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The frequency of C3435T MDR1 gene polymorphism in Iranian patients with ulcerative colitis

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Abstract

Back ground and Aims

The MDR1 (multidrug resistance) gene, located on chromosome 7, is in one of the inflammatory bowel disease susceptibility loci. It produces P-glycoprotein, a transmembrane efflux pump, transferring drugs and toxins from intracellular to extracellular domains. In the human gastrointestinal (GI) tract, P-glycoprotein is found in high concentrations on the epithelial cells of the colon and small intestine. MDR1 gene polymorphisms such as C3435T are associated with lower P-glycoprotein expression, and thus it is suggested to have an association with ulcerative colitis. We tried to determine the frequency of C3435T polymorphism of the MDR1 gene in Iranian patients with ulcerative colitis and to compare it with a healthy control population.

Materials and methods

In this case–control-designed study, 300 unrelated ulcerative colitis patients and 300 sex-and-age-matched healthy controls were enrolled. They were visited at a tertiary center during a 2-year period (2003–2005). DNA of patients and controls was amplified by polymerase chain reaction with specific primers, and C3435T polymorphism was detected by the restriction fragment length polymorphism method.

Results

The frequency of the 3435T allele was significantly higher in ulcerative colitis patients compared to the controls (p < 0.001). The frequency of homozygote T/T and heterozygote C/T genotypes were also significantly higher in Iranian patients with ulcerative colitis (p = 0.044 and 0.041, respectively).

Conclusion

This study suggests that C3435T polymorphism of the MDR1 gene has an association with ulcerative colitis in Iranian population as previously reported in western countries.

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Authors and Affiliations

  1. Research Centre for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

    Alma Farnood, Nosratollah Naderi, Seyed Javad Mirhasani Moghaddam, Babak Noorinayer, Farzad Firouzi, Rahim Aghazadeh & Mohammad Reza Zali

  2. Gastroenterology department, Tehran University of Medical Sciences, Tehran, Iran

    Nasser Ebrahimi daryani

Authors
  1. Alma Farnood
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  2. Nosratollah Naderi
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  3. Seyed Javad Mirhasani Moghaddam
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  4. Babak Noorinayer
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  5. Farzad Firouzi
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  7. Nasser Ebrahimi daryani
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  8. Mohammad Reza Zali
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Correspondence to Alma Farnood.

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Farnood, A., Naderi, N., Moghaddam, S.J.M. et al. The frequency of C3435T MDR1 gene polymorphism in Iranian patients with ulcerative colitis. Int J Colorectal Dis 22, 999–1003 (2007). https://doi.org/10.1007/s00384-007-0270-6

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  • DOI: https://doi.org/10.1007/s00384-007-0270-6

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