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Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet

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Abstract

Background

Heart produces ATP through long-chain fatty acids beta oxidation.

Purpose

To analyze whether in ventricular myocardium, high-fat diet may modify the expression of proteins associated with energy metabolism before myocardial function was affected.

Methods

Wistar Kyoto rats were divided into two groups: (a) rats fed standard diet (control; n = 6) and (b) rats fed high-fat diet (HFD; n = 6). Proteins from left ventricles were analyzed by two-dimensional electrophoresis, mass spectrometry and Western blotting.

Results

Rats fed with HFD showed higher body weight, insulin, glucose, leptin and total cholesterol plasma levels as compared with those fed with standard diet. However, myocardial functional parameters were not different between them. The protein expression of 3-ketoacyl-CoA thiolase, acyl-CoA hydrolase mitochondrial precursor and enoyl-CoA hydratase, three long-chain fatty acid β-oxidation-related enzymes, and carnitine-O-palmitoyltransferase I was significantly higher in left ventricles from HFD rats. Protein expression of triosephosphate isomerase was higher in left ventricles from HFD rats than in those from control. Two α/β-enolase isotypes and glyceraldehyde-3-phosphate isomerase were significantly increased in HFD rats as compared with control. Pyruvate and lactate contents were similar in HFD and control groups. Expression of proteins associated with Krebs cycle and mitochondrial oxidative phosphorylation was higher in HFD rats.

Conclusions

Expression of proteins involved in left ventricle metabolic energy was enhanced before myocardial functionality was affected in rats fed with HFD. These findings may probably indicate higher cardiac energy requirement due to weight increase by HFD.

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Acknowledgments

Javier Modrego and José J. Zamorano-León are staff of Red Heracles. Petra J. Mateos-Cáceres is staff member of Fundación para la Investigación Biomédica, Hospital Clínico San Carlos. The authors thank Begoña Larrea and Miguel Dantart for their secretarial assistance. We also thank Sandra Ballesteros for her technical assistance. This work has been supported by Fondo de Investigaciones de la Seguridad Social (Redes Temáticas de Investigación Cooperativa, Red Heracles RD06/0009/0010) and Fundación Mutua Madrileña.

Conflict of interest

Authors declare no conflicts of interest.

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Authors and Affiliations

  1. Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Profesor Martín Lagos s/n, Madrid, Spain

    Javier Modrego, Jose J. Zamorano-León, Petra J. Mateos-Cáceres & Antonio J. López-Farré

  2. School of Medicine, Complutense University, Madrid, Spain

    Natalia de las Heras, Beatriz Martín-Fernández, Maria Valero-Muñoz & Vicente Lahera

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  1. Javier Modrego
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  2. Natalia de las Heras
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  3. Jose J. Zamorano-León
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  8. Antonio J. López-Farré
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Corresponding authors

Correspondence to Vicente Lahera or Antonio J. López-Farré.

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Modrego, J., de las Heras, N., Zamorano-León, J.J. et al. Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet. Eur J Nutr 52, 847–856 (2013). https://doi.org/10.1007/s00394-012-0392-7

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  • DOI: https://doi.org/10.1007/s00394-012-0392-7

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