The inverse association of peanut consumption and risk markers of CHD (lipids) has been reported however health professionals are still concerned whether hyperlipidemic subjects advised to eat peanuts will have increased serum lipid levels. Tissue factor (TF), the major regulator of normal haemostasis and thrombosis, plays a critical role in haemostasis in all tissues.
Aim of the study
To investigate the effects of peanut consumption on lipid profile, blood Glutathione (GSH), thiobarbituric acid reactive substances (TBARS), haematologic parameters and TF activities in rats fed a high-cholesterol diet.
32 Wistar Albino rats were divided into 4 groups of 8 rats each: 1-Control 2-Control+peanut 3-Hyperlipidemic and 4-Hyperlipidemic+peanut group. At the end of 12 weeks, blood samples were used to evaluate lipid profile, haemostatic parameters, GSH, TBARS and tissue samples were used for the determination of TF activities.
Peanut consumption increased blood GSH both in the control and hyperlipidemic groups; increased HDL-cholesterol and decreased TBARS in the hyperlipidemic group. The addition of peanut to the diet did not change blood lipids, protrombin time, activated partial thromboplastin time or fibrinogen levels significantly both in the control and hyperlipidemic groups. It affected TF activities differently in both groups. It decreased brain and aorta TF activity but increased spleen and kidney TF activity in the control group. It led to significant increases in the TF activity of kidney, spleen and aorta and a significant decrease in the TF activity of brain in the hyperlipidemic group.
Peanut consumption improved GSH and HDL-C levels and decreased TBARS, without increasing other blood lipids in experimental hyperlipidemia. Nevertheless the mechanism of the effect of peanut consumption on the TF activity of tissues remains to be determined.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Thiobarbituric acid reactive substances
Activated partial thromboplastin time
Saturated fatty acids
Albert CM, Willett WC, Manson JE, Hennekens CH (1998) Nut consumption and the risk of sudden and total cardiac death in the physicians health study. Arch Int Med 162(12):1382–1287
Alper CM, Mattes RD (2003) Peanut consumption improves indices of cardiovascular disease risk in healthy adults. J Am Coll Nutr 22(2):133–141
Alturfan AA, Alturfan EE, Dariyerli N, Zengin E, Aytac E, Yigit G, Kokoglu E (2006) Investigation of tissue factor and other hemostatic profiles in experimental hypothyroidism. Endocrine 30(1):63–67
Arafa HMM (2005) Curcumin attenuates diet-induced hypercholesterolemia in rats. Med Sci Moint 11(7):228–234
Bachli E (2000) History of tissue factor. Brit J Haematol 110:248–255
Beeser H (1988) Critical evaluation of the so far experience using the WHO model of prothrombin time callibration and outlook for further development. Hemostasis 18:181–182
Beutler E (1975) Gluthatione: red cell metabolism a manual biochemical methods, 2nd edn. Grune and Stratton, Newyork pp 112–114
Beynen AC, Danse LHJC, Van Leeuwen FXR, Speijera GJA (1986) Cholesterol metabolism and liver pathology in inbred strains of rats fed high cholesterol, high-cholate diet. Nutr Rep Int 34:1079–1087
Bray GA, Popkin BM (1998) Dietary fat intake does affect obesity. Am J Clin Nutr 68:1157–1173
Cooke JP, Singer AH, Tsao P, Zera P, Rowan RA, Billingham ME (1992) Antiatherogenic effects of l-Arginine in the hypercholesterolemic rabbit. J Clin Invest 90:1168–1172
Davidson E, Howard AN, Gresham GA (1961) Blood coagulation studies in rats given diets which produce thrombosis or atherosclerosis. Brit J Exptl Pathol 42:195
Dawson B, Trapp RG (2001) Basic and clinical biostatistics, 3rd edn. McGraw-Hill Book campany, Singapore
Durak I, Koksal I, Kaçmaz M, Buyukkoçak S, Cimen B, Ozturk S (1999) Hazelnut supplementation enhances plasma antioxidant potential and lowers plasma cholesterol levels. Clin Chim Acta 113–115
Fraser GE (1999) Nut consumption, lipids, and risk of a coronary event. Clin Cardiol 22(Suppl.III):III-11–III-15
Fraser GE, Sabate J, Beeson WL, Strahan M (1992) A possible protective effect of nut consumption on risk of coronary heart disease. The adventist health study. Arch Int Med 152:1416–1424
Frings CS, Fendley TW, Dunn RT, Queen CA (1972) Improved determination of total serum lipids by the sulfo-phospho-vanilin reaction. Clin Chem 18(7):673–674
Gjonnaess H, Fagerhol MK (1975) Studies on coagulation and fibrinolysis in pregnancy. Acta Obstet Gynecol Scand 54:363–367
Gordon T, Castelli WT, Hjortland MC, Kannel WB, Dawber TR (1977) High density lipoprotein as a protective factor against coronary heart disease. The framingham study Am J Med 62(5):707–714
Griel AE, Eissenstat B, Juturu V, Hsieh G, Kris-Etherton PM (2004) Improved diet quality with peanut consumption. J Am Coll Nutr 23(6):660–668
Grundy SM, Denke MA (1990) Dietary influences on serum lipids and lipoproteins. J Lipids Res 31:1149–1172
Higgs J (2002) The beneficial role of peanuts in the diet-an update and rethink! Penauts and their role in CHD. Nutr Food Sci J 32(6):214–218
Hu FB, Stampfer MJ, Rimm EB, Colditz GA, Rosner BA, Speizer FE, Hennekens CH, Willett CW (1998) Frequent nut consumption and risk of coronary heart disease in women: prospective cohort study. BMJ 317:1341–1345
Ingram GIC, Hills M (1976) Reference method for the one stage prothrombin time test on human blood. Thromb Haemostas 36:237–238
Kamath S, Lip GYH (2003) Fibrinogen. Biochemistry, epidemiology and determinants. Q J Med 96:711–729
Kocyigit A, Koylu AA, Keles H (2006) Effects of pistachio nuts consumption on plasma lipid profile and oxidative status in healthy volunteers Nutr Metab Cardiovas Dis 16(3):202–209
Mackman N (2004) Role of tissue factor in hemostasis, thrombosis, and vascular development. Arterioscler Thromb Vasc Biol 24:1015–1022
Neofotistos D, Oropezo M, Ts’ao C-H (1998) Stability of plasma for add-on PT and APTT tests. Am J Clin Pathol 109(6):758–763
O’Byrne DJ, Knauft DA, Shireman RB (1997) Low fat-monounsaturated rich diets containing high-oleic peanuts improve serum lipoprotein profiles. Lipids 32(7):687–695
Okada M, Matsui H, Ito Y, Fujiwara A, Inano K (1998) Low-density lipoprotein cholesterol can be chemically measured: a new superior method. J Lab Clin Med 132(3):195–201
Olsen AK, Bladbjerg EM, Hansen AK, Marckmann P (2002) A high fat meal activates blood coagulation factor VII in rats. J Nutr 132:347–350
Prineas RJ, Kushi LH, Falsom AR, Bostick RM, Wu Y, Kushi LH (1993) Walnuts and serum lipids. New Engl J Med 328:603–607
Rauch U, Nemerson Y (2000) Tissue factor, the blood, and the arterial wall. Trends Cardiol Med 10:139–143
Rayssiguier Y, Gueux E (1986) Magnesium and lipids in cardiovascular disease. J Am Coll Nutr 5:507–519
Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolics acids. Free Radic Biol Med 20:933–956
Richmond W (1973) Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 19:1350–1356
Sambola A, Osende J, Hathcock J, Degen M, Nemerson Y, Fuster V, Crandall J, Badimon JJ (2003) Role of risk factors in the modulation of tissue factor activity and blood thrombogenicity. Circulation 107:973–977
Talcott ST, Passeretti S, Duncan CE, Gorbet DW (2005) Polyphenolic content and sensory properties of normal and high oleic acid peanuts. Food Chemistry 90:379–388
Trinder P (1969) Quantitative determination of triglycerides using GPO-PAP method. Ann Biochem 6:24–27
Yagi K (1984) Assay for blood plasma or serum methods in enzymology. Methods Enzymol 105:328–337
Yarat A, Tunalı T, Pisiriciler Akyuz S, Ipbuker A, Emekli N (2004) Salivary thromboplastic activity in diabetics and healthy controls. Clin Oral Invest 8(1):36–39
Zacharski LR, Rosenstein R (1979) Reduction of salivary tissue factor (Thromboplastin) activity by warfarin therapy. Blood 53(3):366–374
This study was financially supported by Grant Project No: SAĞ-080/120503 from the Commission of Scientific Investigations Projects of Marmara University. We thank Prof. Dr. Ahmet Dirican from Istanbul University Cerrahpasa Medical Faculty, Department of Statistics for performing the statistical analysis.
Rights and permissions
About this article
Cite this article
Emekli-Alturfan, E., Kasikci, E. & Yarat, A. Peanuts improve blood glutathione, HDL-cholesterol level and change tissue factor activity in rats fed a high-cholesterol diet. Eur J Nutr 46, 476–482 (2007). https://doi.org/10.1007/s00394-007-0688-1
- tissue factor