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Plantago maxima leaves extract inhibits adipogenic action of a high-fat diet in female Wistar rats

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Abstract

Purpose

The primary objective of this study is to investigate the content of biologically active compounds producing an antioxidant effect in Plantago maxima and their influence on main mechanisms of dietary obesity development.

Methods

Biologically active compounds in P. maxima were tested using paper chromatography. In in vivo experiment, high-fat-fed Wistar rats obtained P. maxima water extract for 3 months. Morphometric parameters, weight gain, serum adipokines, and cytokines, as well as oxidative stress biomarkers in rats’ tissues were evaluated. Gut microflora was also examined.

Results

Plantago maxima leaves used in the experiment contained significant amount of flavonoids, iridoids, phenol carboxylic acids, and tannins and ascorbic acid. Our in vivo experiment data demonstrate that P. maxima water extract prevents excessive adiposity in a diet-induced model. P. maxima consumption reduced serum leptin (twofold), macrophage chemoattractant protein-1 (sevenfold), tumor-necrosis factor-α (25 %), and interleukine-6 (26 %) levels. P. maxima water extract decreased adipose tissue oxidative stress biomarkers in rats fed a high-fat diet. In addition, increased bacterial growth in the diet-induced obesity model was reversed by the P. maxima extract treatment.

Conclusion

Plantago maxima water extract possessed antiadipogenic, antidiabetic, antiinflammatory, antioxidant activity, and normalized gut microflora in a rat model of diet-induced excessive adiposity due to a high content of biologically active compounds.

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Abbreviations

AC:

Abdominal circumference

AI:

Adiposity index

ANOVA:

Analysis of variance

AT:

Adipose tissue

BAAW:

n-Butanol–acetic acid–water

BMI:

Body mass index

CD:

Conjugated dienes

CFU:

Colony-forming units

CT:

Conjugated trienes

ELISA:

Enzyme-linked immunosorbent assay

HFD:

High-fat diet

IL-6:

Interleukine-6

LPS:

Lipopolysaccharide

MCP-1:

Macrophage chemoattractant protein-1

MDSA:

Microvessel density per specific area

PC:

Protein carbonyls

PMAT:

Parametrial adipose tissue

RPAT:

Retroperitoneal adipose tissue

STD:

Standard diet

TBARS:

Thiobarbituric acid-reactive substances

TC:

Thoracic circumference

TCC:

Total caloric consumption

TLC:

Total liquid consumption

TNF-α:

Tumor-necrosis factor-α

T-SH:

Total thiols

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Authors and Affiliations

  1. Department of Biochemistry, Orenburg State Medical Academy, Sovetskaya St., 6, 460014, Orenburg, Russia

    Alexey A. Tinkov, Olga N. Nemereshina, Elizaveta V. Popova & Alexandr A. Nikonorov

  2. Department of Pathologic Anatomy, Orenburg State Medical Academy, Sovetskaya St., 6, 460014, Orenburg, Russia

    Valentina S. Polyakova

  3. Laboratory of Cellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Pionerskaya St., 11, 460000, Orenburg, Russia

    Viktor A. Gritsenko

Authors
  1. Alexey A. Tinkov
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  2. Olga N. Nemereshina
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  3. Elizaveta V. Popova
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  5. Viktor A. Gritsenko
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Correspondence to Alexey A. Tinkov.

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Tinkov, A.A., Nemereshina, O.N., Popova, E.V. et al. Plantago maxima leaves extract inhibits adipogenic action of a high-fat diet in female Wistar rats. Eur J Nutr 53, 831–842 (2014). https://doi.org/10.1007/s00394-013-0587-6

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  • DOI: https://doi.org/10.1007/s00394-013-0587-6

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