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Antiobesity effect of polyphenolic compounds from molokheiya (Corchorus olitorius L.) leaves in LDL receptor-deficient mice

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Abstract

Background

Dietary supplementation with polyphenolic compounds is associated with reduced diet-induced obesity and metabolic disorders in humans. The antioxidative properties of polyphenolic compounds contribute to their antiobesity effect in animal experiments and human studies.

Aim

The aim of the study was to investigate the antiobesity effect of polyphenolic compounds from molokheiya leaves in LDLR-/- mice fed high-fat diet and to elucidate the mechanism of this effect.

Methods

Three groups of LDLR-/- mice were fed with a high-fat diet, supplemented with 0% (control), 1 or 3% molokheiya leaf powder (MLP). Gene expression in the liver associated with lipid and glucose metabolism was analyzed, and physical parameters and blood biochemistry were determined.

Results

Compared to controls, mice body weight gain (P = 0.003), liver weight (P = 0.001) and liver triglyceride levels (P = 0.005) were significantly lower in the two MLP groups. Epididymal adipose tissue weight (P = 0.003) was reduced in the 3% MLP group. Liver tissue gene expression of gp91phox (NOX2), involved in oxidative stress, was significantly down-regulated (P = 0.005), and PPARα and CPT1A, related to the activation of β-oxidation, were significantly up-regulated (P = 0.025 and 0.006, respectively) in the 3% MLP group compared to the control group.

Conclusions

Our results demonstrate an antiobesity effect of polyphenolic compounds from molokheiya leaves and that this effect is associated with reduction in oxidative stress and enhancement of β-oxidation in the liver. Consumption of molokheiya leaves may be beneficial for preventing diet-induced obesity.

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Abbreviations

ACOX1:

Acyl-coenzyme A oxidase 1

CPT1A:

Carnitine palmitoyl transferase 1A

Ehhadh:

Enoyl-coenzyme A hydratase/3-hydroxyacyl coenzyme A dehydrogenase

FAS:

Fatty acid synthase

GK:

Glucokinase

LDLR-/-:

Low-density lipoprotein receptor-deficient

MLP:

Molokheiya leaf powder

PPARα:

Peroxisome proliferator activated receptor alpha

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Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology to M. Yamasaki and Grants for Scientific Research from Shimane Prefecture and collaborated with Shimane Institute for Industrial Technology and Izumoya Co. Ltd. We thank Dr. Jeff Burgess for checking our manuscript.

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Authors and Affiliations

  1. Department of Environmental and Preventive Medicine, Shimane University School of Medicine, 89-1 Enya-cho, Izumo City, Shimane, 693-8501, Japan

    Li Wang, Masayuki Yamasaki, Xufeng Sun & Kuninori Shiwaku

  2. Shimane Institute for Industrial Technology, 1 Hokuryo-cho, Matsue City, Shimane, 690-0816, Japan

    Takuya Katsube & Yukikazu Yamasaki

Authors
  1. Li Wang
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  2. Masayuki Yamasaki
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  3. Takuya Katsube
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  5. Yukikazu Yamasaki
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Correspondence to Masayuki Yamasaki.

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Wang, L., Yamasaki, M., Katsube, T. et al. Antiobesity effect of polyphenolic compounds from molokheiya (Corchorus olitorius L.) leaves in LDL receptor-deficient mice. Eur J Nutr 50, 127–133 (2011). https://doi.org/10.1007/s00394-010-0122-y

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  • DOI: https://doi.org/10.1007/s00394-010-0122-y

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