Effect of consumption of tomato juice enriched with n-3 polyunsaturated fatty acids on the lipid profile, antioxidant biomarker status, and cardiovascular disease risk in healthy women
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We compared the effects of consumption of n-3 polyunsaturated fatty acids (PUFA)-enriched tomato juice versus plain tomato juice on the serum lipid profile and levels of biomarkers related to antioxidant status and cardiovascular disease (CVD) risk in women.
Eighteen healthy women participated in a 2-week intervention trial involving the daily intake of 500 mL of n-3 PUFA-enriched juice (n = 11) or plain tomato juice (n = 7). Each serving of enriched juice provided 250 mg of eicosapentaenoic acid (EPA) plus docosahexanoic acid (DHA). Both juices provided natural antioxidant compounds such as phenolics (181 mg) and lycopene (26.5 mg).
Intervention with the enriched juice had no effect on the lipid profile, and serum levels of triglycerides and cholesterol (total, LDL, and HDL) remained unchanged. The serum antioxidant status improved following juice intake, as revealed by an increase in total antioxidant capacity and a slight decrease in lipid peroxidation. The serum levels of homocysteine, a cardiovascular risk factor, decreased following n-3 PUFA-enriched juice consumption. A decrease in vascular adhesion molecule 1 (VCAM-1) levels was also noted after intake of either plain or enriched tomato juice, whereas intercellular adhesion molecule 1 (ICAM-1) levels only decreased following intake of the enriched juice.
Overall, stronger positive amelioration of CVD risk factors was observed following the intake of n-3 PUFA-enriched juice than after plain tomato juice consumption, which suggested a possible synergistic action between n-3 PUFAs and tomato antioxidants.
KeywordsCardiovascular diseases (CVD) PUFAs Tomato juice Antioxidants Homocysteine Lipid oxidation VCAM-1 ICAM-1
We thank to Hero España S.A. and Denomega Nutritional Oil for providing the samples and ingredients of this study. The authors are grateful to the Ministry of Education and Science of the Spanish Government for the project AGL 2006-26965-E, and to the Fundación Seneca of the Murcia Regional Government for the project 05774/PI/07.
- 2.Leal J, Luengo-Fernandez R, Gray A, Petersen S, Rayner M (2006) ACE enzyme inhibitors, anti-arrhytmic, anti-hypertensive, anti-atherosclerotic, anti-inflammatory, cytoprotective. Economic burden of cardiovascular diseases in the enlarged European Union. Eur Heart J 27(13):1610–1619CrossRefGoogle Scholar
- 3.Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, De Simone G, Ferguson TB, Ford E, Furie K, Gillespie C, Go A, Greenlund K, Haase N, Hailpern S, Ho PM, Howard V, Kissela B, Kittner S, Lackland D, Lisabeth L, Marelli A, McDermott MM, Meigs J, Mozaffarian D, Mussolino M, Nichol G, Roger V, Rosamond W, Sacco R, Sorlie P, Stafford R, Thom T, Wasserthiel-Smoller S, Wong ND, Wylie-Rosett J (2010) Heart disease and stroke statistics-2010 Update. A report from the American Heart Association. Circulation 121:e46–e215CrossRefGoogle Scholar
- 5.Bhatnagar D, Durrington PN (2003) Omega-3 fatty acids: their role in the prevention and treatment of atherosclerosis related risk factors and complications. Int J Clin Pract 57:305–314Google Scholar
- 6.Nishida C, Uauy R, Kumanyika S, Shetty P (2004) The Joint WHO/FAO Expert Consultation on diet, nutrition and the prevention of chronic diseases: process, product and policy implications. Public Health Nutr 7:245–250Google Scholar
- 10.Patel JV, Tracey I, Hughes EA, Lip GYH (2000) Omega-3 polyunsaturated fatty acids: a necessity for a comprehensive secondary prevention strategy. Vasc Health Risk Manag 5:801–810Google Scholar
- 12.Vrablik M, Prusikova M, Snejdrlova M, Zlatohlavek L (2009) Omega-3 fatty acids and cardiovascular disease risk. Do we understand the relationship? Physiol Res 58((Suppl 1)):S19–S26Google Scholar
- 14.World Health Organization (1985) Energy and protein requirements. Technical Report Series, No. 724. Report of a Joint FAO/WHO/UNU Expert Consultation. World Health Organization. GenevaGoogle Scholar
- 15.Baghurst K (2005) Executive summary of nutrient reference values for Australia and New Zealand including recommended dietary intakes. Commonwealth department of health and ageing, Australia. Ministryof health, New Zealand. National Health and Medical Research. Council. http://www.nhmrc.gov.au/publications/synopses/n35syn.htm
- 24.Rao AV (2002) Lycopene, tomatoes, and the prevention of coronary heart disease. Exp Biol Med 227:908–913Google Scholar
- 29.Castelli WP (1988) Cholesterol and lipids in the risk of coronary artery disease. The Framingham Heart Study. Can J Cardiol 4(Suppl A):5A–10AGoogle Scholar
- 36.Baró L, Fonollá J, Peña JL, Martínez-Férez A, Lucena A, Jiménez J, Boza JJ, López-Huertas E (2003) n-3 fatty acids plus oleic acid and vitamin supplemented milk consumption reduces total and LDL cholesterol, homocysteine and levels of endothelial adhesion molecules in healthy humans. Clin Nutr 22:175–182CrossRefGoogle Scholar
- 37.Carrero JJ, Fonollá J, Mart JL, Jimenez J, Boza JJ, Lopez-Huertas E (2007) Intake of fish oil, oleic acid, folic acid, and vitamins B-6 and E for 1 year decreases plasma-C-reactive protein and reduces coronary heart disease risk factors in male patients in a cardiac rehabilitation program. J Nutr 137:384–390Google Scholar
- 43.López-García E, Schulze MB, Manson JE, Meigs JB, Albert CM, Rifai N, Willett WC, Hu FB (2004) Consumption of (n-3) fatty acids is related to plasma biomarkers of inflammation and endothelial activation in women. J Nutr 134:1086–1811Google Scholar
- 49.Ueland PM, Refsum H, Beresford SA, Wollset SE (2000) The controversy over homocysteine and cardiovascular risk. Am J Clin Nutr 72:324–332Google Scholar
- 52.Grundt H, Nilsen DWT, Mansoor MA, Hetland O, Nordoy A (2003) Reduction in homocysteine by n-3 polyunsaturated fatty acids after 1 year in a randomized double-blind study following an acute myocardial infarction: no effect on endothelial adhesion properties. Pathophysiol Haemost Thromb 33:88–95CrossRefGoogle Scholar
- 55.Liu X, Qu D, He F, Lu Q, Wang J, Cai D (2007) Effect of lycopene on the vascular endothelial function and expression of inflammatory agents in hyperhomocysteinemic rats. Asia Pac J Clin Nutr 16:244–248Google Scholar
- 56.Blum A, Moni M, Khazim K, Peleg A, Blum N (2007) Tomato-rich (Mediterranean) diet does not modify inflammatory markers. Clin Invest Med 30(2):E70–E74Google Scholar