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Incorporation of conjugated linoleic acid isomers into porcine erythrocytes

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Abstract

Purpose

The aim of the current study was to determine the incorporation of cis (c) 9, trans (t) 11-conjugated linoleic acid (CLA) and t10, c12-CLA into porcine erythrocytes—both isomers were supplemented in equal proportions.

Methods

The study group consisted of 16 piglets randomly assigned into experimental and control group. For the period of 5 weeks, the piglets from the experimental group were receiving a 1.2 % CLA supplement while the controls were supplemented with the same amount of sunflower oil. For the remaining 7 weeks, the piglets were fed without a supplement. Blood samples to evaluate incorporation of CLA into erythrocyte membranes were taken from all animals on weekly basis.

Results

Compared to t10, c12-CLA isomer, proportion of c9, t11-CLA isomer in the membrane of erythrocytes was higher for the whole time of the study period. After 4 weeks of feeding, it approaches the plateau. The peak value for both isomers was measured at the end of week 5, with a value of 3.24 g c9, t11-CLA/100 g of fatty acids and a 1.09 g t10, c12-CLA/100 g of fatty acids (p < 0.0001). After cessation of supplementation, the proportion of both isomers gradually decreased to be almost completely washed out—in 7 weeks.

Conclusions

During supplementation with equivalent amounts of CLA isomers, their proportion in membranes of porcine erythrocytes increases with time, with higher proportion of c9, t11-CLA. CLA isomers probably differently incorporate into different cell membranes at different species which could explain its various biological functions.

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Acknowledgment

This study was supported by Slovenian government P4-0092 research grant. The ethicalness of the study protocol was approved by the Slovenian Veterinary Administration (4.4.-23/05). We are grateful to Dr. Gaj Vidmar from the Institute of Rehabilitation, Republic of Slovenia, for help with statistical analyses.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Authors and Affiliations

  1. University Medical Centre Ljubljana, Ljubljana, Slovenia

    Tomaž Malovrh & Enver Melkić

  2. Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    Drago Kompan & Alenka Levart

  3. Institute of Oncology, Ljubljana, Slovenia

    Lidija Kompan

Authors
  1. Tomaž Malovrh
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  2. Enver Melkić
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  3. Drago Kompan
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  4. Alenka Levart
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  5. Lidija Kompan
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Correspondence to Lidija Kompan.

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Malovrh, T., Melkić, E., Kompan, D. et al. Incorporation of conjugated linoleic acid isomers into porcine erythrocytes. Eur J Nutr 53, 989–993 (2014). https://doi.org/10.1007/s00394-013-0618-3

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  • DOI: https://doi.org/10.1007/s00394-013-0618-3

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