Abstract
Purpose
Fructose feeding induces a moderate increase in blood pressure, insulin resistance, and hyperinsulinemia. This study investigated the role of α1B-adrenoceptor subtype in the control of renal hemodynamic responses to exogenously administered angiotensin II (Ang II) and a set of adrenergic agonists in a model of high fructose-fed rats.
Methods
Sprague–Dawley rats were fed for 8 weeks with 20% fructose in drinking water (FFR). The renal cortical vasoconstriction to noradrenaline (NA), phenylephrine (PE), methoxamine (ME) and Ang II in the presence and absence of chloroethylclonidine (CEC) (α1B-adrenoceptor antagonist) was determined. Data, mean ± SEM or SD were subjected to ANOVA with significance at p < .05.
Results
FFR showed significant increase in the systolic blood pressure, plasma glucose, and insulin levels when compared to control. FFR expressed reduced renal cortical vascular sensitivity to NA, PE, ME, and Ang II. Furthermore, renal cortical vasoconstriction response to NA, PE, ME, and Ang II was blunted in the presence of CEC in control. While in FFR, renal cortical vasoconstriction to NA, PE, and ME was enhanced by CEC. Renal cortical vasoconstriction to Ang II in FFR was reduced in the presence of CEC.
Conclusions
In the presence of a hyperinsulinemic state resulting from chronic and high fructose feeding, an attenuated AT1 and α1-adrenoceptors response to Ang II and adrenergic stimuli respectively, is expected. In addition, α1B-adrenoceptor is the functional subtype that mediates renal cortical vasoconstriction in control rat, while high fructose feeding did influence the functionality of α1B-adrenoceptor in mediating the renal cortical hemodynamic changes.
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Acknowledgments
Mohammed H. Abdulla is a recipient of Vice Chancellor’s Award, USM fellowship from institute of graduate studies (IPS) of Universiti Sains Malaysia, Janssen Pharmaceuticals Award of best research thesis 2009 and the American Physiological Society Early Career Physiologist Award 2010, all gratefully acknowledged.
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Abdulla, M.H., Sattar, M.A., Abdullah, N.A. et al. The contribution of α1B-adrenoceptor subtype in the renal vasculature of fructose-fed Sprague–Dawley rats. Eur J Nutr 50, 251–260 (2011). https://doi.org/10.1007/s00394-010-0133-8
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DOI: https://doi.org/10.1007/s00394-010-0133-8