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Mangiferin decreases inflammation and oxidative damage in rat brain after stress

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Abstract

Purpose

Stress exposure elicits neuroinflammation and oxidative damage in brain, and stress-related neurological and neuropsychiatric diseases have been associated with cell damage and death. Mangiferin (MAG) is a polyphenolic compound abundant in the stem bark of Mangifera indica L. with antioxidant and anti-inflammatory properties in different experimental settings. In this study, the capacity of MAG to prevent neuroinflammation and brain oxidative damage induced by stress exposure was investigated.

Methods

Young–adult male Wistar rats immobilized during 6 h were administered by oral gavage with increasing doses of MAG (15, 30, and 60 mg/Kg), respectively, 7 days before stress.

Results

Prior treatment with MAG prevented all of the following stress-induced effects: (1) increase in glucocorticoids (GCs) and interleukin-1β (IL-1β) plasma levels, (2) loss of redox balance and reduction in catalase brain levels, (3) increase in pro-inflammatory mediators, such as tumor necrosis factor alpha TNF-α and its receptor TNF-R1, nuclear factor-kappa B (NF-κB) and synthesis enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), (4) increase in lipid peroxidation.

Conclusions

These multifaceted protective effects suggest that MAG administration could be a new therapeutic strategy in neurological/neuropsychiatric pathologies in which hypothalamic/pituitary/adrenal (HPA) stress axis dysregulation, neuroinflammation, and oxidative damage take place in their pathophysiology.

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Acknowledgments

Supported by Spanish Ministry of Education (MEC, SAF07-63138), the Instituto de Salud Carlos III, Centro de Investigación en Red de Salud Mental, CIBERSAM, and Foundation Santander-UCM (GR 58/08). Lucía Márquez is recipient of a fellowship from Programme Alßan, European Commission.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Complutense University, 28040, Madrid, Spain

    Lucía Márquez, Borja García-Bueno, José L. M. Madrigal & Juan C. Leza

  2. Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain

    Borja García-Bueno, José L. M. Madrigal & Juan C. Leza

  3. Instituto de Investigación Sanitaria Hospital 12 de Octubre (I + 12), Madrid, Spain

    Borja García-Bueno, José L. M. Madrigal & Juan C. Leza

  4. Pharmaceutical Chemistry Center, Univ. La Habana, Habana, Cuba

    Lucía Márquez

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  1. Lucía Márquez
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  2. Borja García-Bueno
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  3. José L. M. Madrigal
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  4. Juan C. Leza
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Correspondence to Juan C. Leza.

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Márquez, L., García-Bueno, B., Madrigal, J.L.M. et al. Mangiferin decreases inflammation and oxidative damage in rat brain after stress. Eur J Nutr 51, 729–739 (2012). https://doi.org/10.1007/s00394-011-0252-x

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  • DOI: https://doi.org/10.1007/s00394-011-0252-x

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