Abstract
Background
The nutritional composition of the dietary intake could produce specific effects on metabolic variables and inflammatory marker concentrations. This study assessed the effects of two hypocaloric diets (legume-restricted- vs. legume-based diet) on metabolic and inflammatory changes, accompanying weight loss.
Methods
Thirty obese subjects (17 M/13F; BMI: 32.5 ± 4.5 kg/m2; 36 ± 8 years) were randomly assigned to one of the following hypocaloric treatments (8 weeks): Calorie-restricted legume-free diet (Control: C-diet) or calorie-restricted legume-based diet (L-diet), prescribing 4 weekly different cooked-servings (160–235 g) of lentils, chickpeas, peas or beans. Body composition, blood pressure (BP), blood biochemical and inflammatory marker concentrations as well as dietary intake were measured at baseline and after the nutritional intervention.
Results
The L-diet achieved a greater body weight loss, when compared to the C-diet (−7.8 ± 2.9% vs. −5.3 ± 2.7%; p = 0.024). Total and LDL cholesterol levels and systolic BP were improved only when consuming the L-diet (p < 0.05). L-diet also resulted in a significant higher reduction in C-reactive protein (CRP) and complement C3 (C3) concentrations (p < 0.05), compared to baseline and C-diet values. Interestingly, the reduction in the concentrations of CRP and C3 remained significantly higher to L-diet group, after adjusting by weight loss (p < 0.05). In addition, the reduction (%) in CRP concentrations was positively associated with decreases (%) in systolic BP and total cholesterol concentration specifically in the L-diet group, independent from weight loss (p < 0.05).
Conclusion
The consumption of legumes (4 servings/week) within a hypocaloric diet resulted in a specific reduction in proinflammatory markers, such as CRP and C3 and a clinically significant improvement of some metabolic features (lipid profile and BP) in overweight/ obese subjects, which were in some cases independent from weight loss.
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References
Puchau B, Zulet MA, González de Echavarri A, Navarro-Blasco I, Martínez JA (2009) Selenium intake reduces serum C3, an early marker of metabolic syndrome manifestations, in healthy young adults. Eur J Clin Nutr 63:858–864
Wald DS, Wald NJ, Morris JK, Law M (2006) Folic acid, homocysteine, and cardiovascular disease: judging causality in the face of inconclusive trial evidence. BMJ 333:1114–1117
Zulet MA, Puchau B, Navarro C, Marti A, Martínez JA (2007) Inflammatory biomarkers: the link between obesity and associated pathologies. Nutr Hosp 22:511–527
Hermsdorff HHM, Monteiro JB (2004) Visceral, subcutaneous or intramuscular fat: where is the problem? Arq Bras Endocrinol Metabol 48:803–811
Rondinone CM (2006) Adipocyte-derived hormones, cytokines, and mediators. Endocrine 29:81–90
Van Gaal LF, Mertens IL, De Block CE (2006) Mechanisms linking obesity with cardiovascular disease. Nature 444:875–880
Hermsdorff HHM, Zulet MA, Abete I, Martínez JA (2009) Discriminated benefits of a Mediterranean dietary pattern within a hypocaloric diet program on plasma RBP4 concentrations and other inflammatory markers in obese subjects. Endocrine 36:445–451
Hermsdorff HHM, Zulet MA, Bressan J, Martínez-González MA, Martínez JA (2009) Impact of caloric restriction and Mediterranean food-based diets on inflammation accompanying obesity and metabolic syndrome features. Obe Metab 5:69–77
Bazzano LA, Thompson AM, Tees MT, Nguyen CH, Winham DM (2009) Non-soy legume consumption lowers cholesterol levels: a meta-analysis of randomized controlled trials. Nutr Metab Cardiovasc Dis. doi:10.1016/j.numecd.2009.08.012
Trinidad TP, Mallillin AC, Loyola AS, Sagum RS, Encabo RR (2010) The potential health benefits of legumes as a good source of dietary fibre. Br J Nutr 103:569–574
Zulet MA, Martínez JA (2001) Mediterranean diet: legumes and cholesterolemia. Rev Chil Nutr 28:312–320
Mitchell DC, Lawrence FR, Hartman TJ, Curran JM (2009) Consumption of dry beans, peas, and lentils could improve diet quality in the US population. J Am Diet Assoc 109:909–913
Papanikolaou Y, Fulgoni VL 3rd (2008) Bean consumption is associated with greater nutrient intake, reduced systolic blood pressure, lower body weight, and a smaller waist circumference in adults: results from the national health and nutrition examination survey 1999–2002. J Am Coll Nutr 27:569–576
Pittaway JK, Robertson IK, Ball MJ (2008) Chickpeas may influence fatty acid and fiber intake in an ad libitum diet, leading to small improvements in serum lipid profile and glycemic control. J Am Diet Assoc 108:1009–1013
Venn BJ, Mann JI (2004) Cereal grains, legumes and diabetes. Eur J Clin Nutr 58:1443–1461
Abete I, Parra D, Martinez JA (2009) Legume-, fish-, or high-protein-based hypocaloric diets: effects on weight loss and mitochondrial oxidation in obese men. J Med Food 12:100–108
Abete I, Parra D, Martinez JA (2008) Energy-restricted diets based on a distinct food selection affecting the glycemic index induce different weight loss and oxidative response. Clin Nutr 27:545–551
Crujeiras AB, Parra D, Abete I, Martínez JA (2007) A hypocaloric diet enriched in legumes specifically mitigates lipid peroxidation in obese subjects. Free Radic Res 41:498–506
WHO (2000) World Health Organization. Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep 894:1–253
Aranceta J (2001) Spanish food patterns. Public Health Nutr 4:1399–1402
Dapcich V, Salvador-Castell G, Ribas-Barba L, Pérez-Rodrigo C, Aranceta J, Serra-Majem L (2004) Guía de la alimentación saludable. SENC. Sociedad Española de Nutrición Comunitaria, Madrid. [Guide for a health diet]
Abete I, Parra D, De Morentin BM, Alfredo Martinez J (2009) Effects of two energy-restricted diets differing in the carbohydrate/protein ratio on weight loss and oxidative changes of obese men. Int J Food Sci Nutr 60(Suppl 3):1–13
Martínez-González MA, López-Fontana C, Varo JJ, Sánchez-Villegas A, Martínez JA (2005) Validation of the Spanish version of the physical activity questionnaire used in the nurses’ health study and the health professionals’ follow-up study. Public Health Nutr 8:920–927
Pérez S, Parra MD, Martínez de Morentin B, Rodríguez MC, Martínez JA (2005) Evaluación de la variabilidad intraindividual de la medida de composición corporal mediante bioimpedancia en voluntarias sanas y su relación con el índice de masa corporal y el pliegue tricipital. Enferm Clin 15:307–314
Labayen I, Diez N, Parra MD, González A, Martínez JA (2004) Time-course changes in macronutrient metabolism induced by a nutritionally balanced low-calorie diet in obese women. Int J Food Sci Nutr 55:27–35
Gibson R (2005) Principles of nutritional assessment. Oxford University Press, New York
Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28:412–419
Sharman MJ, Volek JS (2004) Weight loss leads to reductions in inflammatory biomarkers after a very-low-carbohydrate diet and a low-fat diet in overweight men. Clin Sci (Lond) 107:365–369
Dean AG, Sullivan KM, Soe MM (2008) OpenEpi source epidemiology statistics for public health, version 2.3. www.OpenEpi.com, update 2008/20/05
Willett WC (1998) Nutritional epidemiology. Oxford University Press, New York
Abete I, Parra D, Crujeiras AB, Goyenechea E, Martinez JA (2008) Specific insulin sensitivity and leptin responses to a nutritional treatment of obesity via a combination of energy restriction and fatty fish intake. J Hum Nutr Diet 21:591–600
Crujeiras AB, Parra D, Milagro FI et al (2008) Differential expression of oxidative stress and inflammation related genes in peripheral blood mononuclear cells in response to a low-calorie diet: a nutrigenomics study. Omics 12:251–261
Goyenechea E, Parra D, Crujeiras AB, Abete I, Martinez JA (2009) A nutrigenomic inflammation-related PBMC-based approach to predict the weight-loss regain in obese subjects. Ann Nutr Metab 54:43–51
Shai I, Schwarzfuchs D, Henkin Y et al (2008) Weight loss with a low-carbohydrate, Mediterranean, or low-fat diet. N Engl J Med 359:229–241
Hartman TJ, Albert PS, Zhang Z et al (2010) Consumption of a legume-enriched, low-glycemic index diet is associated with biomarkers of insulin resistance and inflammation among men at risk for colorectal cancer. J Nutr 140:60–67
Rochfort S, Panozzo J (2007) Phytochemicals for health, the role of pulses. J Agric Food Chem 55:7981–7994
Ma Y, Griffith JA, Chasan-Taber L et al (2006) Association between dietary fiber and serum C-reactive protein. Am J Clin Nutr 83:760–766
King DE, Egan BM, Woolson RF, Mainous AG 3rd, Al-Solaiman Y, Jesri A (2007) Effect of a high-fiber diet vs a fiber-supplemented diet on C-reactive protein level. Arch Intern Med 167:502–506
Bo S, Durazzo M, Guidi S et al (2006) Dietary magnesium and fiber intakes and inflammatory and metabolic indicators in middle-aged subjects from a population-based cohort. Am J Clin Nutr 84:1062–1069
Song Y, Li TY, van Dam RM, Manson JE, Hu FB (2007) Magnesium intake and plasma concentrations of markers of systemic inflammation and endothelial dysfunction in women. Am J Clin Nutr 85:1068–1074
Rizkalla SW, Bellisle F, Slama G (2002) Health benefits of low glycaemic index foods, such as pulses, in diabetic patients and healthy individuals. Br J Nutr 88(Suppl 3):S255–S262
Bazzano LA, He J, Ogden LG et al (2001) Legume consumption and risk of coronary heart disease in US men and women: NHANES I Epidemiologic Follow-up Study. Arch Intern Med 161:2573–2578
Mallillin AC, Trinidad TP, Raterta R, Dagbay K, Loyola AS (2008) Dietary fibre and fermentability characteristics of root crops and legumes. Br J Nutr 100:485–488
Martínez JA, Margareto J, Marti A, Milagro FI, Larrarte E, Moreno Aliaga MJ (2001) Resistin overexpression is induced by a beta3 adrenergic agonist in diet-related overweightness. J Physiol Biochem 57:287–288
Acknowledgments
We wish to thank our physician (Blanca E. Martínez de Morentin), our nurse (Salomé Pérez) and our technicians (Ana Lorente and Verónica Ciaurriz), for excellent technical assistance. This work was supported by Línea Especial about Nutrition, Obesity and Health (University of Navarra LE/97) and by Health Department of the Government of Navarra (22/2007). The Capes Foundation-Ministry of Education of Brazil also provided research grant to H. H. M. Hermsdorff (no 3756/05-0).
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The authors declared no conflict of interest.
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Hermsdorff, H.H.M., Zulet, M.Á., Abete, I. et al. A legume-based hypocaloric diet reduces proinflammatory status and improves metabolic features in overweight/obese subjects. Eur J Nutr 50, 61–69 (2011). https://doi.org/10.1007/s00394-010-0115-x
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DOI: https://doi.org/10.1007/s00394-010-0115-x