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Does ascorbic acid supplementation affect iron bioavailability in rats fed micronized dispersible ferric pyrophosphate fortified fruit juice?

Abstract

Background and aims

Food iron (Fe) fortification is an adequate approach for preventing Fe-deficiency anemia. Poorly water-soluble Fe compounds have good sensory attributes but low bioavailability. The reduction of the particle size of Fe fortificants and the addition of ascorbic acid might increase the bioavailability of low-soluble compounds. The present work aims to compare the Fe absorption and bioavailability of micronized dispersible ferric pyrophosphate (MDFP) (poorly soluble) to ferrous sufate (FS) (highly soluble) added to a fruit juice in presence or absence of ascorbic acid (AA) by using the hemoglobin repletion assay in rats.

Methods

After a hemoglobin depletion period, four fruit juices comprised of (1) FS, (2) MDFP, (3) FS + AA, (4) MDFP + AA were produced and administered to a different group of rats (n = 18) over 21 days. During the repletion period, Fe balance, hemoglobin regeneration efficiency (HRE), relative bioavailability (RBV) and Fe tissue content were determined in the short, medium and long term.

Results

Fe absorption and bioavailability showed no significant differences between fortifying the fruit juice with FS or MDFP. The addition of AA to the juice enhanced Fe absorption during the long-term balance study within the same Fe source. HRE and Fe utilization increased after AA addition in both FS and MDFP groups in every period.

Conclusion

Fe absorption and bioavailability from MDFP were comparable to FS added to a fruit juice in rats. Further, the addition of AA enhanced Fe absorption in the long term, as well as Fe bioavailability throughout the repletion period regardless of the Fe source employed.

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References

  1. 1.

    Buchowski MS, Mahoney AW, Kalpalathika PV (1989) Nonheme iron absorption, apparent iron absorption and Hb regeneration efficiency in anemic and normal rats fed with dietary heme and nonheme iron at various levels. Nutr Res 9:773–783

    Article  CAS  Google Scholar 

  2. 2.

    Davidsson L, Walczyk T, Zavaleta N, Hurrell RF (2001) Improving iron absorption from a Peruvian school breakfast meal by adding ascorbic acid or Na2EDTA. Am J Clin Nutr 73:283–287

    CAS  Google Scholar 

  3. 3.

    Derman DP, Bothwell TH, Torrance JD, Bezwoda WR, MacPhail AP, Kew MC, Sayers MH, Disler PB, Charlton RW (1980) Iron absorption from maize (Zea Mays) and sorghum (Sorghum vulgare) beer. Br J Nutr 43:271–279

    Article  CAS  Google Scholar 

  4. 4.

    Fairweather-Tait SJ, Piper Z, Fatemi SJ, Moore GR (1991) The effect of tea on iron and aluminium metabolism in the rat. Br J Nutr 65:61–68

    Article  CAS  Google Scholar 

  5. 5.

    Fidler MC, Walczyk T, Davidsson L, Zeder C, Sakaguchi N, Juneja LR, Hurrell RF (2004) A micronised, dispersible ferric pyrophosphate with high relative bioavailability in man. Br J Nutr 91:107–112

    Article  CAS  Google Scholar 

  6. 6.

    Fidler MC, Davidsson L, Zeder C, Walczyk T, Marti L, Hurrell RF (2004) Effect of ascorbic acid and particle size on iron absorption from ferric pyrophosphate in adult women. Int J Vitam Nutr Res 74:294–300

    Article  CAS  Google Scholar 

  7. 7.

    Fischer JG, Glauert HP, Yin T, Sweeney-Reeves ML, Larmonier N, Black MC (2002) Moderate iron overload enhances lipid peroxidation in livers of rats, but does not affect NF-KappaB activation induced by the peroxisome proliferator. J Nutr 132:2525–2531

    CAS  Google Scholar 

  8. 8.

    Forbes AL, Adams CE, Arnaud MJ, Chichester CO, Cook JD, Harrinson BN, Hurrell RF, Kahn SG, Morris ER, Tanner JT, Whittaker P (1989) Comparison of in vitro, animal, and clinical determinations of iron bioavailability: International Nutritional Anemia Consultative Group Task Force report on iron bioavailability. Am J Clin Nutr 49:225–228

    CAS  Google Scholar 

  9. 9.

    Hallberg L, Brune M, Rossander L (1986) Low bioavailability of carbonyl iron in man: studies on iron fortification of wheat flour. Am J Clin Nutr 43:59–67

    CAS  Google Scholar 

  10. 10.

    Haro-Vicente JF, Martínez-Graciá MC, Ros G (2006) Optimisation of in vitro measurement of available iron from different fortificants in fruit juices. Food Chem 98:639–648

    Article  CAS  Google Scholar 

  11. 11.

    Hurrell RF (1997) Preventing iron deficiency through food fortification. Nutr Rev 55:210–222

    CAS  Google Scholar 

  12. 12.

    Hurrell RF (2002) Fortification: overcoming technical and practical barriers. J Nutr 132:806S–812S

    CAS  Google Scholar 

  13. 13.

    Hurrell RF (2002) How to ensure adequate iron absorption from iron fortified food. Nutr Rev 60:S7–S15

    Article  Google Scholar 

  14. 14.

    Hurrell RF, Reddy MB, Burri J, Cook JD (2000) An evaluation of EDTA compounds for iron fortification of cereals-based foods. Br J Nutr 84:903–910

    CAS  Google Scholar 

  15. 15.

    Hurrell RF, Lynch S, Bothwell T, Cori H, Glahn R, Hertrampf E, Kratky Z, Miller D, Rodenstein M, Streekstra H, Teucher B, Turner E, Yeung CK, Zimmermann MB (2004) Enhancing the absorption of fortification iron. Int J Vitam Nutr Res 74(6):387–401

    Article  CAS  Google Scholar 

  16. 16.

    Klopotek Y, Otto K, Böhm V (2005) Processing strawberries to different products alters contents of vitamin C, total phenolics, total anthocyanins, and antioxidant capacity. J Agric Food Chem 53:5640–5656

    Article  CAS  Google Scholar 

  17. 17.

    Lynch SR, Stoltzfus RJ (2003) Iron and ascorbic acid: proposed fortification levels and recommended iron compound. J Nutr 133:2978S–2984S

    Google Scholar 

  18. 18.

    MacPhail AP, Patel RC, Bothwell TH, Lamparelli RD (1994) EDTA and the absorption of iron from food. Am J Clin Nutr 59:644–648

    CAS  Google Scholar 

  19. 19.

    Mahoney AW, Hendricks DG (1982) Efficiency of Hb regeneration as a method of assessing iron bioavailability in foods products. In: Kies, C (ed) Nutritional bioavailability of iron) Am Chem Soc, Washington, DC, pp 1–11

  20. 20.

    Moretti D, Zimmermann MB, Wegmüller R, Walczyk T, Zeder C, Hurrell RF (2006) Iron status and food matrix strongly affect the relative bioavailability of ferric pyrophosphate in humans. Am J Clin Nutr 83:632–638

    CAS  Google Scholar 

  21. 21.

    Nambu H, Nakata K, Sakaguchi N, Yamazaki Y, inventors; Taiyo Kagaku Ltd, assignee. Mineral composition. US patent 6074675. June 13, 2000

  22. 22.

    Sakaguchi N, Rao TP, Nakata K, Nanbu H, Juneja LR (2004) Iron absorption and bioavailability in rats of micronized dispersible ferric pyrophosphate. Int J Vitam Nutr Res 74:3–9

    Article  CAS  Google Scholar 

  23. 23.

    Sanchez-Morito N, Planells E, Aranda P, Llopis J (2000) Influence of magnesium deficiency on the bioavailability and tissue distribution of iron in the rat. J Nutr Biochem 11(2):103–108

    Article  CAS  Google Scholar 

  24. 24.

    Sayers MH, Lynch SR, Charlton RW, Bothwell TH, Walker RB, Mayet F (1974) Iron absorption from rice meals cooked with fortified salt containing ferrous sulfate and ascorbic acid. Br J Nutr 31:367–375

    Article  CAS  Google Scholar 

  25. 25.

    Shaw N, Liu Y (2000) Bioavailability of iron from purple laver (porphyrya spp.) estimated in a rats hemoglobin regeneration bioassay. J Agric Food Chem 48:1734–1737

    Article  CAS  Google Scholar 

  26. 26.

    Swain JH, Newman SM, Hunt JR (2003) Bioavailability of elemental iron powders to rats is less than bakery-grade FS and predicted by iron solubility and particle surface area. J Nutr 133:3546–3552

    CAS  Google Scholar 

  27. 27.

    Tarng DC, Huang TP (1998) A parallel, comparative study of intravenous iron versus intravenous ascorbic acid for erythropoietin-hyporesponsive anemia in haemodialysis patients with iron overload. Nephrol Dial Transplant 13:2867–2872

    Article  CAS  Google Scholar 

  28. 28.

    Teucher B, Olivares M, Cori H (2004) Enhancers of iron absorption: ascorbic acid and other organic acids. Int J Vitam Nutr Res 74(6):403–419

    Article  CAS  Google Scholar 

  29. 29.

    Thannoun AM, Mahoney AW, Hendricks DG, Zhang D (1987) Effect of meat-bread mixture on bioavailability on total dietary iron for anemic rats. Cereal Chem 64:399–403

    CAS  Google Scholar 

  30. 30.

    Theuer RC, Martin WH, Wallande JF, Sarett HP (1973) Effect of processing on availability of iron salts in liquid infant formula products: experimental milk-based formulas. J Agric Food Chem 21:482–485

    Article  CAS  Google Scholar 

  31. 31.

    Verma RS, Motzok I, Chen SS, Rasper J, Ross HU (1977) Effect of storage in flour and of particle size on the bioavailability of elemental iron powders for rats and humans. J Assoc Off Anal Chem 60:759–765

    CAS  Google Scholar 

  32. 32.

    Wegmüller R, Zimmerman MB, Moretti D, Arnold M, Langhans W, Hurrell RF (2004) Particle size reduction and encapsulation affect the bioavailability of ferric pyrophosphate in rats. J Nutr 134:3301–3304

    Google Scholar 

  33. 33.

    Whittaker PG, Hines F, Robi MG, Dunkel VC (1996) Histopathological evaluation of liver, pancreas, spleen, and heart from iron-overloaded Sprague-Dawley rats. Toxicol Pathol 24:558–563

    CAS  Article  Google Scholar 

  34. 34.

    WHO (2002) The world health report 2002—reducing risks, promoting healthy life. WHO, Geneva

    Google Scholar 

  35. 35.

    Zhang D, Hendricks DG, Mahoney AW (1989) Bioavailability of total iron from meat, spinach (Spinacea oleracea L.) and meat-spinach mixture by anemic and non-anemic rats. Br J Nutr 61:331–343

    Article  CAS  Google Scholar 

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Acknowledgments

We are grateful to Hero España S.A. (Murcia, Spain) for providing the samples used in this work and to the Regional Government of Murcia (Consejería de Tecnología, Industria y Comercio) for funding the project INAFIB (Ref. 4I0ISIU09).

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Correspondence to Darío Pérez-Conesa.

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Haro-Vicente, J.F., Pérez-Conesa, D., Rincón, F. et al. Does ascorbic acid supplementation affect iron bioavailability in rats fed micronized dispersible ferric pyrophosphate fortified fruit juice?. Eur J Nutr 47, 470 (2008). https://doi.org/10.1007/s00394-008-0750-7

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Keywords

  • iron bioavailability
  • ascorbic acid
  • ferrous sulfate
  • micronized dispersible ferric pyrophosphate
  • rats