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Wnt5a expression in the hindgut of fetal rats with chemically induced anorectal malformations—studies in the ETU rat model

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

Wnt5a is involved in the initiating and patterning morphological adaptations of gut. However, its role remained unknown during terminal hindgut development in the normal and anorectal malformation (ARM) rat embryos. This study was designed to investigate the expression pattern of Wnt5a in the terminal hindgut in ARM rat embryos.

Materials and methods

Ethylenethiourea-induced ARM model was introduced to investigate the expression pattern of Wnt5a during terminal hindgut development using immunohistochemical staining, reverse transcriptase polymerase chain reaction (RT-PCR), and Western blot analysis.

Results

Immunostaining revealed that Wnt5a expression showed space-dependent changes in the developing terminal hindgut. On embryonic day 17 (E17) in normal embryos, the Wnt5a protein was initially expressed in the mesenchyme of the terminal hindgut. From E18 to 19, the positive staining cells gradually increased. The expression was detected mainly in the circular muscle and myenteric plexus of hindgut. In the ARM embryos, on E17, the Wnt5a protein was also expressed in the hindgut. However, from E18 to 19, the positive staining cells in the middle hindgut gradually increased but in the terminal hindgut decreased. In Western blot and RT-PCR, time-dependent changes of Wnt5a protein and mRNA expression were remarkable during the terminal hindgut development in normal and ARM embryos.

Conclusion

These data implied that the downregulation of Wnt5a at the time of hindgut neuromuscular development might partly be related to the maldevelopment of terminal hindgut in ARM.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (grant nos. 30801199 and 30872704), Province Science and Technology in the Liaoning Offends Pass Item (grant no. 2007225005-3), Project of Key Laboratory of the Education Department of Liaoning Province (grant no. LS2010171), and Outstanding Scientific Research of Shengjing Hospital of China Medical University (200811).

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Authors and Affiliations

  1. Department of Pediatric Surgery, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004, People’s Republic of China

    Huimin Jia, Qingjiang Chen, Tao Zhang, Yuzuo Bai & Weilin Wang

  2. The Key Laboratory of Health Ministry for Congenital Malformation, No. 36 Sanhao Street, Heping District, Shenyang, 110004, People’s Republic of China

    Zhengwei Yuan

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  1. Huimin Jia
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  2. Qingjiang Chen
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  3. Tao Zhang
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  4. Yuzuo Bai
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  5. Zhengwei Yuan
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  6. Weilin Wang
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Correspondence to Weilin Wang.

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Jia, H., Chen, Q., Zhang, T. et al. Wnt5a expression in the hindgut of fetal rats with chemically induced anorectal malformations—studies in the ETU rat model. Int J Colorectal Dis 26, 493–499 (2011). https://doi.org/10.1007/s00384-010-1125-0

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  • DOI: https://doi.org/10.1007/s00384-010-1125-0

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