Abstract
Purpose
The aim of this study was to evaluate the prevalence and clinical significance of caspase-10 mRNA expression in stage II colorectal cancer.
Methods
Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze caspase-10 expression in cancer tissue and corresponding normal mucosa from 120 patients with stage II colorectal cancer. Variables were analyzed by Chi-square test or Fisher’s exact test. Survival was evaluated with method of Kaplan–Meier. Multivariate analysis was performed with Cox’s proportional hazards model.
Results
The expression of caspase-10 mRNA was found to be downregulated in cancer tissue compared to normal mucosa (P = 0.001). Poorly differentiated cancer showed lower mRNA expression than cancer with greater differentiation (P = 0.031). Univariate survival curves, estimated using the method of Kaplan–Meier, defined a significant association between caspase-10 expression and both overall survival (P = 0.012) and disease-free survival (P = 0.021). A multivariate analysis, performed by Cox’s proportional hazards regression model, confirmed that a low caspase-10 expression was the only significant factor to predict poor prognosis in patients with stage II colorectal cancer.
Conclusion
Our data indicate that caspase-10 expression, measured by quantitative real-time RT-PCR, is a possible prognostic factor in patients with stage II colorectal cancer.
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Acknowledgments
We thank professors Zhou and Wang for guiding this study, the National Key Laboratory of Biotherapy for useful assistance, and the Department of Gastrointestinal Surgery for providing the experimental specimens. This work was supported by the National Natural Science Founding of China, No. 30801331.
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The authors declare that they have no conflict of interest.
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Shen, XG., Wang, C., Li, Y. et al. Downregulation of caspase-10 predicting poor survival after resection of stage II colorectal cancer. Int J Colorectal Dis 26, 1519–1524 (2011). https://doi.org/10.1007/s00384-011-1239-z
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DOI: https://doi.org/10.1007/s00384-011-1239-z