Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect
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The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model.
Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed.
All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats.
This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.
KeywordsAcute kidney injury Choline-deficient Gene expression Menhaden oil Protective effect
We thank Mariana dos Santos and Silvia Caram for technical assistance, Myrthala Duguid (Head of the Library at CEMIC University Institute), and Valeria Melia for English editing of the manuscript. This paper was partially supported by a grant from CONICET and University of Buenos Aires, Argentina.
Conflict of interest
All the authors declare no conflicts of interest in this protocol.
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