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Anti-anaemia efficacy of β-lactoglobulin hydrolysate-iron complex on iron-deficient anaemic rats

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Abstract

Purpose

The effects of orally administered β-lactoglobulin hydrolysate-iron complex (β-LGH-Fe) on haematological and biochemical parameters in anaemic rats were evaluated. Female weaning Sprague–Dawley rats were fed with iron-deficient diet to induce iron deficiency anaemia. After 6 weeks, the obtained anaemic rats were divided into five groups: iron deficiency control group (iron-deficient diet without β-LGH-Fe complex supplementation, IDC); three groups supplemented with different dosages of β-LGH-Fe complex (0.5 mg Fe/kg BW, iron-deficient diet with low β-LGH-Fe, IDLFe; 2.0 mg Fe/kg BW, iron-deficient diet with medium β-LGH-Fe, IDMF; 4.0 mg Fe/kg BW, iron-deficient diet with high β-LGH-Fe, IDHFe); and ferrous sulphate-supplemented group at a dosage of 2.0 mg Fe/kg BW.

Results

β-LGH-Fe complex could significantly improve hematocrit and haemoglobin decrease, and normalise the serum iron level, total iron-binding capacity and transferrin saturation of anaemic rats in a dose-dependent manner. Serum ferritin content and hepatic nonheme iron level were also increased. In addition, the antioxidant enzyme activities of superoxidase dismutase, catalase and glutathione peroxidase in both plasma and liver homogenate were improved. The production of malondialdehyde and pro-inflammatory cytokines (TNF-α and IL-6) decreased.

Conclusions

It suggests that β-LGH-Fe complex can ameliorate iron deficiency anaemia, which might make it a potential ingredient with anti-anaemia activity.

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Abbreviations

CTA:

Catalase

Hb:

Haemoglobin

HTC:

Haematocrit

IDA:

Iron deficiency anaemia

IL-6:

Interleukin-6

GSH-Px:

Glutathione peroxidase

MDA:

Malondialdehyde

SOD:

Superoxidase dismutase

TNF-α:

Tumour necrosis factor α

TS:

Transferrin saturation

References

  1. Andango PE, Osendarp SJ, Ayah R, West CE, Mwaniki DL, De Wolf CA, Kraaijenhagen R, Kok FJ, Verhoef H (2007) Efficacy of iron fortified whole maize flour on iron status of school children in Kenya: a randomized controlled trail. Lancet 369:1799–1806

    Article  CAS  Google Scholar 

  2. Leung AKC, Chan KW (2001) Fe deficiency anemia. Adv Pediatr 48:385–408

    CAS  Google Scholar 

  3. Beard JL (2001) Iron biology in immune function, muscle metabolism and neuronal functioning. J Nutr 131:568S–580S

    CAS  Google Scholar 

  4. Schumann K, Ettle T, Szegner B, Elsenhans B, Solomons NW (2007) On risks and benefits of iron supplementation recommendations for iron intake revisited. J Trace Elem Med Biol 21:47–68

    Article  CAS  Google Scholar 

  5. Dewey KG, Domellof M, Cohen RJ, Landa Rivera L, Hernell O, Lönnerdal B (2002) Iron supplementation affects growth and morbidity of breast-fed infants: results of a randomized trial in Sweden and Honduras. J Nutr 132:3249–3255

    CAS  Google Scholar 

  6. Hyder SM, Persson LA, Chowdhury AMR, Ekstrom EC (2002) Do side-effects reduce compliance to iron supplementation? A study of daily and weekly-dose regimens in pregnancy. J Health Popul Nutr 20:175–179

    Google Scholar 

  7. Martins EAL, Robalinho RL, Meneghini R (1995) Oxidative stress induces activation of a cytosolic protein responsible for control of iron uptake. Arch Biochem Biophys 316:128–134

    Article  CAS  Google Scholar 

  8. Ballot DE, MacPhail AP, Bothwell TH, Gillooly M, Mayet FG (1989) Fortification of curry powder with NaFe(III)EDTA in an iron deficient population. Report of a controlled iron-fortification trial. Am J Clin Nutr 49:162–169

    CAS  Google Scholar 

  9. Hurrell R (2002) How to ensure adequate iron absorption from iron-fortified food. Nutr Rev 60:7–15

    Article  Google Scholar 

  10. Chaud MV, Izumi C, Nahaal Z, Shuhama T, Bianchi Mde L, de Freitas O (2002) Iron derivatives from casein hydrolysates as a potential source in the treatment of iron deficiency. J Agric Food Chem 50:871–877

    Article  CAS  Google Scholar 

  11. Miquel E, Farre R (2007) Effects and future trends of casein phosphopeptides on zinc bioavailability. Trends Food Sci Technol 18:139–143

    Article  CAS  Google Scholar 

  12. Ani-Kibangou B, Bouhallab S, Molle D (2005) Improved absorption of caseinophosphopeptide-bound iron: role of alkaline phosphatase. J Nutr Biochem 16:398–401

    Article  CAS  Google Scholar 

  13. Nakano T, Goto T, Nakaji T, Aoki T (2007) Bioavailability of iron-fortified whey protein concentrate in iron-deficient rats. Asian Aust J Anim Sci 20:1120–1126

    CAS  Google Scholar 

  14. Argyri K, Miller DD, Glahn RP, Zhu L, Kapsokefalou M (2007) Peptides isolated from in vitro digests of milk enhance iron uptake by Caco-2 cells. J Agric Food Chem 55:10221–10225

    Article  CAS  Google Scholar 

  15. Aït-Oukhatar N, Bouhallab S, Arhan P, Maubois JL, Drosdowsky M, Bouglé D (1999) Iron tissue storage and hemoglobin levels of deficient rats repleted with iron bound to the caseinophophopeptides 1–25 of β-casein. J Agric Food Chem 47:2786–2790

    Article  Google Scholar 

  16. Aït-Oukhatar N, Bouhallab S, Bureau F, Arhan P, Maubois JL, Bouglé DL (2000) In vitro digestion of caseinophosphopeptide-iron complex. J Dairy Res 67:125–129

    Article  Google Scholar 

  17. Aït-Oukhatar N, Bouhallab S, Bureau F, Arhan P, Maubois JL, Drosdowsky MA, Bouglé DL (1997) Bioavailability of caseinophosphopeptide bound iron in the young rat. J Nutr Biochem 8:190–194

    Article  Google Scholar 

  18. Vegarud GE, Langsrud T, Svenning C (2000) Mineral-binding milk proteins and peptides; occurrence, biochemical and technological characteristics. Brit J Nutr 84:S91–S98

    Article  CAS  Google Scholar 

  19. Kim SB, Seo IS, Khan MA, Ki KS, Nam MS, Kim HS (2007) Separation of iron-binding protein from whey through enzymatic hydrolysis. Int Dairy J 17:625–631

    Article  CAS  Google Scholar 

  20. Maes W, Van Camp J, Vermeirssen V, Hemeryck M, Ketelslegers JM, Schrezenmeir J, Van Oosteveldt P, Huyghebaert A (2004) Influence of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR) on the release of endothelin-1 by endothelial cells. Regul Peptides 118:105–109

    Article  CAS  Google Scholar 

  21. Yamauchi R, Usui H, Yunden J, Takenaka Y, Tani F, Yoshikawa M (2003) Characterization of beta-lactoglobulin, a bioactive peptide derived from bovine beta-lactoglobulin, as a neurotensin agonist. Biosci Biotechnol Biochem 67:940–943

    Article  CAS  Google Scholar 

  22. Zhou J, Wang X, Ai T, Cheng X, Guo HY, Teng GX, Mao XY (2012) Preparation and characterization of β-lactoglobulin hydrolysate-iron complexes. J Dairy Sci 95:4230–4236

    Article  CAS  Google Scholar 

  23. National Research Council (1985) Guide for the care and use of laboratory animals. NIH publication no. 85. Bethesda: National Institutes of Health, pp 23

  24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  25. Zhu L, Glahn RP, Nelson D, Miller DD (2009) Comparing soluble ferric pyrophosphate to common iron salts and chelates as sources of bioavailable iron in a Caco-2 cell culture model. J Agric Food Chem 57:5014–5019

    Article  CAS  Google Scholar 

  26. da Conceicao EC, Shuhama T, Izumi C, de Freitas O (2001) Iron supplementation prevents the development of iron deficiency in rats with omeprazole-induced hypochlorhydria. Nutr Res 21:1201–1208

    Article  Google Scholar 

  27. Haro-Vicente JF, Pérez-Conesa D, Rincón F, Ros G, Martínez-Graciá C, Vidal ML (2008) Does ascorbic acid supplementation affect iron bioavailability in rats fed micronized dispersible ferric pyrophosphate fortified fruit juice? Eur J Nutr 47:470–478

    Article  CAS  Google Scholar 

  28. Thomas CE, Gaffney-Stomberg E, Sun BH, O’Brien KO, Kerstetter JE, Insogna KL (2013) Increasing dietary protein acutely augments intestinal iron transporter expression and significantly increases iron absorption in rats. FASEB J 2:2476–2483

    Article  CAS  Google Scholar 

  29. Yasuda K, Dawson HD, Wasmuth EV, Roneker CA, Chen C, Urban JF, Welch RM, Miller DD, Lei XG (2009) Supplemental dietary inulin influences expression of iron and inflammation related genes in young pigs. J Nutr 139:2018–2023

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful for the financial support of this work by the National Natural Science Foundation of China (Beijing, China; Grant No. 30972291) and Beijing Natural Science Foundation (Beijing, China; Grant No. 5102021). J. Z. and X. Y. M. worked on preparing the project design, data analysis, final revision and submission of manuscript. J. Z., T. A. and J. J. M. conducted the experiment with the animals and performed the laboratorial analysis. J. Z. and X. W. performed the statistical analysis. None of the authors had any conflict of interest related to their participation in this study. All authors have read and approved the final manuscript.

Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China

    Jie Zhou, Xue-Ying Mao, Xu Wang, Ting Ai & Jing-Jiao Ma

  2. China Astronaut Training Center, Beijing, China

    Ying-Hui Li

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  1. Jie Zhou
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  2. Xue-Ying Mao
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Correspondence to Xue-Ying Mao.

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Zhou, J., Mao, XY., Wang, X. et al. Anti-anaemia efficacy of β-lactoglobulin hydrolysate-iron complex on iron-deficient anaemic rats. Eur J Nutr 53, 877–884 (2014). https://doi.org/10.1007/s00394-013-0591-x

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  • DOI: https://doi.org/10.1007/s00394-013-0591-x

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