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The relation of diet with PAF and its metabolic enzymes in healthy volunteers

  • Original Contribution
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European Journal of Nutrition Aims and scope Submit manuscript

Abstract

Purpose

Platelet-activating factor (PAF), a potent inflammatory mediator, is implicated in atherosclerosis. Its key biosynthetic enzymes are lyso-PAF acetyltransferases (lyso-PAF-AT), responsible for PAF synthesis through the remodeling route and a specific CDP-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-CPT), responsible for its de novo biosynthesis. PAF acetylhydrolase (PAF-AH) and its extracellular isoform lipoprotein-associated phospholipase A2 catabolize PAF. The impact of diet on PAF metabolism is ill-defined. The aim was to investigate associations between PAF, its enzymes and dietary factors.

Methods

One-hundred and six (n = 106) healthy volunteers were recruited. Food-frequency questionnaires, dietary recalls, lifestyle and biochemical variables were collected. Food groups, macronutrient intake, a priori (MedDietScore) and a posteriori defined food patterns with PCA analysis, dietary antioxidant capacity (DAC), glycemic index (GI) and glycemic load were assessed.

Results

PAF was inversely correlated with antioxidant-rich foods (herbal drinks and coffee), the DAC as well as a dietary pattern characterized by legumes, vegetables, poultry and fish (all Ps < 0.05). PAF was positively correlated to % fat intake. Lyso-PAF-AT was also negatively associated with healthy patterns (fruits, nuts and herbal drinks, and a pattern rich in olive oil and whole-wheat products), as well as the DAC and % monounsaturated fatty acids. PAF-CPT was negatively associated with GI and coffee intake and positively with dietary cholesterol. PAF-AH was negatively associated with coffee and positively associated with alcohol consumption (all Ps < 0.05).

Conclusions

In conclusion, the DAC and healthy dietary patterns were inversely associated with PAF or its biosynthetic enzymes, suggesting potential new mechanisms of the diet–disease associations.

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Abbreviations

GL:

Glycemic load

GI:

Glycemic index

PAF:

Platelet-activating factor

Lyso-PAF-AT:

Acetyl-CoA: lyso-PAF acetyltransferase

PAF-CPT:

DTT-insensitive CDP-choline1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase

PAF-AH:

PAF acetylhydrolase

Lp-PLA2 :

Lipoprotein-associated phospholipase A2

DAC:

Dietary antioxidant capacity

PCA:

Principal component analysis

TRAP:

Total radical-trapping antioxidant parameters

FRAP:

Ferric-reducing antioxidant power

TEAC:

Trolox equivalent antioxidant capacity

EI:

Energy intake

BMR:

Basal metabolic rate

KMO:

Kaiser–Mayer–Oklin

MUFA:

Monounsaturated fatty acids

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Acknowledgments

The authors would like to thank Dr. M. Xanthopoulou and Mrs. S. Letsiou for their assistance in blood handling, Mrs. O. Kounari for her support in data collection and Mrs. M. Christea for her support in blood collection. The study was funded by the Hellenic Atherosclerosis Society. The funding source was not involved in study design, the collection, analysis and interpretation of data, in the writing of the report, nor in the decision to submit the article for publication.

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Authors and Affiliations

  1. Department of Nutrition-Dietetics, Harokopio University, 70 El. Venizelou Street, 17671, Athens, Greece

    P. Detopoulou, E. Fragopoulou, T. Nomikos, M. Yannakoulia, D. B. Panagiotakos & S. Antonopoulou

  2. Department of Chemistry, National and Kapodistrian University of Athens, Panepistimioupolis, 15771, Athens, Greece

    G. Stamatakis

Authors
  1. P. Detopoulou
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  2. E. Fragopoulou
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  3. T. Nomikos
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  4. M. Yannakoulia
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  6. D. B. Panagiotakos
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  7. S. Antonopoulou
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Correspondence to S. Antonopoulou.

Additional information

P. Detopoulou received Fellowship from the State Scholarship Foundation (IKY) and the Hellenic Atherosclerosis Society.

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Detopoulou, P., Fragopoulou, E., Nomikos, T. et al. The relation of diet with PAF and its metabolic enzymes in healthy volunteers. Eur J Nutr 54, 25–34 (2015). https://doi.org/10.1007/s00394-014-0682-3

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  • DOI: https://doi.org/10.1007/s00394-014-0682-3

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