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Cardioprotective and hepatoprotective effects of ellagitannins from European oak bark (Quercus petraea L.) extract in rats

An Erratum to this article was published on 06 March 2012

Abstract

Background

Red wine contains many potentially bioactive polyphenols including resveratrol, catechins, anthocyanins and flavonoids as well as tannins derived from oak during maturation. This study examined the effects of a mixture of ellagitannins from oak bark (Quercus petraea L.) on cardiovascular, metabolic and liver changes in high-carbohydrate, high-fat diet–fed rats and in Spontaneously Hypertensive Rats (SHR).

Methods

First, 8-week-old male Wistar rats were divided into four groups and given either cornstarch diet, cornstarch diet + oak bark extract (0.5 mL/kg food), high-carbohydrate, high-fat diet or high-carbohydrate, high-fat diet + oak bark extract (0.5 mL/kg food) for 16 weeks. Oak bark extract was added to the diets for last 8 weeks of the feeding period. Secondly, SHR aged 42 weeks fed on standard chow diet were divided into two groups with and without oak bark extract treatment for 12 weeks (0.5 mL/kg food).

Results

The high-carbohydrate, high-fat diet induced signs of metabolic syndrome along with cardiovascular remodelling and non-alcoholic steatohepatitis. Oak bark extract attenuated the signs of metabolic syndrome in high-carbohydrate, high-fat diet–fed rats and improved the structure and function of the heart and the liver. SHR after oak bark extract treatment for 12 weeks showed lower systolic blood pressure, lower cardiac fibrosis and cardiac stiffness and improved vascular reactivity.

Conclusions

Oak bark extract containing ellagitannins improved cardiovascular, metabolic and liver parameters in these rat models of human disease, suggesting that part of the benefits attributed to red wine may be produced by these ellagitannins.

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Notes

  1. 1.

    Pure castalagin, vescalagin, grandinin and roburin E for use as standards were kindly provided by Professor Stéphane Quideau, European Institute of Chemistry and Biology, University of Bordeaux I, France.

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Acknowledgments

This study was supported by The Prince Charles Hospital Foundation, Brisbane, Australia. We thank Mr. Paul Addison, School of Biomedical Sciences, and Mr. Gary Wilson, School of Human Movement Studies, both at The University of Queensland, Australia, for their help with histopathological studies and plasma oxidant analyses, respectively. We also thank Dr. Fiona Campbell and Mr. Brian Bynon both at School of Veterinary Science, The University of Queensland, Australia, for their assistance with echocardiography and plasma analyses, respectively; Dr Thiruma Arumugam (The University of Queensland) for assistance with expression studies and Dr Kate Kauter (University of Southern Queensland) for assistance with C-reactive protein measurements. We also thank Prof. Stéphane Quideau, European Institute of Chemistry and Biology, University of Bordeaux I, France, and Dr. Daniel Jardine, Flinders University, Australia, for providing ellagitannin standards and ellagitannin analyses in the extract, respectively.

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No conflicts of interest.

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Correspondence to Lindsay Brown.

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Panchal, S.K., Brown, L. Cardioprotective and hepatoprotective effects of ellagitannins from European oak bark (Quercus petraea L.) extract in rats. Eur J Nutr 52, 397–408 (2013). https://doi.org/10.1007/s00394-011-0277-1

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Keywords

  • Obesity
  • Cardiovascular disease
  • Dyslipidaemia
  • Ellagitannins
  • Hypertension