Pomegranate and its derivatives can improve bone health through decreased inflammation and oxidative stress in an animal model of postmenopausal osteoporosis
- 836 Downloads
Recently, nutritional and pharmaceutical benefits of pomegranate (PG) have raised a growing scientific interest. Since PG is endowed with anti-inflammatory and antioxidant activities, we hypothesized that it may have beneficial effects on osteoporosis.
We used ovariectomized (OVX) mice as a well-described model of postmenopausal osteoporosis to study the influence of PG consumption on bone health. Mice were divided into five groups as following: two control groups sham-operated and ovariectomized (OVX CT) mice fed a standard diet, versus three treated groups OVX mice given a modified diet from the AIN-93G diet, containing 5.7 % of PG lyophilized mashed totum (OVX PGt), or 9.6 % of PG fresh juice (OVX PGj) or 2.9 % of PG lyophilized mashed peel (OVX PGp).
As expected, ovariectomy was associated with a decreased femoral bone mineral density (BMD) and impaired bone micro-architecture parameters. Consumption of PGj, PGp, or PGt induced bone-sparing effects in those OVX mice, both on femoral BMD and bone micro-architecture parameters. In addition, PG (whatever the part) up-regulated osteoblast activity and decreased the expression of osteoclast markers, when compared to what was observed in OVX CT animals. Consistent with the data related to bone parameters, PG consumption elicited a lower expression of pro-inflammatory makers and of enzymes involved in ROS generation, whereas the expression of anti-inflammatory markers and anti-oxidant actors was enhanced.
These results indicate that all PG parts are effective in preventing the development of bone loss induced by ovariectomy in mice. Such an effect could be partially explained by an improved inflammatory and oxidative status.
KeywordsPomegranate Nutritional prevention Osteoporosis Animal model Inflammation Oxidative stress
Greentech (GREENTECH SA, Saint-Beauzire, France) is kindly acknowledged for providing financial support for this study. The authors are as well grateful to (1) Paul Pilet for his assistance in collecting data of bone micro-architecture from micro-CT, (2) the people from the “Animal lab: Installation Experimentale de Nutrition” who provided every day cares to mice. This study was supported by INRA, UMR 1019, UNH, Clermont-Ferrand, France.
Conflict of interest
The authors have no conflict of interest to declare.
- 1.Kim ND, Mehta R, Yu W, Neeman I, Livney T, Amichay A, Poirier D, Nicholls P, Kirby A, Jiang W, Mansel R, Ramachandran C, Rabi T, Kaplan B, Lansky E (2002) Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast Cancer Res Treat 71(3):203–217CrossRefGoogle Scholar
- 10.Balbir-Gurman A, Fuhrman B, Braun-Moscovici Y, Markovits D, Aviram M (2011) Consumption of pomegranate decreases serum oxidative stress and reduces disease activity in patients with active rheumatoid arthritis: a pilot study. Isr Med Assoc J 13(8):474–479Google Scholar
- 13.Schulman RC, Weiss AJ, Mechanick JI (2011) Nutrition, bone, and aging: an integrative physiology approach. Current osteoporosis reports 9 (4):184–195. doi: 10.1007/s11914-011-0079-7
- 16.Park EK, Kim MS, Lee SH, Kim KH, Park JY, Kim TH, Lee IS, Woo JT, Jung JC, Shin HI, Choi JY, Kim SY (2004) Furosin, an ellagitannin, suppresses RANKL-induced osteoclast differentiation and function through inhibition of MAP kinase activation and actin ring formation. Biochem Biophys Res Commun 325(4):1472–1480. doi: 10.1016/j.bbrc.2004.10.197 CrossRefGoogle Scholar
- 19.Rosillo MA, Sanchez-Hidalgo M, Cardeno A, Aparicio-Soto M, Sanchez-Fidalgo S, Villegas I, de la Lastra CA (2012) Dietary supplementation of an ellagic acid-enriched pomegranate extract attenuates chronic colonic inflammation in rats. Pharmacol Res 66(3):235–242. doi: 10.1016/j.phrs.2012.05.006 CrossRefGoogle Scholar
- 29.Gautam AK, Bhargavan B, Tyagi AM, Srivastava K, Yadav DK, Kumar M, Singh A, Mishra JS, Singh AB, Sanyal S, Maurya R, Manickavasagam L, Singh SP, Wahajuddin W, Jain GK, Chattopadhyay N, Singh D (2011) Differential effects of formononetin and cladrin on osteoblast function, peak bone mass achievement and bioavailability in rats. J Nutr Biochem 22(4):318–327. doi: 10.1016/j.jnutbio.2010.02.010 CrossRefGoogle Scholar
- 30.Monsefi M, Parvin F, Talaei-Khozani T (2011) Effects of pomegranate extracts on cartilage, bone and mesenchymal cells of mouse fetuses. BrJ Nutr 1–8. doi: 10.1017/S0007114511003394