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Plasma kinetics of chylomicron-like emulsion and lipid transfers to high-density lipoprotein (HDL) in lacto-ovo vegetarian and in omnivorous subjects

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Abstract

Purpose

Previously, it was showed that vegan diet improves the metabolism of triglyceride-rich lipoproteins by increasing the plasma clearance of atherogenic remnants. The aim of the current study was to investigate this metabolism in lacto-ovo vegetarians whose diet is less strict, allowing the ingestion of eggs and milk. Transfer of lipids to HDL, an important step in HDL metabolism, was tested in vitro.

Methods

Eighteen lacto-ovo vegetarians and 29 omnivorous subjects, all eutrophic and normolipidemic, were intravenously injected with triglyceride-rich emulsions labeled with 14C-cholesterol oleate and 3H-triolein. Fractional clearance rates (FCR, in min−1) were calculated from samples collected during 60 min. Lipid transfer to HDL was assayed by incubating plasma samples with a donor nanoemulsion labeled with radioactive lipids.

Results

LDL cholesterol was lower in vegetarians than in omnivores (2.1 ± 0.8 and 2.7 ± 0.7 mmol/L, respectively, p < 0.05), but HDL cholesterol and triglycerides were equal. Cholesteryl ester FCR was greater in vegetarians than in omnivores (0.016 ± 0.012, 0.003 ± 0.003, p < 0.01), whereas triglyceride FCR was equal. Cholesteryl ester transfer to HDL was lower in vegetarians than in omnivores (2.7 ± 0.6, 3.5 ± 1.5 %, p < 0.05), but free cholesterol, triglyceride and phospholipid transfers and HDL size were equal.

Conclusion

Similarly to vegans, lacto-ovo vegetarian diet increases remnant removal, as indicated by cholesteryl oleate FCR, which may favor atherosclerosis prevention, and has the ability to change lipid transfer to HDL.

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References

  1. Craig WJ, Mangels AR (2009) Position of the American Dietetic Association: vegetarian diets. J Am Dietetic Assoc 109(7):1266–1282

    Google Scholar 

  2. Vinagre JC, Vinagre CG, Pozzi FS, Slywitch E, Maranhão RC (2013) Metabolism of triglyceride-rich lipoproteins and transfer of lipids to high-density lipoproteins (HDL) in vegan and omnivore subject. Nutr Metab Cardiovasc 23:61–67

    Article  CAS  Google Scholar 

  3. Karpe F, Steiner G, Uffelman K, Olivecrona T, Hamsten A (1994) Postprandial lipoproteins and progression of coronary atherosclerosis. Atherosclerosis 106:83–97

    Article  CAS  Google Scholar 

  4. Martins MC, Pileggi F, Maranhão RC (1995) Clearance of a chylomicron-like emulsion from plasma is delayed in patients with coronary artery disease. Braz J Med Biol Res 28:427–431

    CAS  Google Scholar 

  5. Sposito AC, Lemos PA, Santos RD, Hueb W, Vinagre CG, Maranhão RC (2004) Impaired intravascular triglyceride lipolysis constitutes a marker of clinical outcome in patients with stable angina undergoing secondary prevention treatment: a long-term follow-up study. J Am Coll Cardiol 43:2225–2232

    Article  CAS  Google Scholar 

  6. Sposito AC, Ventura LI, Vinagre CG, Lemos PA, Santos RD, Maranhão RC (2004) Delayed intravascular catabolism of chylomicron-like emulsions is an independent predictor of coronary artery disease. Atherosclerosis 176:397–403

    Article  CAS  Google Scholar 

  7. César TB, Oliveira MR, Mesquita CH, Maranhão RC (2006) High cholesterol intake modifies chylomicron metabolism in normolipidemic young men. J Nutr 136:971–976

    Google Scholar 

  8. Maranhão RC, Feres MC, Martins MT, Mesquita CH, Toffoletto O, Vinagre CG et al (1996) Plasma kinetics of a chylomicron-like emulsion in patients with coronary artery disease. Atherosclerosis 126:15–21

    Article  Google Scholar 

  9. Berr F, Kern F (1984) Plasma clearance of chylomicrons labeled with retinyl palmitate in healthy human subjects. J Lipid Res 38:301–314

    Google Scholar 

  10. Demacker PN, Reijnen IG, Katan MB, Stuyt PM, Stalenhoef AF (1991) Increased removal of remnants of triglyceride-rich lipoproteins on a diet rich in polyunsaturated fatty acids. Eur J Clin Invest 21:197–203

    Article  CAS  Google Scholar 

  11. Redgrave TG (2004) Chylomicron metabolism. Biochem Soc Trans 32(1):79–82

    Google Scholar 

  12. Asztalos BF, Schaefer EJ (2003) High-density lipoprotein subpopulations in pathologic conditions. Am J Cardiol 91(suppl):12–17

    Article  CAS  Google Scholar 

  13. Wang M, Briggs MR (2004) HDL: the metabolism, function, and therapeutic importance. Chem Rev 104:119–137

    Article  CAS  Google Scholar 

  14. Schaefer EJ, Zech LA, Jenkins LL et al (1982) Human apolipoprotein A-I and A-II metabolism. J Lipid Res 23:850–862

    CAS  Google Scholar 

  15. vonEckardstein A, Nofer JR, Assmann G (2001) High density lipoproteins and arteriosclerosis: role of cholesterol efflux and reverse cholesterol transport. Arterioscler Thromb Vasc Biol 21:13–27

    Article  CAS  Google Scholar 

  16. Lewis GF, Rader DJ (2005) New insights into the regulation of HDL metabolism and reverse cholesterol transport. Circ Res 96:1221

    Article  CAS  Google Scholar 

  17. Mahley RW, Huang Y, Weisgraber KH (2006) Putting cholesterol in its place: apo E and reverse cholesterol transport. J Clin Invest 116:1126–1129

    Article  CAS  Google Scholar 

  18. Kontush A, Chapman MJ (2006) Antiatherogenic small, dense HDL—guardian angel of the arterial wall? Nat Clin Pract Cardiovasc Med 3:144–153

    Article  CAS  Google Scholar 

  19. Lo Prete AC, Dina CH, Azevedo CH, Puk CG, Hueb WA, Maranhão RC (2009) In vitro simultaneous transfer of lipids to HDL in coronary artery disease and in Statin treatment. Lipids 44:917–924

    Article  CAS  Google Scholar 

  20. Lima ES, Maranhão RC (2004) Rapid, simple laser-light-scattering method for HDL particle sizing in whole plasma. Clin Chem 50:1086–1088

    Article  CAS  Google Scholar 

  21. Ehnholm C, Kuusi T (1986) Preparation, characterization, and measurement of hepatic lipase. Methods Enzymol 129:716–738

    Article  CAS  Google Scholar 

  22. Lambert MS, Avela MA, Botham KM, Mayes PA (2000) The type of dietary fat alters the hepatic uptake and biliary excretion of cholesterol from chylomicron remnants. Br J Nutr 83:431–438

    CAS  Google Scholar 

  23. De Biase SG, Fernandes SF, Gianini RJ, Duarte JL (2007) Vegetarian diet and cholesterol and triglyceride levels. Arq Bras Cardiol 88(1):35–39

    Google Scholar 

  24. Hung CJ, Huang PC, Li YH, Lu SC, Ho LT, Chou HF (2006) Taiwanese vegetarians have higher insulin sensitivity than omnivores. Br J Nutr 95:129–135

    Article  CAS  Google Scholar 

  25. Griffin MD, Sanders TA, Davies IG, Morgan LM, Millward DJ, Lewis F et al (2006) Effects of altering the ratio of dietary n-6 to n-3 fatty acids on insulin sensitivity, lipoprotein size, and post- prandial lipemia in men and postmenopausal women aged 45-70y: the OPTILIP Study. Am J Clin Nutr 84:1290–1298

    CAS  Google Scholar 

  26. Zampelas A, Peel AS, Gould BJ, Wright J, Williams CM (1994) Polyunsaturated fatty acids of the n-6 and n-3 series: effects on postprandial lipid and apolipoprotein levels in healthy menEur. J Clin Nutr 48:842–848

    CAS  Google Scholar 

  27. Grieve DJ, Avella MA, Botham KM, Elliott J (2000) Evidence that chylomicron remnants potentiate phenylephrine-induced contractions of rat aorta by an endothelium-dependent mechanism. Atherosclerosis 151:471–480

    Article  CAS  Google Scholar 

  28. Napolitano M, Rivabene R, Avella M, Botham KM, Bravo E (2001) The internal redox balance of the cells influences the metabolism of lipids of dietary origin by J774 macrophages: implications for foam cell formation. J Vasc Res 38:350–360

    Article  CAS  Google Scholar 

  29. Kawasaki S, Taniguchi T, Fujioka Y, Takahashi A, Takahashi T, Domoto K et al (2000) Chylomicron remnant induces apoptosis in vascular endothelial cells. Ann N Y Acad Sci 902:336–341

    Article  CAS  Google Scholar 

  30. Doi H, Kugiyama K, Oka H, Sugiyama S, Ogata N, Koide SI et al (2000) Remnant lipoproteins induce proatherothrombogenic molecules in endothelial cells through a redox-sensitive mechanism. Circulation 102:670–676

    Google Scholar 

  31. Kawakami A, Tanaka A, Nakano T, Saniabadi A, Numano F (2001) Stimulation of arterial smooth muscle cell proliferation by remnant lipoprotein particles isolated by immuno-affinity chromatography with anti-apo A-I and anti-apo B-100. Horm Metab Res 33:67–72

    Article  CAS  Google Scholar 

  32. Saniabadi AR, Umemura K, Shimoyama M, Adachi M, Nakano M, Nakashima M (1997) Aggregation of human blood platelets by remnant like lipoprotein particles of plasma chylomicrons and very low density lipoproteins. Thromb Haemost 77:996–1001

    CAS  Google Scholar 

  33. Groener JEM, Van Ramshorst EM, Katan MB, Mensink RP, Van Tol A (1991) Diet induced alteration in the activity of plasma lipid transfer protein in normolipidemic human subjects. Atherosclerosis 87:221–226

    Article  CAS  Google Scholar 

  34. Schwab US, Maliranta HM, Sarkkinen ES, Savolainen MJ, Kesäniemi YA, Uusitupa MI (1996) Different effects of palmitic and stearic acid-enriched diets on serum lipids and lipoproteins and plasma cholesteryl ester transfer protein activity in healthy young women. Metabolism 45:143–149

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Heart Institute (InCor) of the Medical School Hospital, University of São Paulo, São Paulo, Brazil

    Juliana C. Vinagre, Carmen C. G. Vinagre, Fernanda S. Pozzi, Cristiane Z. Zácari & Raul C. Maranhão

  2. Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil

    Raul C. Maranhão

  3. Laboratório de Metabolismo de Lípides, Instituto do Coração (InCor) do Hospital das Clínicas FMUSP, Av. Dr. Enéas de Carvalho Aguiar, 44,1° subsolo, São Paulo, SP, 05403-000, Brazil

    Raul C. Maranhão

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  1. Juliana C. Vinagre
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  2. Carmen C. G. Vinagre
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  3. Fernanda S. Pozzi
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  4. Cristiane Z. Zácari
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  5. Raul C. Maranhão
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Correspondence to Raul C. Maranhão.

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Vinagre, J.C., Vinagre, C.C.G., Pozzi, F.S. et al. Plasma kinetics of chylomicron-like emulsion and lipid transfers to high-density lipoprotein (HDL) in lacto-ovo vegetarian and in omnivorous subjects. Eur J Nutr 53, 981–987 (2014). https://doi.org/10.1007/s00394-013-0602-y

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  • DOI: https://doi.org/10.1007/s00394-013-0602-y

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