Randomised, double-blind and placebo-controlled study using new probiotic lactobacilli for strengthening the body immune defence against viral infections
- 1.2k Downloads
The aim of this study was to investigate whether consumption of Lactobacillus plantarum HEAL 9 (DSM 15312) and Lactobacillus paracasei 8700:2 (DSM 13434) could affect naturally acquired common cold infections in healthy subjects.
A randomised, parallel, double-blind placebo-controlled study was performed to investigate whether intake of this probiotic mixture could reduce the risk of common cold episodes, number of days with common cold symptoms, frequency and severity of symptoms, and cellular immune response in common cold infections. A total of 272 subjects were supplemented daily with either 109 cfu (colony forming units) of probiotics (N = 135) or control (N = 137) for a 12-week period.
The incidence of acquiring one or more common cold episode was reduced from 67% in the control group to 55% in the probiotic group (p < 0.05). Also, the number of days with common cold symptoms were significantly (p < 0.05) reduced from 8.6 days in the control group to 6.2 days, in the probiotic group, during the 12-week period. The total symptom score was reduced during the study period from a mean of 44.4 for the control group to 33.6 for the probiotic group. The reduction in pharyngeal symptoms was significant (p < 0.05). In addition, the proliferation of B lymphocytes was significantly counteracted in the probiotic group (p < 0.05) in comparison with the control group.
In conclusion, intake of the probiotic strains Lactobacillus plantarum HEAL 9 (DSM 15312) and Lactobacillus paracasei 8700:2 (DSM 13434) reduces the risk of acquiring common cold infections.
KeywordsProbiotic Immune defence Common cold Lactobacillus plantarum Lactobacillus paracasei DSM 15312 DSM 13434
Conflict of interest
The authors are employees at Probi AB, and the study was funded by Probi AB and VINNOVA (The Swedish Governmental Agency for Innovation Systems).
- 2.FAO/WHO Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf
- 5.de Vrese M, Winkler P, Rautenberg P, Harder T, Noah C, Laue C, Ott S, Hampe J, Schreiber S, Heller K, Schrezenmeir J (2005) Effect of Lactobacillus gasseri PA 16/8, Bifidobacterium longum SP 07/3, B. bifidum MF 20/5 on common cold episodes: a double blind, randomized, controlled trial. Clin Nutr 24(4):481–491CrossRefGoogle Scholar
- 6.de Vrese M, Winkler P, Rautenberg P, Harder T, Noah C, Laue C, Ott S, Hampe J, Schreiber S, Heller K, Schrezenmeir J (2006) Probiotic bacteria reduced duration and severity but not the incidence of common cold episodes in a double blind, randomized, controlled trial. Vaccine 24(44–46):6670–6674Google Scholar
- 7.Winkler P, de Vrese M, Ch Laue, Schrezenmeir J (2005) Effect of a dietary supplement containing probiotic bacteria plus vitamins and minerals on common cold infections and cellular immune parameters. Int J Clin Pharmacol Ther 43(7):318–326Google Scholar
- 8.Lavasani S, Dzhambazov B, Nouri M, Fåk F, Buske S, Molin G, Thorlacius H, Alenfall J, Jeppsson B, Weström B (2010) A novel probiotic mixture exerts a therapeutic effect on experimental autoimmune encephalomyelitis mediated by IL-10 producing regulatory T cells. PLoS One 5(2):1–11CrossRefGoogle Scholar
- 9.Antonsson M, Molin G, Probi AB (2003) New strains of Lactobacillus paracasei, patent EP1313837. Available at http://v3.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=1313837B1&KC=B1&FT=D&date=20051214&DB=EPODOC&locale=se_SE
- 10.Molin G, Ahrné S, Jeppsson B, Vasquez A, Berggren A, Probi AB (2006) WO patent application 2006038869. Available at http://v3.espacenet.com/publicationDetails/biblio?CC=WO&NR=2006038869A1&KC=A1&FT=D&date=20060413&DB=EPODOC&locale=se_SE
- 18.Macfarlane GT, Steed H, Macfarlane S (2008) Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics. J Appl Microbiol 104(2):305–344Google Scholar