control over the study design, decision to publish, or preparation of the manuscript. There are no patents, products in development, or marketed products to declare.

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Authors and Affiliations

  1. Graduate School of Health and Sports Science, Juntendo University, 1-1 Hiragagakuendai, Inzai, Chiba, 2701695, Japan

    Noriko Ichinoseki-Sekine & Hisashi Naito

  2. School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 1138421, Japan

    Ryo Kakigi

  3. Megmilk Snow Brand Co., Ltd., 13 Honshiocho, Shinjuku-ku, Tokyo, 1608575, Japan

    Susumu Miura

Authors
  1. Noriko Ichinoseki-Sekine
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  2. Ryo Kakigi
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  3. Susumu Miura
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  4. Hisashi Naito
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Corresponding author

Correspondence to Hisashi Naito.

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394_2014_736_MOESM1_ESM.tif

Representative gel of the four isoforms (I, IIa, IIx and IIb) of skeletal muscle myosin heavy chain (MHC) in the control (CON), whey protein (WPR), and whey peptide (WPE) dietary groups. (TIFF 942 kb)

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Ichinoseki-Sekine, N., Kakigi, R., Miura, S. et al. Whey peptide ingestion suppresses body fat accumulation in senescence-accelerated mouse prone 6 (SAMP6). Eur J Nutr 54, 551–556 (2015). https://doi.org/10.1007/s00394-014-0736-6

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  • DOI: https://doi.org/10.1007/s00394-014-0736-6

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