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The recovery of 13C-labeled oleic acid in rat lymph after administration of long chain triacylglycerols or specific structured triacylglycerols

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Abstract

Background

Consumption of specific structured triacylglycerols, MLM (M = medium chain fatty acid, L = long chain fatty acid), delivers fast energy and long chain fatty acids to the organism.

Aim of the study

The purpose of the present study was to compare lymphatic absorption of 13C-labeled MLM and 13C-labeled LLL in rats. Stable isotope labeling enables the separation of the endogenous and exogenous fatty acids.

Methods

Lymph was collected during 24 h following administration of MLM or LLL. Lymph fatty acid composition and 13C-enrichment were determined and quantified by gas chromatography combustion isotope ratio mass spectrometry.

Results

The recovery of 18:1n-9 was higher after administration of LLL compared with MLM (58.1% ± 7.4% and 29.1% ± 3.9%, respectively, P < 0.001). This may be due to a higher chylomicron formation stimulated by a higher amount of long chain fatty acids in the intestine after LLL compared with MLM administration. This was confirmed by the tendencies of higher lymphatic transport of endogenous fatty acids.

Conclusion

The study revealed a higher lymphatic recovery of the administered long chain fatty acids after LLL compared with MLM consumption.

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Acknowledgments

We thank Xuebing Xu for preparing the unlabeled structured triacylglycerols and Zahra Roudaki, Karen Jensen, Lillian Vile, and Egon Christensen for technical assistance.

The present address of Bodil Vistisen is: The Division of Pathophysiology, The Department of Medical Physiology, the PANUM Institute, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N.

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Authors and Affiliations

  1. BioCentrum-DTU, Section of Biochemistry & Nutrition, Technical University of Denmark, Lyngby, Denmark

    Bodil Vistisen, Huiling Mu & Carl-Erik Høy

  2. Division of Patophysiology, Dept. of Medical PhysiologyPANUM Institute, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen N, Denmark

    Bodil Vistisen

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  1. Bodil Vistisen
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Correspondence to Bodil Vistisen.

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Vistisen, B., Mu, H. & Høy, CE. The recovery of 13C-labeled oleic acid in rat lymph after administration of long chain triacylglycerols or specific structured triacylglycerols. Eur J Nutr 45, 363–368 (2006). https://doi.org/10.1007/s00394-006-0607-x

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  • DOI: https://doi.org/10.1007/s00394-006-0607-x

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