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Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment

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Abstract

Background

Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence.

Aim of the study

This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT).

Methods

Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-d-[3H]-glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot.

Results

One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 µM) or completely (200 µM) restored insulin-stimulated glucose uptake in cells after GO-induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 µM GT increased insulin-stimulated phosphorylation of these key signaling proteins (P < 0.05).

Conclusions

High-dose (200 µM) GT treatment ameliorated oxidative stress-induced insulin resistance in cultured rat L6 skeletal muscle cells.

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Acknowledgments

A.L.C. is supported by a Peter Doherty Post-Doctoral Fellowship from the National Health and Medical Research Council of Australia (NHMRC), M.A.F is a Principal Research Fellow of the NHMRC.

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Authors and Affiliations

  1. Exercise Metabolism Group, School of Medical Sciences, RMIT University, PO Box 71, Bundoora, VIC, 3083, Australia

    Indu Singh, Andrew L. Carey & John A. Hawley PhD

  2. Cellular and Molecular Metabolism Laboratory, Baker Heart Research Institute, Melbourne, VIC, Australia

    Nadine Watson & Mark A. Febbraio

Authors
  1. Indu Singh
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  2. Andrew L. Carey
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  3. Nadine Watson
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  4. Mark A. Febbraio
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  5. John A. Hawley PhD
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Corresponding author

Correspondence to John A. Hawley PhD.

Additional information

I. Singh and A.L. Carey contributed equally.

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Singh, I., Carey, A.L., Watson, N. et al. Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment. Eur J Nutr 47, 387–392 (2008). https://doi.org/10.1007/s00394-008-0739-2

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  • DOI: https://doi.org/10.1007/s00394-008-0739-2

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