Capsaicinoids are the active compounds in chili pepper. The present study investigated the effect of capsaicinoids on plasma lipids, functionality of aorta including atherosclerotic plaque development, cholesterol absorption biomarker, fecal sterol excretion, and gene expression of major receptors, enzymes, and transporters involved in cholesterol metabolism.
Hamsters were divided into five groups and fed a high-cholesterol diet containing 0 % (CON), 0.010 % (LD), 0.015 % (MD), 0.020 % (HD), and 0.030 % (VD) capsaicinoids, respectively, for 6 weeks. Plasma lipids were measured using the enzymatic kits, and the gene expression of transporters, enzymes, and receptors involved in cholesterol absorption and metabolism was quantified using the quantitative PCR. Endothelial function was assessed by measuring the acetylcholine-induced endothelium-dependent relaxations in aorta.
Capsaicinoids reduced plasma total cholesterol, non-high-density lipoprotein cholesterol, and triacylglycerols with high-density lipoprotein cholesterol being unaffected. All four experimental groups had a decrease in the atherosclerotic plaque compared with CON. Dietary capsaicinoids increased the fecal excretion of total acidic sterols possibly mediated by up-regulation of cholesterol 7α-hydroxylase and down-regulation of liver X receptor alpha. Plasma sterol analysis demonstrated that capsaicinoids decreased the ratio of plasma campesterol/cholesterol, suggesting they decreased cholesterol absorption. Capsaicinoids could improve the endothelium-dependent relaxations and reduce the endothelium-dependent contractions by inhibiting the gene expression of COX-2. However, no dose-dependent effect of capsaicinoids on these parameters was seen.
Capsaicinoids were beneficial in improving lipoprotein profile and aortic function in hamsters fed a high-cholesterol diet.
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Josse AR, Sherriffs SS, Holwerda AM, Andrews R, Staples AW, Phillips SM (2010) Effects of capsinoid ingestion on energy expenditure and lipid oxidation at rest and during exercise. Nutr Metab Lond 7:65
Luo XJ, Peng J, Li YJ (2010) Recent advances in the study on capsaicinoids and capsinoids. Eur J Pharmacol 650(1):1–7
Peng J, Li YJ (2010) The vanilloid receptor TRPV1: role in cardiovascular and gastrointestinal protection. Eur J Pharmacol 627:1–7
Rajput S, Mandal M (2012) Antitumor promoting potential of selected phytochemicals derived from spices: a review. Eur J Cancer Prev 21(2):205–215
Ahuja KD, Kunde DA, Ball MJ, Geraghty DP (2006) Effects of capsaicin, dihydrocapsaicin, and curcumin on copper-induced oxidation of human serum lipids. J Agric Food Chem 54:6436–6439
Manjunatha H, Srinivasan K (2007) Hypolipidemic and antioxidant effects of curcumin and capsaicin in high-fat-fed rats. Can J Physiol Pharmacol 85:588–596
Srinivasan MR, Sambaiah K, Satyananrayana MN, Rao MVL (1980) Influence of red pepper and capsaicin on growth, blood constituents and nitrogen balance in rats. Nutr Rep Int 21(3):455–467
Seo SJ, Kim J, Noh SK (2009) Effect of enteral capsaicin on the lymphatic absorption and fats in rat. Han’guk Sikp’um Yongyang Kwahak Hoechi 38:1712–1717
Chen ZY, Jiao R, Ma KY (2008) Cholesterol-lowering nutraceuticals and functional foods. J Agric Food Chem 56:8761–8773
Ostlund RE Jr, Lin X (2006) Regulation of cholesterol absorption by phytosterols. Curr Atheroscler Rep 8:487–491
Steinberg HO, Bayazeed B, Hook G, Johnson A, Cronin J, Baron AD (1997) Endothelial dysfunction is associated with cholesterol levels in the high normal range in humans. Circulation 96:3287–3293
Ng CH, Yao X, Huang Y, Chen ZY (2008) Oxidized cholesterol is more hypercholesterolemic and atherogenic than non-oxidized cholesterol in hamster. Br J Nutr 99:749–755
Warnick GR, Benderson J, Albers N (1982) Dextran sulfate-Mg2+ precipitation procedure for quantitation of high-density lipoprotein cholesterol. Clin Chem 28:1379–1388
Chan DC, Watts GF, Barrett PH, O’Neill FH, Redgrave TG, Thompson GR (2003) Relationships between cholesterol homoeostasis and triacylglycerol-rich lipoprotein remnant metabolism in the metabolic syndrome. Clin Sci Lond 104(4):383–388
Jiao R, Zhang Z, Yu H, Huang Y, Chen ZY (2010) Hypocholesterolemic activity of grape seed proanthocyanidin is mediated by enhancement of bile acid excretion and up-regulation of CYP7A1. J Nutr Biochem 21:1134–1139
Ma KY, Yang N, Jiao R, Peng C, Guan L, Huang Y, Chen ZY (2011) Dietary calcium decreases plasma cholesterol by down-regulation of intestinal NPC1L1 and MTP and up-regulation of CYP7A1 and ABCG 5/8 in hamsters. Mol Nutr Food Res 55:247–258
Liang YT, Wong WT, Guan L, Tian XY, Ma KY, Huang Y, Chen ZY (2011) Effect of phytosterols and their oxidation products on lipoprotein profiles and vascular function in hamster fed a high cholesterol diet. Atherosclerosis 219:124–133
Wong SL, Leung FP, Lau CW, Au CL, Yung LM, Yao X, Chen ZY, Vanhoutte PM, Gollasch M, Huang Y (2009) Cyclooxygenase-2-derived prostaglandin F2alpha mediates endothelium-dependent contractions in the aortae of hamsters with increased impact during aging. Circ Res 30:228–325
Srinivasan K, Sambaiah K (1991) The effect of spices on cholesterol 7 alpha-hydroxylase activity and on serum and hepatic cholesterol levels in the rat. Int J Vitam Nutr Res 61(4):364–369
Goodwin B, Watson MA, Kim H, Miao J, Kemper JK, Kliewer SA (2003) Differential regulation of rat and human CYP7A1 by the nuclear oxysterol receptor liver X receptor-α. Mol Endocrinol 17(3):386–394
Chiang JYL, Kimmel R, Stroup D (2001) Regulation of cholesterol 7α-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRα). Gene 262:257–265
Widlansky ME, Price DT, Gokce N, Eberhardt RT, Duffy SJ, Holbrook M, Maxwell C, Palmisano J, Keaney JF Jr, Morrow JD, Vita JA (2003) Short- and long-term COX-2 inhibition reverses endothelial dysfunction in patients with hypertension. Hypertension 42:310–315
Wong WT, Tian XY, Chen Y, Leung FP, Liu L, Lee HK, Ng CF, Xu A, Yao X, Vanhoutte PM, Tipoe GL, Huang Y (2010) Bone morphogenic protein-4 impairs endothelial function through oxidative stress-dependent cyclooxygenase-2 upregulation: implications on hypertension. Circ Res 107:984–991
Burleigh ME, Babaev VR, Yancey PG, Major AS, McCaleb JL, Oates JA, Morrow JD, Fazio S, Linton MF (2005) Cyclooxygenase-2 promotes early atherosclerotic lesion formation in ApoE-deficient and C57BL/6 mice. J Mol Cell Cardiol 39:443–452
Opinion of the Scientific Committee on Food on Capsaicin, European Commission Health & Consumer Protection Directorate-General: SCF/CS/FLAV/FLAVOUR/8 ADD1 Final. 28 Feb 2002
This project was supported by a grant from National Natural Science Foundation of China (NSFC).
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Liang, Y.T., Tian, XY., Chen, J.N. et al. Capsaicinoids lower plasma cholesterol and improve endothelial function in hamsters. Eur J Nutr 52, 379–388 (2013). https://doi.org/10.1007/s00394-012-0344-2
- Vascular reactivity