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The protective effect of oral colitis-derived proteins in a murine model of inflammatory bowel disease is associated with an increase in γδ T cells in large intestinal mucosa

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Backgrounds and aims

Oral tolerance has previously been shown effective in preventing several immune-mediated disorders in animal models. The aims of this study were to investigate the effect of oral colitis-extracted proteins (CEP) on dextran sulfate sodium (DSS)-induced colitis in BALB/c mice and to explore the relative role of the intestinal mucosal γδ T cells.

Methods

The effect of five low oral doses of CEP on colitis was evaluated by clinical manifestation and histological lesions. Serum cytokines were measured by enzyme-linked immunosorbent assay. The percentages of the intestinal mucosal γδ T cells were evaluated by flow cytometry.

Results

CEP-fed colitis mice showed less severe symptoms and histological injury than bovine serum albumin (BSA)-fed control mice. Tolerized mice developed an increase in TGF-β1 and no change in IFN-γ serum levels. Increases in TCRγδ+ T cells and CD8α+TCRγδ+ T cells in small intestinal mucosal lymphocytes and no quantitative change in large intestinal mucosal lymphocytes were demonstrated in colitis mice compared to untreated mice. The proportions of TCRγδ+ T cells and CD8α+TCRγδ+ T cells in large intestinal mucosal lymphocytes from CEP-fed colitis mice were significantly higher compared to BSA-fed controls. The disease activity index negatively correlated with the percentages of large intestinal mucosal γδ T cells. Furthermore, mucosal repair in repair-period mice was also accompanied by increases in TCRγδ+ T cells and CD8α+TCRγδ+ T cells in large intestinal mucosal lymphocytes.

Conclusion

Improvement of DSS-induced colitis that resulted from oral administration of colitis-extracted proteins is associated with an increase in γδ T cells in large intestinal mucosa.

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Acknowledgments

We thank Dr. Hequan Li at the Department of Respirology for helping in the critical reading and writing techniques of the manuscript.

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Authors and Affiliations

  1. Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, People’s Republic of China

    Yuefang Ye, Xi Jin, Min Yue, Shaohua Chen, Chaohui Yu & Youming Li

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  1. Yuefang Ye
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  2. Xi Jin
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  3. Min Yue
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  5. Chaohui Yu
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  6. Youming Li
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Correspondence to Youming Li.

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Ye, Y., Jin, X., Yue, M. et al. The protective effect of oral colitis-derived proteins in a murine model of inflammatory bowel disease is associated with an increase in γδ T cells in large intestinal mucosa. Int J Colorectal Dis 25, 1055–1062 (2010). https://doi.org/10.1007/s00384-010-0975-9

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